Abstract:
:The adenosine 3', 5'-cyclic monophosphate (cyclic AMP)-dependent protein phosphokinase of rat interstitial cells was characterized by ion-exchange chromatography and sucrose density gradient centrifugation. The 0.2 M NaCl fraction from DEAE-Sephadex showed a small 2.9-S peak of basal enzyme activity, and a large 6.5-S peak of cyclic AMP-dependent protein kinase activity; fractions eluted from DEAE-Sephadex with 0.3-0.5 M NaCl contained a major 3.8-S peak of cyclic AMP-dependent enzyme activity. Activation of protein kinase in cell extracts by cyclic AMP, and in intact interstitial cells by trophic hormone, caused a major shift of enzyme activity to the 2.9-S cyclic AMP-dependent form which was eluted from DEAE-Sephadex by 0.2 M NaCl. These results are consistent with the presence of two distinct protein kinase holoenzymes, with a common 2.9-S catalytic subunit. During hormonal activation of protein kinase in dispersed interstitial cells by 10-10 M human chorionic gonadotropin (hCG), conversion to the 2.9-S catalytic subunit was observed between 2 and 30 min of incubation. Protein kinase activity was correlated with cyclic AMP production, and full enzyme activation occurred at the time of maximum intracellular cyclic AMP concentration. The presence of two forms of cyclic AMP-dependent protein kinase in the Leydig cell provides a potential mechanism whereby progressive occupancy of gonadotropin receptors could evoke a series of discrete target cell responses.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Podesta EJ,Dufau ML,Catt KJdoi
10.1016/0303-7207(76)90074-5subject
Has Abstractpub_date
1976-06-01 00:00:00pages
109-22issue
1-2eissn
0303-7207issn
1872-8057journal_volume
5pub_type
杂志文章abstract::Several common KCNQ1 gene polymorphisms have been associated with the risk of type 2 diabetes (T2DM) and diabetic nephropathy. This effect is explained by the role of the kcnq1 protein as a potassium channel that in the pancreatic beta-cells drives an electrical signal that facilitates glucose-stimulated insulin secre...
journal_title:Molecular and cellular endocrinology
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abstract::Aldosterone and cortisol are the major mineralocorticoid and glucocorticoid produced by the human adrenal. Circulating levels of angiotensin II and potassium control the adrenal production of aldosterone, while the production of cortisol is controlled mainly by adrenocorticotropin. The capacity of the adrenal cortex t...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章,评审
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2012-11-25 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/j.mce.2016.06.007
更新日期:2016-09-15 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2018-09-15 00:00:00
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更新日期:2009-03-05 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(87)90125-0
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
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更新日期:2017-11-05 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
abstract::Interaction of tamoxifen, trioxifene and LY117018 with cytosol-estrogen receptors from immature rat uteri was compared. Determination of relative binding affinity (RBA) by competition with [3H] estradiol under various assay conditions revealed that the RBA of LY117018 increased with temperature while that of trioxifen...
journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(81)90105-2
更新日期:1981-04-01 00:00:00
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更新日期:1998-06-25 00:00:00
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journal_title:Molecular and cellular endocrinology
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更新日期:2007-07-15 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2009-12-10 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2002-11-29 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1975-08-01 00:00:00