Rapid responses to thyroxine in the testis: active protein synthesis-independent pathway.

Abstract:

:We investigated the involvement of protein synthesis in the stimulatory action of thyroid hormones on amino acid accumulation and characterized K(+) currents involved in the hyperpolarizing effect of thyroxine (T(4)) on Sertoli cells. Immature rat testes were incubated in Krebs Ringer-bicarbonate buffer (KRb) in the presence of [(14)C]methylaminoisobutyric acid with and without T(4), 3,5,3'-l-triiodothyronine (T(3)) and/or cycloheximide. Sertoli cells were monitored by intracellular recording in a chamber perfused with KRb with and without T(4), T(3) and/or blockers, and the membrane potential was monitored. T(4) and T(3) stimulated amino acid accumulation and protein synthesis. Treatment with cycloheximide diminished T(3) stimulatory actions on amino acid accumulation but had no effect on T(4) action. Both hormones elicited a hyperpolarization of the Sertoli cell membrane potential which involved K(+) channels, since TEA and apamin abolished this effect. These findings on rapid membrane actions of thyroid hormone in the testis suggest that some effects of T(4) are modulated by non-genomic mechanisms.

journal_name

Mol Cell Endocrinol

authors

Menegaz D,Zamoner A,Royer C,Leite LD,Bortolotto ZA,Silva FR

doi

10.1016/j.mce.2005.11.019

keywords:

subject

Has Abstract

pub_date

2006-02-26 00:00:00

pages

128-34

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

S0303-7207(05)00424-7

journal_volume

246

pub_type

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