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Human corticotropin-releasing factor type 1 receptor analysis with white blood cells mRNAs and DNA.

Abstract：

:The human corticotropin-releasing factor receptor type 1 (hCRF-R1) functional transcript is mainly expressed in the anterior pituitary corticotrophs, a tissue usually not available for clinical investigation. Splice variants translated into defective isoforms of the receptor have been described in few peripheral tissues. The aim of this work was to determine whether peripheral white blood cells from healthy individuals, an accessible tissue for clinical investigation, were suitable for the analysis of the hCRF-R1 transcript and gene. We report that: (i) specific amplification of the hCRF-R1 transcript from peripheral white blood cells mRNAs is feasible; (ii) this transcript is similar to the functional transcript; (iii) the draft sequence of chromosome 17 and unrelated sequences allow direct sequencing of all 14 exons of the gene, adjacent splice sites and related branch points. In conclusion, these approaches would be suitable for studies in patients having isolated secondary glucocorticoids deficiency to implicate the hCRH-R1 in the etiology of the disease.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Ben Simon E,Besancon R,Benetollo C,Nicolino M,Saez JM,Li JY

doi

10.1016/s0303-7207(02)00359-3

keywords:

subject

Has Abstract

pub_date

2003-01-31 00:00:00

pages

189-93

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303720702003593

journal_volume

199

pub_type

杂志文章

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