Abstract:
:We investigated the involvement of protein synthesis in the stimulatory action of thyroid hormones on amino acid accumulation and characterized K(+) currents involved in the hyperpolarizing effect of thyroxine (T(4)) on Sertoli cells. Immature rat testes were incubated in Krebs Ringer-bicarbonate buffer (KRb) in the presence of [(14)C]methylaminoisobutyric acid with and without T(4), 3,5,3'-l-triiodothyronine (T(3)) and/or cycloheximide. Sertoli cells were monitored by intracellular recording in a chamber perfused with KRb with and without T(4), T(3) and/or blockers, and the membrane potential was monitored. T(4) and T(3) stimulated amino acid accumulation and protein synthesis. Treatment with cycloheximide diminished T(3) stimulatory actions on amino acid accumulation but had no effect on T(4) action. Both hormones elicited a hyperpolarization of the Sertoli cell membrane potential which involved K(+) channels, since TEA and apamin abolished this effect. These findings on rapid membrane actions of thyroid hormone in the testis suggest that some effects of T(4) are modulated by non-genomic mechanisms.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Menegaz D,Zamoner A,Royer C,Leite LD,Bortolotto ZA,Silva FRdoi
10.1016/j.mce.2005.11.019keywords:
subject
Has Abstractpub_date
2006-02-26 00:00:00pages
128-34issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(05)00424-7journal_volume
246pub_type
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