The proximal milk protein binding factor binding site is required for the prolactin responsiveness of the sheep beta-lactoglobulin promoter in Chinese hamster ovary cells.

abstract：:We have examined the relationship between extracellular Ca2+, cytosolic free Ca2+ and insulin release in the clonal beta-cell line HIT-T15. Glucose-stimulated insulin release was dependent on the extracellular Ca2+ concentration in a dose-related manner; the threshold medium Ca2+ concentration for glucose-stimulated i...

journal_title：Molecular and cellular endocrinology

authors： Hughes SJ,Chalk JG,Ashcroft SJ

更新日期：1989-08-01 00:00:00

abstract：:In an effort to identify the signal transduction mechanism associated with the inhibition of juvenile hormone (JH) biosynthesis by the neuropeptides allatostatins, levels of the cyclic nucleotides cAMP and cGMP were measured in corpora allata (CA) of virgin and mated Diploptera punctata females using radioimmunoassays...

journal_title：Molecular and cellular endocrinology

authors： Cusson M,Yagi KJ,Guan XC,Tobe SS

更新日期：1992-11-01 00:00:00

abstract：:Northern blot analysis of total RNA from a variety of rabbit tissues indicated that placenta is the primary site of expression of the protein hormone relaxin (previously called SQ10) in rabbits. Relaxin was not detected by this method in other rabbit tissues, including normal trachea and several squamous tissues. Howe...

journal_title：Molecular and cellular endocrinology

authors： Bernacki SH,Medvedev A,Holloway G,Dawson M,Lotan R,Jetten AM

更新日期：1998-03-16 00:00:00

abstract：:Activating mutations in the follicle-stimulating hormone (FSH) receptor (FSHR) gene are rarely detected due to the absence of a clearly defined phenotype, particularly in men. We here report the biochemical features of a novel mutation in the first extracellular loop of the FSHR. The mutation (N431I) was detected in a...

journal_title：Molecular and cellular endocrinology

authors： Casas-González P,Scaglia HE,Pérez-Solís MA,Durand G,Scaglia J,Zariñán T,Dias JA,Reiter E,Ulloa-Aguirre A

更新日期：2012-11-25 00:00:00

abstract：:Pituitary cultures from adult rats contain two subtypes of prolactin (PRL) cells, small-plaque (SP) and large-plaque (LP) lactotropes, which exhibit distinct rates of basal secretion and thereby form PRL plaques of different sizes in reverse hemolytic plaque assay experiments. In the present study, we have used plaque...

journal_title：Molecular and cellular endocrinology

authors： Horta J,Cota G

更新日期：1993-04-01 00:00:00

abstract：:Oxytocin receptors (OT-R) are known to be involved in the course of labor since a massive increase in OT-binding sites is observed in the uterus just before parturition. Vasopressin (AVP)-binding sites have also been observed and have been shown to mediate uterotonic responses. To determine exactly which subtypes of O...

journal_title：Molecular and cellular endocrinology

authors： Clerget MS,Elalouf JM,Germain G

更新日期：1997-12-31 00:00:00

abstract：:We previously described an androgen receptor (AR) point mutation located in the DNA-binding domain (DBD), adjacent to another AR substitution. Both were observed in two unrelated families with male breast cancer (MBC) and partial androgen insensitivity syndrome. This work was designed to determine the potential role o...

journal_title：Molecular and cellular endocrinology

authors： Poujol N,Lobaccaro JM,Chiche L,Lumbroso S,Sultan C

更新日期：1997-06-20 00:00:00

abstract：:Chronic restraint stress (CRS) magnifies restraint-induced corticosterone secretion through a mechanism involving increased adrenocortical 5-HT content and turnover. We analysed the impact of CRS on serotonin transporter (SERT) expression and distribution in rat adrenal glands. Male Wistar rats were submitted to CRS (...

journal_title：Molecular and cellular endocrinology

authors： Shanker S,Saroj N,Cordova EJ,Jarillo-Luna RA,López-Sánchez P,Terrón JA

更新日期：2020-12-01 00:00:00

abstract：:Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, ...

journal_title：Molecular and cellular endocrinology

authors： Vota D,Aguero M,Grasso E,Hauk V,Gallino L,Soczewski E,Pérez Leirós C,Ramhorst R

更新日期：2017-03-05 00:00:00

abstract：:Galectin-1 is highly expressed in blastocysts and trophoblast giant cells during implantation, and dysregulated galectin-1 is associated with many pregnancy-related abnormalities. Elevated galectin-1 contributes to cancer cells invasion. Here, we found that galectin-1 is expressed in mouse oocytes, preimplantation emb...

journal_title：Molecular and cellular endocrinology

authors： You JL,Wang W,Tang MY,Ye YH,Liu AX,Zhu YM

更新日期：2018-07-15 00:00:00

abstract：:Corticotropin-releasing factor (CRF) receptor type 1 (CRF(1) receptor) mRNA levels are down-regulated by CRF via the cyclic AMP-protein kinase A (PKA) pathway. In this study, we focused on the involvement of both the mitogen-activated protein (MAP) kinase pathway and PKA in this regulation. Real-time PCR (RT-PCR) reve...

journal_title：Molecular and cellular endocrinology

authors： Moriyama T,Kageyama K,Kasagi Y,Iwasaki Y,Nigawara T,Sakihara S,Suda T

更新日期：2005-11-24 00:00:00

abstract：:We tested hamster uterine progesterone receptor (Rp) forms for binding to different chromatin preparations. Similar forms of chick oviduct Rp were used for comparison. Hamster Rp elutes from DEAE-Sephacel in the two peaks, peak I at 115 mM KCl and peak II at 205 mM KCl. Chick Rp peaks I and II elute at 125 mM and 300 ...

journal_title：Molecular and cellular endocrinology

authors： Cobb AD,Leavitt WW

更新日期：1987-07-01 00:00:00

abstract：:The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) protects the non-discriminating mineralocorticoid receptor from occupation by glucocorticoids. In man the enzyme is also highly expressed in the placenta where it is thought to also protect the fetus from the high circulating levels of maternal glu...

journal_title：Molecular and cellular endocrinology

authors： Li KX,Smith RE,Ferrari P,Funder JW,Krozowski ZS

更新日期：1996-06-18 00:00:00

abstract：:Digital video imaging indicated that about 80% of fura-2-loaded single human thyroid cells responded to TSH, resulting in an increase in intracellular Ca2+ concentration ([Ca2+]i). Most of the TSH-sensitive cells further responded to N6-(L-2-phenylisopropyl)-adenosine (PIA) showing a transient [Ca2+]i rise in a PIA do...

journal_title：Molecular and cellular endocrinology

authors： Yanagita Y,Okajima F,Sho K,Nagamachi Y,Kondo Y

更新日期：1996-04-19 00:00:00

abstract：:The requirement for caspases (ICE-like proteases) were investigated in mediating apoptosis of WEHI7.2 mouse lymphoma cells in response to two death inducers with different mechanisms of action, the glucocorticoid hormone dexamethasone (DX) and the calcium-ATPase inhibitor thapsigargin (TG). Apoptosis induction by thes...

journal_title：Molecular and cellular endocrinology

authors： McColl KS,He H,Zhong H,Whitacre CM,Berger NA,Distelhorst CW

更新日期：1998-04-30 00:00:00

abstract：:Micropipettes were used to record electrical activity from single neurons in hypothalamic tissue slices, in the hypothalamic arcuate nucleus (ARC), and in the periventricular and suprachiasmatic preoptic nuclei (POA). Responses were measured following in vitro application of luteinizing hormone releasing hormone (LHRH...

journal_title：Molecular and cellular endocrinology

authors： Pan JT,Kow LM,Kendall DA,Kaiser ET,Pfaff DW

更新日期：1986-12-01 00:00:00

abstract：:Studies on the age-related decline of growth hormone (GH) release have ignored that the population of GH-producing cells (somatotropes) is heterogeneous. In aging male rats, centrifugation of dispersed pituitary cells in a density gradient yields two somatotrope subpopulations, i.e. low- (LD) and high-density (HD) cel...

journal_title：Molecular and cellular endocrinology

authors： Dobado-Berrios PM,Ruiz-Navarro A,Lopez-Pedrera R,Gonzalez de Aguilar JL,Torronteras R,Hidalgo-Diaz C,Gracia-Navarro F

更新日期：1996-10-30 00:00:00

abstract：:Glucocorticoids play a major role in attenuation of the inflammatory response. These steroid hormones are able to induce apoptosis in cells of the hematopoietic system such as monocytes, macrophages and T-lymphocytes that are involved in the inflammation reaction. In contrast, it was discovered recently that in glandu...

journal_title：Molecular and cellular endocrinology

authors： Amsterdam A,Sasson R

更新日期：2002-03-28 00:00:00

abstract：:The canonical renin-angiotensin system (RAS) involves the initial action of renin to cleave angiotensinogen to angiotensin I (ANG I), which is then converted to ANG II by the angiotensin converting enzyme (ACE). ANG II plays a critical role in numerous physiological functions, and RAS overactivity underlies many condi...

journal_title：Molecular and cellular endocrinology

authors： Henriksen EJ,Prasannarong M

更新日期：2013-09-25 00:00:00

abstract：:Ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of Y-1 mouse adrenal and bovine fasciculata cells. The activity is inhibited by three inhibitors of calmodulin (trifluoperazine, W-7 and pimozide) with EC50 in the low micromolar range. Protein phosphatase activity is inhibited by vanadat...

journal_title：Molecular and cellular endocrinology

authors： Papadopoulos V,Brown AS,Hall PF

更新日期：1989-05-01 00:00:00

abstract：:Increased production of inflammatory cytokines is suggested to be of importance for initiation and progression of insulin resistance. Chronic treatment with the AMP analogue 5-aminoimidazole-4-carboxamide-1-beta-d-ribonucleoside (AICAR) has been shown to improve insulin sensitivity and prevent the development of diabe...

journal_title：Molecular and cellular endocrinology

authors： Lihn AS,Pedersen SB,Lund S,Richelsen B

更新日期：2008-09-24 00:00:00

abstract：:The NAD+ dependent (K or type 2) isozyme of 11 beta-hydroxysteroid dehydrogenase oxidizes glucocorticoids and thus prevents them from occupying mineralocorticoid receptors. Mutations in the HSD11K (HSD11B2) gene encoding this isozyme cause a genetic form of hypertension, the syndrome of apparent mineralocorticoid exce...

journal_title：Molecular and cellular endocrinology

authors： Agarwal AK,White PC

更新日期：1996-07-23 00:00:00

abstract：:We have designed and synthesized a biotinylated vasopressin antagonist which is a selective probe for studying the V1a subtype of vasopressin receptor. Initially we synthesized the novel vasopressin analogue d(CH2)5Tyr(Me)2LysNH2(9)AVP (ALVP). Biotinamidocaproate was subsequently coupled to the epsilon-amino group of ...

journal_title：Molecular and cellular endocrinology

authors： Howl J,Kerr ID,Chan CH,Wheatley M

更新日期：1991-05-01 00:00:00

abstract：:The ability of angiotensin-II (A-II) to increase cAMP production in adrenocortical cells is not widely accepted due to numerous conflicting reports. The recent observation that rat adrenal cells exhibit multiple subtypes of A-II receptors raises the possibility that a specific subtype could be responsible for controll...

journal_title：Molecular and cellular endocrinology

authors： Rainey WE,Byrd EW,Sinnokrot RA,Carr BR

更新日期：1991-10-01 00:00:00

abstract：:In order to study the distribution of LH (HCG) receptors on luteal cells ferritin was coupled to ovine LH with glutaraldehyde and purified by gel chromatography. The conjugate (FELH) competed with 125I-hCG for binding to isolated luteal membranes and stimulated a dose-dependent release of progesterone (P) from isolate...

journal_title：Molecular and cellular endocrinology

authors： Luborsky JL,Behrman HR

更新日期：1979-08-01 00:00:00

abstract：:Secreted, plasma membrane, and resident proteins of the secretory pathway are synthesized in the endoplasmic reticulum (ER) where they undergo post-translational modifications, oxidative folding, and subunit assembly in tightly monitored processes. An ER quality control (ERQC) system oversees protein maturation and en...

journal_title：Molecular and cellular endocrinology

authors： Oikonomou C,Hendershot LM

更新日期：2020-01-15 00:00:00

abstract：:In man, two isoforms of growth hormone receptor (GHR) have been reported. The first, hGHR corresponds to the original form described by Leung et al. (J. Biol. Chem. 264 (1987) 9905-9910). The second, hGHRd3, missing 22 N-terminal amino acids is encoded by an mRNA derived from alternative splicing and deletion of the t...

journal_title：Molecular and cellular endocrinology

authors： Esposito N,Paterlini P,Kelly PA,Postel-Vinay MC,Finidori J

更新日期：1994-07-01 00:00:00

abstract：:cAMP signalling plays a key role in the normal physiology of the pituitary gland, regulating cellular growth and proliferation, hormone production and release. Deregulation of the cAMP signalling pathway has been reported to be a common occurrence in pituitary tumorigenesis. Several mechanisms have been implicated inc...

journal_title：Molecular and cellular endocrinology

authors： Formosa R,Vassallo J

更新日期：2014-07-05 00:00:00

abstract：:Conjugated linoleic acid (CLA) supplementation has been reported to induce insulin resistance in animals and humans, however, the underlying mechanisms remain unclear. The aim of this study was to examine the direct effects of CLA on leptin and adiponectin secretion, two hormones with actions known to influence insuli...

journal_title：Molecular and cellular endocrinology

authors： Pérez-Matute P,Marti A,Martínez JA,Fernández-Otero MP,Stanhope KL,Havel PJ,Moreno-Aliaga MJ

更新日期：2007-03-30 00:00:00

abstract：:Cell-based studies previously showed that the ATP-binding cassette transporter A1 (ABCA1) transfers cholesterol across mammary epithelial cells (MEC). Data for phospholipid transport are lacking, and it is unclear from which cellular source the transported cholesterol stems, whether this transport activates signaling ...

journal_title：Molecular and cellular endocrinology

authors： Ontsouka CE,Huang X,Aliyev E,Albrecht C

更新日期：2017-01-05 00:00:00