Isolation of cells from rat seminal vesicles and epididymis and their use in studying androgen action.

abstract：:The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR α and PPAR β/δ activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR ...

journal_title：Molecular and cellular endocrinology

authors： Regueira M,Riera MF,Galardo MN,Pellizzari EH,Cigorraga SB,Meroni SB

更新日期：2014-01-25 00:00:00

abstract：:The red-spotted grouper, Epinephelus akaara, is a protogynous hermaphroditic fish that shows the characteristic of natural sex change. In this study, 2-year-old female groupers were successfully reversed to functional males by oral administration of 17alpha-methyltestosterone (MT) for 42 days. The protein inhibitor of...

journal_title：Molecular and cellular endocrinology

authors： Li SW,Long ZF,Du J,Liu SG,Wen JJ

更新日期：2009-10-15 00:00:00

abstract：:Signaling pathways initiated by transforming growth factor-β (TGF-β) and insulin-like growth factors (IGFs) are important in osteosarcoma cell growth. We have investigated a role for endogenous IGF binding protein-3 (IGFBP-3) in mediating cross-talk between TGF-β receptor and type I IGF receptor (IGF1R) signaling path...

journal_title：Molecular and cellular endocrinology

authors： Schedlich LJ,Yenson VM,Baxter RC

更新日期：2013-09-05 00:00:00

abstract：:Renin secretion was stimulated in rats by adrenalectomy (ADX), by treatment with the converting enzyme inhibitor enalapril (MK 421), or by feeding a low salt diet plus furosemide treatment (FUR). Relative amounts of renin mRNA were measured by densitometric Northern blot analysis using a 32P-labeled 700 bp mouse subma...

journal_title：Molecular and cellular endocrinology

authors： Ludwig G,Ganten D,Murakami K,Fasching U,Hackenthal E

更新日期：1987-04-01 00:00:00

abstract：:17Beta hydroxysteroid dehydrogenase type 7 (HSD17B7) was described to possess dual functionality in steroidogenesis as well as in postsqualene cholesterol biosynthesis in vitro. In order to gain insight into the transcriptional regulation, and thereby into in vivo functionality of HSD17B7, we analyzed and compared the...

journal_title：Molecular and cellular endocrinology

authors： Ohnesorg T,Adamski J

更新日期：2006-03-27 00:00:00

abstract：:Central leptin action requires PI3K activity to modulate glucose homeostasis and peripheral metabolism. However, the mechanism behind this phenomenon is not clearly understood. We hypothesize that hypothalamic PI3K activity is important for the modulation of the AMP-activated protein kinase (AMPK)/acetyl-CoA carboxyla...

journal_title：Molecular and cellular endocrinology

authors： Roman EA,Reis D,Romanatto T,Maimoni D,Ferreira EA,Santos GA,Torsoni AS,Velloso LA,Torsoni MA

更新日期：2010-01-15 00:00:00

abstract：:Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose tr...

journal_title：Molecular and cellular endocrinology

authors： Kemp HF,Hundal HS,Taylor PM

更新日期：1997-04-04 00:00:00

abstract：:Two cAMP-dependent protein kinase activities can be separated from rat thyroid cytosol. Their elution properties during the gel chromatography, as well as their sedimentation coefficients after sucrose-gradient ultracentrifugation and their capacity to dissociate in the presence of histones, suggest they are like the ...

journal_title：Molecular and cellular endocrinology

authors： Delbauffe D,Ohayon R,Pavlovic-Hournac M

更新日期：1979-05-01 00:00:00

abstract：:The rate of dissociation of recombinant, purified human estrogen receptor alpha (ERalpha) from a fluorescein-labeled DNA containing the consensus vitellogenin ERE sequence (F-vitERE) was determined in real time using fluorescence anisotropy. The complex of estradiol-occupied ERalpha with F-vitERE had an apparent disso...

journal_title：Molecular and cellular endocrinology

authors： Szatkowski Ozers M,Hill JJ,Ervin K,Royer CA,Gorski J

更新日期：2001-04-25 00:00:00

abstract：:Recent evidences point to a role of estrogens in males. We have earlier reported that tamoxifen, a synthetic non-steroidal antiestrogen, when administered to adult male rats, in the dose range of 0.04-0.4 mg/kg per day, reduced fertility. The reduced fertility was measured in terms of fertility index (a measure of the...

journal_title：Molecular and cellular endocrinology

authors： Balasinor N,Parte P,Gill-Sharma MK,Juneja HS

更新日期：2001-06-10 00:00:00

abstract：:Transgenic mice carrying a fused gene of the 294-base upstream and 68-base leader sequences of a rat calmodulin gene, CaMII, and beta-galactosidase gene were made. Only spermatocytes expressed the transgene mRNA in the testes of four independent transgenic lines. The localization of transgene mRNA was consistent with ...

journal_title：Molecular and cellular endocrinology

authors： Ikeshima H,Shimoda K,Matsuo K,Hata J,Maejima K,Takano T

更新日期：1994-02-01 00:00:00

abstract：:South African Angora goats are susceptible to cold stress, due to their inability to produce sufficient levels of cortisol. During adrenal steroidogenesis the production of cortisol relies on the activity of two key enzymes, namely cytochrome P450 17alpha-hydroxylase and 3beta-hydroxysteroid dehydrogenase. Cytochrome ...

journal_title：Molecular and cellular endocrinology

authors： Goosen P,Swart AC,Storbeck KH,Swart P

更新日期：2010-02-05 00:00:00

abstract：:Type 4 phosphodiesterases (PDE4s) of the large PDE enzyme superfamily have unique specificity for cAMP and may, therefore, be relevant for somatotroph tumorigenesis. Somatotroph adenomas typically overexpress PDEs probably as part of a compensatory mechanism to reduce cAMP levels. The rat PDE4A5 isoform (human homolog...

journal_title：Molecular and cellular endocrinology

authors： Bizzi MF,Pinheiro SVB,Bolger GB,Schweizer JROL,Giannetti AV,Dang MN,Ribeiro-Oliveira A Jr,Korbonits M

更新日期：2018-11-15 00:00:00

abstract：:Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IAs) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes involved on lipid metabol...

journal_title：Molecular and cellular endocrinology

authors： Pereira MJ,Palming J,Rizell M,Aureliano M,Carvalho E,Svensson MK,Eriksson JW

更新日期：2013-01-30 00:00:00

abstract：:Interaction of tamoxifen, trioxifene and LY117018 with cytosol-estrogen receptors from immature rat uteri was compared. Determination of relative binding affinity (RBA) by competition with [3H] estradiol under various assay conditions revealed that the RBA of LY117018 increased with temperature while that of trioxifen...

journal_title：Molecular and cellular endocrinology

authors： Black LJ,Jones CD,Goode RL

更新日期：1981-04-01 00:00:00

abstract：:To identify cis-acting prolactin (PRL) response elements within the sheep beta-lactoglobulin (BLG) promoter, CHO cells were co-transfected with a rabbit PRL-receptor (PRL-R) expression plasmid and a number of BLG-CAT constructs. Resection through the 4200 bp BLG promoter diminished the PRL response. Mutation of the pr...

journal_title：Molecular and cellular endocrinology

authors： Demmer J,Burdon TG,Djiane J,Watson CJ,Clark AJ

更新日期：1995-01-01 00:00:00

abstract：:TRK oncogenes are observed in a consistent fraction of papillary thyroid carcinoma (PTC); they arise from the fusion of the 3' terminal sequences of the NTRK1/NGF receptor gene with 5' terminal sequences of various activating genes, such as TPM3, TPR and TFG. TRK oncoproteins display constitutive tyrosine-kinase activ...

journal_title：Molecular and cellular endocrinology

authors： Greco A,Miranda C,Pierotti MA

更新日期：2010-05-28 00:00:00

abstract：:The aim of this study is to further characterize the prolactin receptors (PRL-R) previously reported in the murine Leydig tumor MA-10 cell line, as well as to study their homologous and heterologous regulation. Two forms of PRL-R, a high and a low molecular weight form, were revealed by studies of covalent crosslinkin...

journal_title：Molecular and cellular endocrinology

authors： Weiss-Messer E,Ber R,Amit T,Barkey RJ

更新日期：1998-08-25 00:00:00

abstract：:Bone marrow-derived leukocytes are present in human endometrium/decidua and are believed to serve a variety of functions in this tissue. The number and type of leukocytes in endometrium/decidua vary with the hormonal milieu of the ovarian cycle, with blastocyst implantation, and during pregnancy. The factors that regu...

journal_title：Molecular and cellular endocrinology

authors： Arici A,MacDonald PC,Casey ML

更新日期：1995-02-01 00:00:00

abstract：:Primary cultures of ovine thyroid cells were induced to differentiate by addition of thyrotropin (TSH). This was demonstrated as an accumulation of 2 thyroid-specific proteins, thyroglobulin and thyroid peroxidase, using immunofluorescent staining methods and immunoprecipitation of biosynthetically labeled cultures. A...

journal_title：Molecular and cellular endocrinology

authors： Errick JE,Eggo MC,Burrow GN

更新日期：1985-11-01 00:00:00

abstract：:Exposure of the oestrogen-dominated rat myometrium to either isoproterenol or PGE2 resulted in a rapid but transient accumulation of cyclic AMP, with a progressive loss of responsiveness to the corresponding agonist. Induction of refractoriness was a time- and dose-related phenomenon. In the earliest time, desensitiza...

journal_title：Molecular and cellular endocrinology

authors： Tougui Z,Do Khac L,Harbon S

更新日期：1980-10-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a main factor promoting neovascularization (angiogenesis) of solid tumours as prostate carcinoma. Hypoxia stimulates VEGF gene expression by activating the hypoxia-inducible factor-1 (HIF-1alpha). In the present study, the hypoxia-mimicking agent Ni(2+) induced vasoactive i...

journal_title：Molecular and cellular endocrinology

authors： Collado B,Sánchez-Chapado M,Prieto JC,Carmena MJ

更新日期：2006-04-25 00:00:00

abstract：:Rat thyroid tissue and cultured rat thyrocyte lines contain two thyroglobulin (Tg) mRNAs: a 9 kb rTg-1 mRNA encoding the 330, kDa Tg monomer and a recently described 0.95 kb rTg-2 mRNA. These transcripts have identical 5' coding sequences (641 nucleotides); however, the 3' end of rTg-2 is comprised of coding and non-c...

journal_title：Molecular and cellular endocrinology

authors： Graves PN,Davies TF

更新日期：1993-06-01 00:00:00

abstract：:Aldosterone and cortisol are the major mineralocorticoid and glucocorticoid produced by the human adrenal. Circulating levels of angiotensin II and potassium control the adrenal production of aldosterone, while the production of cortisol is controlled mainly by adrenocorticotropin. The capacity of the adrenal cortex t...

journal_title：Molecular and cellular endocrinology

authors： Rainey WE

更新日期：1999-05-25 00:00:00

abstract：BACKGROUND:Mono-2-ethylhexyl phthalate (MEHP), an important metabolite of di (2-ethylhexyl) phthalate (DEHP), can induce lipid metabolic disorder. Previous studies have shown that MEHP promotes 3T3-L1 cell differentiation; however, the underlying mechanism is unclear. The present study was performed to investigate the ...

journal_title：Molecular and cellular endocrinology

authors： Qi W,Zhou L,Zhao T,Ding S,Xu Q,Han X,Zhao Y,Song X,Zhao T,Zhang X,Ye L

更新日期：2019-03-15 00:00:00

abstract：:Growth hormone (GH) and its mediator, the insulin-like growth factor-1 (IGF-1) regulate somatic growth, metabolism and many aspects of aging. As such, actions of GH/IGF have been studied in many tissues and organs over decades. GH and IGF-1 are part of the hypothalamic/pituitary somatotrophic axis that consists of man...

journal_title：Molecular and cellular endocrinology

authors： Dixit M,Poudel SB,Yakar S

更新日期：2021-01-01 00:00:00

abstract：:Non-alcoholic fatty liver disease (NAFLD) is associated with hepatic insulin resistance with the molecular basis of this association being not well understood. Here we studied the effect of hepatic triglyceride accumulation induced by postprandial triglyceride-rich lipoproteins (TGRL) on hepatic insulin sensitivity in...

journal_title：Molecular and cellular endocrinology

authors： Tatarczyk T,Ciardi C,Niederwanger A,Kranebitter M,Patsch JR,Pedrini MT

更新日期：2011-08-22 00:00:00

abstract：:Pituitary adenomas (PAs) hypersecrete hormones or cause mass effect symptoms, with 10%-35% patients showing resistance to standard therapies. Targeting epidermal growth factor receptor (EGFR) has significantly improved the clinical outcome in many cancers. In this study, immunochemistry results showed that EGFR associ...

journal_title：Molecular and cellular endocrinology

authors： Wei D,Yu Z,Cheng Y,Jiawei H,Jian G,Hua G,Guilan D

更新日期：2021-01-15 00:00:00

abstract：:The conversion of 3 beta-hydroxy-5-ene steroids by the enzyme complex 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) is an obligatory step in the biosynthesis of all classes of hormonal steroids in classical steroidogenic as well as in peripheral tissues. To develop a model more closely rel...

journal_title：Molecular and cellular endocrinology

authors： Simard J,Melner MH,Breton N,Low KG,Zhao HF,Periman LM,Labrie F

更新日期：1991-02-01 00:00:00

abstract：:The conversion of androgens to estrogens is catalyzed by a complex of enzymes that includes a specific cytochrome P-450 aromatase (P-450arom). In this paper we describe the high level expression of aromatase activity in the rat Leydig cell tumor line, R2C. We also report the isolation of cDNA clones encoding the rat a...

journal_title：Molecular and cellular endocrinology

authors： Lephart ED,Peterson KG,Noble JF,George FW,McPhaul MJ

更新日期：1990-03-26 00:00:00