Abstract:
:Increased production of inflammatory cytokines is suggested to be of importance for initiation and progression of insulin resistance. Chronic treatment with the AMP analogue 5-aminoimidazole-4-carboxamide-1-beta-d-ribonucleoside (AICAR) has been shown to improve insulin sensitivity and prevent the development of diabetes in rodents. We investigated the effects of AICAR on the expression and production of inflammatory cytokines from human adipose tissue and skeletal muscle cells as well as intact rat skeletal muscles in vitro. AICAR dose-dependently decreased interleukin-6 (IL-6) gene expression and secretion in human adipose tissue and in human skeletal muscle cells. In parallel, AICAR inhibited interleukin-8 (IL-8) secretion in human adipose tissue and skeletal muscle cells, as well as reduced IL-8 gene expression in skeletal muscle cells. In intact rat extensor digitorum longus (EDL) muscle fibres AICAR markedly decreased IL-6 and IL-8 gene expression. In conclusion, AICAR inhibits the production of IL-6 and IL-8 human adipose tissue and in skeletal muscle cells. We suggest that decreased cytokine production might play a role for the AICAR-induced increase in insulin sensitivity.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Lihn AS,Pedersen SB,Lund S,Richelsen Bdoi
10.1016/j.mce.2008.06.004subject
Has Abstractpub_date
2008-09-24 00:00:00pages
36-41issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(08)00273-6journal_volume
292pub_type
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