Androgen metabolism in relation to growth stimulation by a uterine cell line.

abstract：:We have designed and synthesized a biotinylated vasopressin antagonist which is a selective probe for studying the V1a subtype of vasopressin receptor. Initially we synthesized the novel vasopressin analogue d(CH2)5Tyr(Me)2LysNH2(9)AVP (ALVP). Biotinamidocaproate was subsequently coupled to the epsilon-amino group of ...

journal_title：Molecular and cellular endocrinology

authors： Howl J,Kerr ID,Chan CH,Wheatley M

更新日期：1991-05-01 00:00:00

abstract：:Although estradiol bone contribution has been deeply studied, little is known about the action of estrone. We investigated the direct action of estrone on osteoblasts growth and differentiation, with focus on the biochemical mechanism displayed by the estrogen. Murine calvarial osteoblast cultures in vitro exposed to ...

journal_title：Molecular and cellular endocrinology

authors： Crescitelli MC,Rauschemberger MB,Cepeda S,Sandoval M,Massheimer VL

更新日期：2019-12-01 00:00:00

abstract：:The effects of propylthiouracil (PTU) treatment on vasopressin, angiotensin II, glucagon and alpha 1-adrenergic receptors in both developing and adult rats were studied in liver membrane preparations by measuring the binding of the following ligands: [3H][8-lysine]vasopressin, [3H]Sar-angiotensin II, [125I]glucagon an...

journal_title：Molecular and cellular endocrinology

authors： Ali M,Cantau B,Chicot D,Clos J

更新日期：1987-05-01 00:00:00

abstract：:Bone tissue is especially receptive to physical stimulation and agents with the capacity to mimic the signalling incurred via mechanical loading on osteoblasts may find an application in a bone regenerative setting. Recently this laboratory revealed that the major serum lipid, lysophosphatidic acid (LPA), co-operated ...

journal_title：Molecular and cellular endocrinology

authors： Mansell JP,Farrar D,Jones S,Nowghani M

更新日期：2009-06-16 00:00:00

abstract：:Menin is a protein product of a tumor supressor gene MEN1, mutations of which are responsible for multiple endocrine neoplasia type 1, an autosomal dominant familial cancer syndrome. We determined the nucleotide sequence of the Drosophila menin cDNA using RT-PCR and RACE, and confirmed it by direct sequencing of genom...

journal_title：Molecular and cellular endocrinology

authors： Maruyama K,Tsukada T,Honda M,Nara-Ashizawa N,Noguchi K,Cheng J,Ohkura N,Sasaki K,Yamaguchi K

更新日期：2000-10-25 00:00:00

abstract：:Most secreted proteins are modified post-translationally with the addition of carbohydrate. It has been difficult to use crystallography to solve the structures of these proteins due to the inherent heterogeneity of the carbohydrate. The structure of the chemically deglycosylated form (hydrogen fluoride treated) of hu...

journal_title：Molecular and cellular endocrinology

authors： Lustbader JW,Pollak S,Lobel L,Trakht I,Homans S,Brown JM,Canfield RE

更新日期：1996-12-20 00:00:00

abstract：:Vitamin D is a key factor for calcium and bone homeostasis, but signalling through the vitamin D receptor (VDR) seems also to be important for testicular function. To test the functional role of vitamin D signalling we examined the male reproductive system of the Leuven Vdr-ablated (Vdr(-/-)) mice, previously establis...

journal_title：Molecular and cellular endocrinology

authors： Blomberg Jensen M,Lieben L,Nielsen JE,Willems A,Jørgensen A,Juul A,Toppari J,Carmeliet G,Rajpert-De Meyts E

更新日期：2013-09-05 00:00:00

abstract：:Sex steroids have been known to play important roles in the homeostasis of human skin, but little is known about their biosyntheses in that tissue. In this study, we characterized the correlation between the concentrations of sex steroids and the expression levels of the factors involved in their synthesis or metaboli...

journal_title：Molecular and cellular endocrinology

authors： Inoue T,Miki Y,Abe K,Hatori M,Hosaka M,Kariya Y,Kakuo S,Fujimura T,Hachiya A,Honma S,Aiba S,Sasano H

更新日期：2012-10-15 00:00:00

abstract：:The relationship between the structure of human growth hormone (hGH) and the hormone-receptor interaction has been investigated using as probes monoclonal antibodies (Mabs) to hGH of defined epitope specificity profile. Seven high affinity Mabs were studied for their ability (i) to inhibit the binding of 125I-hGH to N...

journal_title：Molecular and cellular endocrinology

authors： Strasburger CJ,Binder L,Elberg G,Cohen-Chapnik N,Kohen F,Gertler A

更新日期：1989-11-01 00:00:00

abstract：:The effects of cholera toxin on the responses of cultured Sertoli cells were compared with those elicited by follicle-stimulating hormone (FSH), and N6O2'-dibutyryl-3',5'-cyclic AMP (bu2cAMP). Addition of FSH or cholera toxin increased cAMP levels. Subsequently there was greater rates of conversion of testosterone to ...

journal_title：Molecular and cellular endocrinology

authors： Fritz IB,Griswold MD,Louis BG,Dorrington JH

更新日期：1976-08-01 00:00:00

abstract：:Visceral obesity is an independent risk factor for metabolic syndrome, and abnormal fat accumulation is linked to increases in the number and size of adipocytes. MiR-146b was a miRNA highly expressed in mature adipocytes while very lowly expressed in human mesenchymal stem cells (hMSCs) and human visceral preadipocyte...

journal_title：Molecular and cellular endocrinology

authors： Chen L,Dai YM,Ji CB,Yang L,Shi CM,Xu GF,Pang LX,Huang FY,Zhang CM,Guo XR

更新日期：2014-08-05 00:00:00

abstract：:We reported previously that stem Leydig cells (SLC) on the surfaces of rat testicular seminiferous tubules are able to differentiate into Leydig cells. The proliferation and differentiation of SLCs seem likely to be regulated by niche cells, including nearby germ and Sertoli cells. Due to the cyclical nature of sperma...

journal_title：Molecular and cellular endocrinology

authors： Guan X,Chen F,Chen P,Zhao X,Mei H,Liu J,Lian Q,Zirkin BR,Chen H

更新日期：2019-02-05 00:00:00

abstract：:The extracellular matrix (ECM) component hyaluronan (HA) is considered to contribute to the optimal development of the gametes and the embryo. In this study, the regulation and localization of HA, its synthases (HAS 1-3) and the receptors CD44, RHAMM, and putative HARE were investigated in bovine oviducts during the e...

journal_title：Molecular and cellular endocrinology

authors： Ulbrich SE,Schoenfelder M,Thoene S,Einspanier R

更新日期：2004-02-12 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPAR)α have been shown to exert immunomodulatory effects in autoimmune disorders; no study evaluated the effect of PPARα activation in Graves' ophthalmopathy (GO). We show the presence of PPARα, δ and γ in GO fibroblasts and preadipocytes. PPARα activators have a potent inh...

journal_title：Molecular and cellular endocrinology

authors： Antonelli A,Ferrari SM,Frascerra S,Ruffilli I,Gelmini S,Minuto M,Pupilli C,Miccoli P,Sellari-Franceschini S,Ferrannini E,Fallahi P

更新日期：2012-02-26 00:00:00

abstract：:Hypothalamic dysfunction is a common feature of experimental obesity. Studies have identified at least three mechanisms involved in the development of hypothalamic neuronal defects in diet-induced obesity: i, inflammation; ii, endoplasmic reticulum stress; and iii, mitochondrial abnormalities. However, which of these ...

journal_title：Molecular and cellular endocrinology

authors： Carraro RS,Souza GF,Solon C,Razolli DS,Chausse B,Barbizan R,Victorio SC,Velloso LA

更新日期：2018-01-15 00:00:00

abstract：:Lymphocyte cell lines were established from five patients with vitamin D-dependent rickets, type II (VDDR-II). These lines were established by infection with human T-lymphotrophic virus type I (HTLV-I). Binding of [3H]1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) to its receptor in these cell lines was compared to bind...

journal_title：Molecular and cellular endocrinology

authors： Koeffler HP,Bishop JE,Reichel H,Singer F,Nagler A,Tobler A,Walka M,Norman AW

更新日期：1990-03-26 00:00:00

abstract：:The functional significance of two unlinked human vitamin D receptor (hVDR) gene polymorphisms was evaluated in twenty human fibroblast cell lines. Genotypes at both a Fok I restriction site (F/f) in exon II and a singlet (A) repeat in exon IX (L/S) were determined, and relative transcription activities of endogenous ...

journal_title：Molecular and cellular endocrinology

authors： Whitfield GK,Remus LS,Jurutka PW,Zitzer H,Oza AK,Dang HT,Haussler CA,Galligan MA,Thatcher ML,Encinas Dominguez C,Haussler MR

更新日期：2001-05-25 00:00:00

abstract：:The adenosine 3', 5'-cyclic monophosphate (cyclic AMP)-dependent protein phosphokinase of rat interstitial cells was characterized by ion-exchange chromatography and sucrose density gradient centrifugation. The 0.2 M NaCl fraction from DEAE-Sephadex showed a small 2.9-S peak of basal enzyme activity, and a large 6.5-S...

journal_title：Molecular and cellular endocrinology

authors： Podesta EJ,Dufau ML,Catt KJ

更新日期：1976-06-01 00:00:00

abstract：:The growth of ZR-75-1 cells, a line of human breast cancer cells in culture, is stimulated by oestradiol and inhibited by anti-oestrogens. Changes in growth rate caused by these agents are accompanied by changes in activity of ornithine decarboxylase, a rate-limiting enzyme for polyamine synthesis. Furthermore, the gr...

journal_title：Molecular and cellular endocrinology

authors： Hoggard N,Green CD

更新日期：1986-06-01 00:00:00

abstract：:At the early stage of its development in 1957, the daily dose of spironolactone necessary to improve various pathological conditions was not precisely determined and dose-dependent sexual side effects limited its long-term use. Prescription of high daily doses and absence of selectivity for the mineralocorticoid recep...

journal_title：Molecular and cellular endocrinology

authors： Ménard J

更新日期：2004-03-31 00:00:00

abstract：:Adrenocortical cancer is a rare malignancy for which current pharmacological therapies are still insufficient. We tested the effect of a novel PI3 kinase - mammalian target of rapamycin dual inhibitor (NVP-BEZ235) on proliferation of the H295R adrenocortical cancer cell line in vitro and grown as xenografts in immunod...

journal_title：Molecular and cellular endocrinology

authors： Doghman M,Lalli E

更新日期：2012-11-25 00:00:00

abstract：:Pituitary cultures from adult rats contain two subtypes of prolactin (PRL) cells, small-plaque (SP) and large-plaque (LP) lactotropes, which exhibit distinct rates of basal secretion and thereby form PRL plaques of different sizes in reverse hemolytic plaque assay experiments. In the present study, we have used plaque...

journal_title：Molecular and cellular endocrinology

authors： Horta J,Cota G

更新日期：1993-04-01 00:00:00

abstract：:Receptors for calcitriol are described in human decidua. They exhibit a dissociation constant of 35 +/- 6 pM and they are at concentrations similar to those found in other tissues (0.45 +/- 0.04 fmol/micrograms DNA). They are highly specific for calcitriol since neither of the other vitamin D3 derivatives nor the ster...

journal_title：Molecular and cellular endocrinology

authors： Delvin EE,Gagnon L,Arabian A,Gibb W

更新日期：1990-07-09 00:00:00

abstract：:This study investigated the effects of SOD2 (MnSOD)-deficiency-induced excessive oxidative stress on ovarian steroidogenesis in vivo and isolated and cultured granulosa cells using WT and Sod2+/- mice. Basal and 48 h eCG-stimulated plasma progesterone levels were decreased ~50% in female Sod2+/- mice, whereas plasma p...

journal_title：Molecular and cellular endocrinology

authors： Zaidi SK,Shen WJ,Cortez Y,Bittner S,Bittner A,Arshad S,Huang TT,Kraemer FB,Azhar S

更新日期：2021-01-01 00:00:00

abstract：:Over the last 20 years, aromatase inhibitors have been developed to become a highly effective treatment strategy for treatment of hormone receptor positive breast cancer. Despite their success, poor response and resistance limit the effectiveness of these agents in up to 50% of patients. In recent years, studies using...

journal_title：Molecular and cellular endocrinology

authors： Dunbier AK,Martin LA,Dowsett M

更新日期：2011-07-04 00:00:00

abstract：:A new strain, named WRT cells, has been generated from primary cultures of rat thyroids. The primary culture was grown in Coon's modified Ham's F12 medium with 5% calf serum, insulin, hydrocortisone, transferrin, somatostatin, glycyl-L-histidyl-L-lysine and thyrotropin (TSH). On the basis of the following facts, the W...

journal_title：Molecular and cellular endocrinology

authors： Brandi ML,Rotella CM,Mavilia C,Franceschelli F,Tanini A,Toccafondi R

更新日期：1987-11-01 00:00:00

abstract：:The oligosaccharide chains of pituitary glycoprotein hormones such as human thyroid-stimulating hormone (hTSH) have been shown to be important in biosynthesis, subunit association, secretion and bioactivity. However, the exact biological significance of these glycosylation variants (isoforms) remains controversial. Th...

journal_title：Molecular and cellular endocrinology

authors： Schaaf L,Leiprecht A,Saji M,Hübner U,Usadel KH,Kohn LD

更新日期：1997-09-19 00:00:00

abstract：:Transthyretin (TTR) transports thyroid hormones (THs), thyroxine (T4) and triiodothyronine (T3) in the blood of vertebrates. TH-binding sites are highly conserved in vertebrate TTR, however, piscine TTR has a longer N-terminus which is thought to influence TH-binding affinity and may influence TTR stability. We produc...

journal_title：Molecular and cellular endocrinology

authors： Morgado I,Melo EP,Lundberg E,Estrela NL,Sauer-Eriksson AE,Power DM

更新日期：2008-11-25 00:00:00

abstract：:Spermatogonial transplantation as developed in the laboratory of Ralph Brinster has been a technological breakthrough in the study of Sertoli-germ cell interactions. For the first time, germ cells can be transferred from one animal to another and from one species to another. The transfer technology combined with devel...

journal_title：Molecular and cellular endocrinology

authors： Russell LD,Griswold MD

更新日期：2000-03-30 00:00:00

abstract：:Tissue slices or dispersed cells of bovine parathyroid gland were incubated with [3H]leucine to label the intracellular proteins and then tested for their secretory response to isoproterenol and cycloheximide at different calcium concentrations. Secretion of the newly synthesized as well as the older PTH and SP-I was ...

journal_title：Molecular and cellular endocrinology

authors： Chu LL,MacGregor RR,Hamilton JW

更新日期：1983-12-01 00:00:00