Expression of two isoforms of the human growth hormone receptor in normal liver and hepatocarcinoma.

Abstract:

:In man, two isoforms of growth hormone receptor (GHR) have been reported. The first, hGHR corresponds to the original form described by Leung et al. (J. Biol. Chem. 264 (1987) 9905-9910). The second, hGHRd3, missing 22 N-terminal amino acids is encoded by an mRNA derived from alternative splicing and deletion of the third exon. This latter form has been isolated from placenta and its tissue distribution, as well its function warrant further examination. We studied the relative expression of the two isoforms of GHR in liver at various stages of development of differentiation. Several samples from fetal livers and hepatocarcinomas were analysed and we observed that hGHR and hGHRd3 can be expressed solely or coordinately. Furthermore, we investigated the possibility that alternative splicing of exon 3 could be related to the specific use of different promoters. Our data show that both isoforms have identical 5'-untranslated regions in normal liver or in hepatoma cell lines. Finally, these experiments strongly suggest that hGHRd3 is efficiently translated as a functional receptor, with the soluble form of GHR being recovered in the cell media. The results of the present study support the concept that both isoforms are expressed in human liver, with exon 3 alternative splicing being neither tissue specific nor related to hepatic differentiation.

journal_name

Mol Cell Endocrinol

authors

Esposito N,Paterlini P,Kelly PA,Postel-Vinay MC,Finidori J

doi

10.1016/0303-7207(94)90064-7

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

13-20

issue

1-2

eissn

0303-7207

issn

1872-8057

pii

0303-7207(94)90064-7

journal_volume

103

pub_type

杂志文章
  • Differences in B7 and CD28 family gene expression in the peripheral blood between newly diagnosed young-onset and adult-onset type 1 diabetes patients.

    abstract::Type-1 diabetes (T1D) is a heterogeneous autoimmune disease, and there are pathogenetic differences between young- and adult-onset T1D patients. We hypothesized that the expressions of genes involved in costimulatory immune system pathways in peripheral blood are differently regulated in young- and adult-onset T1D. St...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2015.05.012

    authors: Pruul K,Kisand K,Alnek K,Metsküla K,Reimand K,Heilman K,Peet A,Varik K,Peetsalu M,Einberg Ü,Tillmann V,Uibo R

    更新日期:2015-09-05 00:00:00

  • Regulation of the secretion of proteins synthesized by the human breast cancer cell line, MCF-7.

    abstract::In two sublines of the estrogen receptor-positive human breast cancer cell line, MCF-7, propagated with 0.5% fetal calf serum (FCS) (subline 0.5) and without serum (subline 9), respectively, addition of 10% newborn calf serum (NCS) inhibits cell proliferation and stimulates the secretion of a 42 kDa protein. Both with...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(89)90016-6

    authors: Lykkesfeldt AE,Laursen I,Briand P

    更新日期:1989-04-01 00:00:00

  • The circadian clock and glucocorticoids--interactions across many time scales.

    abstract::Glucocorticoids are steroid hormones of the adrenal gland that are an integral component of the stress response and regulate many physiological processes, including metabolism and immune response. Their release into the blood is highly dynamic and occurs in about hourly pulses, the amplitude of which is modulated in a...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/j.mce.2013.05.012

    authors: Dickmeis T,Weger BD,Weger M

    更新日期:2013-11-05 00:00:00

  • Smad5 regulates Akt2 expression and insulin-induced glucose uptake in L6 myotubes.

    abstract::Insulin-induced glucose uptake by skeletal muscle results from Akt2 activation and is severely impaired during insulin resistance. Recently, we and others have demonstrated that BMP9 improves glucose homeostasis in diabetic and non-diabetic rodents. However, the mechanism by which BMP9 modulates insulin action remains...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2010.01.003

    authors: Anhê FF,Lellis-Santos C,Leite AR,Hirabara SM,Boschero AC,Curi R,Anhê GF,Bordin S

    更新日期:2010-05-05 00:00:00

  • Roles of gonadotropins and meiosis-activating sterols in meiotic resumption of cultured follicle-enclosed mouse oocytes.

    abstract::This study describes a model for short-term culture of intact mouse follicles under serum-free conditions. Follicles were either obtained from immature mice receiving no ovarian stimulation (i.e. no eCG-primed protocol, group I) or from mice undergoing ovarian stimulation (i.e. eCG-primed protocol, group II). Follicle...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2003.11.030

    authors: Xie H,Xia G,Byskov AG,Andersen CY,Bo S,Tao Y

    更新日期:2004-04-15 00:00:00

  • Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome.

    abstract::Melatonin can be synthesized and secreted not only by the pineal gland but also by many extra-pineal tissues. It has been shown that many ovarian functions are regulated by melatonin locally. Ovarian hyperstimulation syndrome (OHSS) is a serious complication during ovulation induction of the in vitro fertilization tre...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2020.110981

    authors: Li Y,Fang L,Zhang R,Wang S,Li Y,Yan Y,Yu Y,Cheng JC,Sun YP

    更新日期:2020-12-01 00:00:00

  • The oxytocin receptor antagonist, Atosiban, activates pro-inflammatory pathways in human amnion via G(αi) signalling.

    abstract::Oxytocin (OT) plays an important role in the onset of human labour by stimulating uterine contractions and promoting prostaglandin/inflammatory cytokine synthesis in amnion via oxytocin receptor (OTR) coupling. The OTR-antagonist, Atosiban, is widely used as a tocolytic for the management of acute preterm labour. We f...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2015.11.012

    authors: Kim SH,MacIntyre DA,Hanyaloglu AC,Blanks AM,Thornton S,Bennett PR,Terzidou V

    更新日期:2016-01-15 00:00:00

  • Role of epididymal receptor HE6 in the regulation of sperm microenvironment.

    abstract::HE6 (GPR64) is a highly conserved, tissue-specific heptahelical receptor of the human epididymis. The seven transmembrane (TM7) domains are a hallmark of G-protein-coupled receptors (GPCRs) which have a proven history of being excellent therapeutic targets. Of all currently marketed drugs, >30% are modulators of speci...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/j.mce.2005.12.023

    authors: Kirchhoff C,Obermann H,Behnen M,Davies B

    更新日期:2006-05-16 00:00:00

  • Phorbol ester- and luteinizing hormone-induced phosphorylation of membrane proteins in bovine luteal cells.

    abstract::In this study we have investigated the protein phosphorylation pattern in the membrane fraction prepared from bovine luteal cells. The phosphorylation reaction was carried out in vitro, under defined conditions, using either [gamma-32P]ATP or [gamma-35S]ATP as the phosphate donor. The results obtained show that [gamma...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(90)90018-4

    authors: Budnik LT,Mukhopadhyay AK

    更新日期:1990-03-05 00:00:00

  • Male infertility, genetic analysis of the DAZ genes on the human Y chromosome and genetic analysis of DNA repair.

    abstract::Many genes that are required for fertility have been identified in model organisms (). Mutations in these genes cause infertility due to defects in development of the germ cell lineage, but the organism is otherwise healthy. Although human reproduction is undoubtedly as complex as that of other organisms, very few fer...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/s0303-7207(01)00646-3

    authors: Fox MS,Reijo Pera RA

    更新日期:2001-11-26 00:00:00

  • Gonadotropins and inhibins along the development of a luteinized rat ovarian tumor.

    abstract::Luteinized intrasplenic ovarian tumors develop in response to high circulating gonadotropins. The relationship between tumor development, gonadotropins and inhibins was studied. Tumor-bearing animals were sacrificed weekly along the first 6 weeks of development. Inhibins were measured by enzyme-linked immunosorbent as...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/s0303-7207(03)00085-6

    authors: Hockl P,Sorianello E,Chamson-Reig A,Ballerini G,Campo S,Groome N,Lux-Lantos V,Libertun C

    更新日期:2003-05-30 00:00:00

  • Effect of transforming growth factor-beta 1 on the insulin-like growth factor system in cultured porcine Leydig cells.

    abstract::Using as a model system, primary cultures of porcine Leydig cells, we have shown that transforming growth factor-beta 1 (TGF-beta 1) (2 ng/ml, 72 h) antagonizes the stimulatory action of insulin-like growth factor-I (IGF-I) on luteinizing hormone (LH/hCG) receptors. We therefore investigated the action of TGF-beta 1 o...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(94)90015-9

    authors: Besset V,Collette J,Chauvin MA,Franchimont P,Benahmed M

    更新日期:1994-03-01 00:00:00

  • The link between fibroblast growth factor 21 and sterol regulatory element binding protein 1c during lipogenesis in hepatocytes.

    abstract::Recently fibroblast growth factor 21 (FGF21) has been identified as a potent regulator in glucose and lipid homeostasis. Here, we firstly investigated the metabolic role of FGF21 in human liver-derived HepG2 cells, and suggested that overexpression of FGF21 suppressed triglyceride accumulation by inhibiting the transc...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2011.05.003

    authors: Zhang Y,Lei T,Huang JF,Wang SB,Zhou LL,Yang ZQ,Chen XD

    更新日期:2011-08-06 00:00:00

  • Lactogenic hormones and extracellular matrix regulate expression of IGF-1 linked to MMTV-LTR in mammary epithelial cells.

    abstract::The cell line MD-IGF-1, containing an ovine IGF-1 cDNA driven by the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promoter, was used to study expression of IGF-1 linked to the MMTV-LTR in bovine mammary epithelial cells in response to various hormonal and substratum stimuli. Acute sensitivity of the MMTV-...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(93)90105-s

    authors: Romagnolo D,Akers RM,Wong EA,Boyle PL,McFadden TB,Byatt JC,Turner JD

    更新日期:1993-10-01 00:00:00

  • Induction of hepatic glycogen synthesis by glucocorticoids is not mediated by insulin.

    abstract::Administration of 0.1 or 1 mg of prednisolone to fed mice caused a 5-fold activation of glycogen synthase in the liver after 3h, without significant changes in the circulating levels of glucose or insulin, or the hepatic concentration of cyclic AMP. Adrenalectomized fasted rats responded to cortisol (10 mg) with an in...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(82)90067-3

    authors: Vanstapel F,Bollen M,de Wulf H,Stalmans W

    更新日期:1982-06-01 00:00:00

  • Genomic organization and promoter analysis of mouse follistatin-related gene (FLRG).

    abstract::Follistatin (FS) is well characterized as an activin-binding protein. Recently, a novel follistatin-like protein called follistatin-related gene (FLRG) that has a similar domain organization to that of follistatin has been identified. Like follistatins, FLRG binds activins and bone morphogenetic proteins (BMPs). To st...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/s0303-7207(01)00734-1

    authors: Nakatani M,Yamakawa N,Matsuzaki T,Shimasaki S,Sugino H,Tsuchida K

    更新日期:2002-03-28 00:00:00

  • Endocrine actions of vitamin D in skin: Relevance for photocarcinogenesis of non-melanoma skin cancer, and beyond.

    abstract::The skin represents a pivotal organ for the human body's vitamin D endocrine system, being both the site of ultraviolet (UV)-B-induced vitamin D synthesis and a target tissue for the pluripotent effects of 1,25(OH)2D3 and other biologically active vitamin D metabolites. As many other steroid hormones, 1,25(OH)2D3 exer...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/j.mce.2017.05.001

    authors: Reichrath J,Saternus R,Vogt T

    更新日期:2017-09-15 00:00:00

  • Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.

    abstract::1,25-Dihydroxyvitamin D(3) (1,25D(3)), the active metabolite of vitamin D(3), inhibits breast cancer cell growth in vivo and in vitro. To examine mechanisms of 1,25D(3) induced growth arrest and apoptosis, cell lines were established from DMBA induced mammary tumors derived from vitamin D(3) receptor knockout (VDRKO) ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/s0303-7207(02)00416-1

    authors: Zinser GM,McEleney K,Welsh J

    更新日期:2003-02-28 00:00:00

  • Role of the androgen receptor in urothelial cancer.

    abstract::Men have had a substantially higher risk of developing bladder cancer than women. This has prompted research on androgen-mediated androgen receptor (AR) signaling in urothelial cancer. Indeed, increasing preclinical evidence indicates that AR activation correlates with the promotion of urothelial carcinogenesis and tu...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/j.mce.2017.06.021

    authors: Inoue S,Mizushima T,Miyamoto H

    更新日期:2018-04-15 00:00:00

  • Na+/H+ exchange is responsible for intracellular pH regulation in insulin-secreting HIT-T15 cells.

    abstract::The addition of glucose to suspensions of HIT-T15 insulinoma cells caused a small, transient acidification followed by a gradual, progressive alkalinisation, as assessed by the fluorescent pH-sensitive dye 2',7'-biscarboxyethyl-5'-(6')-carboxyfluorescein (BCECF). Treatment of cells with acetate or lactate produced an ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(90)90070-o

    authors: Trebilcock R,Lynch A,Tomlinson S,Best L

    更新日期:1990-05-28 00:00:00

  • The immunosuppressive agents rapamycin, cyclosporin A and tacrolimus increase lipolysis, inhibit lipid storage and alter expression of genes involved in lipid metabolism in human adipose tissue.

    abstract::Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IAs) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes involved on lipid metabol...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2012.10.030

    authors: Pereira MJ,Palming J,Rizell M,Aureliano M,Carvalho E,Svensson MK,Eriksson JW

    更新日期:2013-01-30 00:00:00

  • Desethylamiodarone is a competitive inhibitor of the binding of thyroid hormone to the thyroid hormone alpha 1-receptor protein.

    abstract::Desethylamiodarone (DEA), the major metabolite of the potent antiarrythmic drug amiodarone, is a non-competitive inhibitor of the binding of thyroid hormone (T3) to the beta 1-thyroid hormone receptor (T3R). In the present study, we investigated whether DEA acts in a similar way with respect to the alpha 1-T3R. The ch...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(95)03578-u

    authors: van Beeren HC,Bakker O,Wiersinga WM

    更新日期:1995-07-01 00:00:00

  • Activation of MAP kinase by the LHRH receptor through a dual mechanism involving protein kinase C and a pertussis toxin-sensitive G protein.

    abstract::The LHRH receptor in alpha T3-1 gonadotrope cells was shown to bring about a marked and sustained activation of MAP kinase. This response was prevented by protein kinase C inhibition or down-regulation and could be partially mimicked by phorbol ester. Additional evidence for inhibition of this response by pertussis to...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(95)03616-f

    authors: Sim PJ,Wolbers WB,Mitchell R

    更新日期:1995-08-11 00:00:00

  • Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

    abstract::The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were c...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/j.mce.2019.110506

    authors: Macedo TJS,Santos JMS,Bezerra MÉS,Menezes VG,Gouveia BB,Barbosa LMR,Lins TLBG,Monte APO,Barberino RS,Batista AM,Barros VRP,Wischral A,Queiroz MAA,Araújo GGL,Matos MHT

    更新日期:2019-09-15 00:00:00

  • Molecular endocrinology of female reproductive behavior.

    abstract::Epigenetic methodologies address mechanisms of estrogenic effects on hypothalamic and preoptic neurons, as well as mechanisms by which stress can interfere with female reproductive behaviors. Recent results are reviewed. ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章,评审

    doi:10.1016/j.mce.2017.10.019

    authors: Pfaff DW,Gagnidze K,Hunter RG

    更新日期:2018-05-15 00:00:00

  • A quantitative electron microscope autoradiographic study on [3H]prostaglandin E1 and F2 alpha internalization in bovine luteal slices.

    abstract::Very few silver grains were seen on the luteal cell surface and none intracellularly after incubation for 2 h at 4 degrees C with 10 nM [3H]prostaglandin (PG) E1 or [3H]PGF2 alpha. However, incubation at 22 degrees C or 38 degrees C for 2 h resulted in association of numerous grains on the luteal cell surface as well ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(84)90110-2

    authors: Chegini N,Rao CV,Cobbs G

    更新日期:1984-12-01 00:00:00

  • Thyroid hormone regulates expression of the thyrotropin beta-subunit gene from both transcription start sites in the mouse and rat.

    abstract::Thyroid hormones suppress transcription of the gene for the beta-subunit of thyrotropin (TSH beta). Since the TSH beta gene in both the mouse and the rat contains two start sites of transcription in exon 1, we have investigated whether expression of the gene from each start site is differentially regulated by thyroid ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(90)90024-3

    authors: Gurr JA,Januszeski MM,Tidikis IM,Norcross JJ,Kourides IA

    更新日期:1990-07-09 00:00:00

  • Regulation of steroidogenesis in crayfish molting glands: involvement of protein synthesis.

    abstract::The involvement of continuous protein synthesis in the mechanisms of crustacean steroidogenesis was investigated using crayfish molting glands (Y-organs). During intermolt, Y-organ steroidogenic activity is low. Eyestalk ablation initiates premolt which is characterized by a rapid increase in the production of ecdyste...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(95)03489-t

    authors: Dauphin-Villemant C,Böcking D,Sedlmeier D

    更新日期:1995-03-01 00:00:00

  • Chemotactic and mitogenic activities of granulosa cells in developing follicles.

    abstract::Chemotactic and mitogenic activities of granulosa cells in developing follicles were studied. Immature rats were subcutaneously injected with 20 IU of pregnant mare's serum gonadotrophin and killed at various intervals after injection. The ovaries were removed and granulosa cells were isolated and cultured in a serum-...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(92)90070-m

    authors: Lin MT,Wei SJ,Wing LY

    更新日期:1992-03-01 00:00:00

  • Immunocytochemical localization of prolactin receptors in rat liver cells: I. Dependence on sex and sex steroids.

    abstract::The peculiarities of cellular and tissue distribution of prolactin receptors (PRLR) in the liver of female and male rats with different sex steroid status were investigated in paraplast-embedded tissue with the indirect immunoperoxidase technique. Two clones of antibodies directed outside the PRL-binding site (U6) or ...

    journal_title:Molecular and cellular endocrinology

    pub_type: 杂志文章

    doi:10.1016/0303-7207(94)90037-x

    authors: Smirnova OV,Petraschuk OM,Kelly PA

    更新日期:1994-10-01 00:00:00