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A novel method to modulate desensitization and truncation of luteinizing hormone receptors using antisense oligodeoxynucleotides.

Abstract：

:We report a novel method to study the mechanisms of luteinizing hormone (LH) receptor desensitization and truncation, using antisense oligodeoxynucleotides that code for regions of the NH2-terminus, the third extracellular loop and the C-terminus of the LH receptor. Mouse tumour (MA10) Leydig cells were incubated for 48 h with the addition of 2.5 microM antisense oligodeoxynucleotides at time 0 and 24 h. It was found that the NH2-terminus oligodeoxynucleotide completely inhibited synthesis of the LH receptor. Pretreatment with the third extracellular loop oligodeoxynucleotide inhibited LH-, dibutyrylcyclic AMP (db-cAMP)- and phorbol 12-myristate 13-acetate (PMA)-induced desensitization and truncation of LH receptors. Truncation but not desensitization, of the LH receptor was prevented in cells pretreated with the C-terminus oligodeoxynucleotide. These results indicate that different sites of the C-terminal intracellular tail of the LH receptor are involved in the regulation of desensitization and truncation of the LH receptor.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

West AP,Cooke BA

doi

10.1016/0303-7207(91)90111-5

keywords:

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

R9-14

issue

1-3

eissn

0303-7207

issn

1872-8057

pii

0303-7207(91)90111-5

journal_volume

79

pub_type

杂志文章

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