Abstract:
:In man, two isoforms of growth hormone receptor (GHR) have been reported. The first, hGHR corresponds to the original form described by Leung et al. (J. Biol. Chem. 264 (1987) 9905-9910). The second, hGHRd3, missing 22 N-terminal amino acids is encoded by an mRNA derived from alternative splicing and deletion of the third exon. This latter form has been isolated from placenta and its tissue distribution, as well its function warrant further examination. We studied the relative expression of the two isoforms of GHR in liver at various stages of development of differentiation. Several samples from fetal livers and hepatocarcinomas were analysed and we observed that hGHR and hGHRd3 can be expressed solely or coordinately. Furthermore, we investigated the possibility that alternative splicing of exon 3 could be related to the specific use of different promoters. Our data show that both isoforms have identical 5'-untranslated regions in normal liver or in hepatoma cell lines. Finally, these experiments strongly suggest that hGHRd3 is efficiently translated as a functional receptor, with the soluble form of GHR being recovered in the cell media. The results of the present study support the concept that both isoforms are expressed in human liver, with exon 3 alternative splicing being neither tissue specific nor related to hepatic differentiation.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Esposito N,Paterlini P,Kelly PA,Postel-Vinay MC,Finidori Jdoi
10.1016/0303-7207(94)90064-7subject
Has Abstractpub_date
1994-07-01 00:00:00pages
13-20issue
1-2eissn
0303-7207issn
1872-8057pii
0303-7207(94)90064-7journal_volume
103pub_type
杂志文章abstract::Type-1 diabetes (T1D) is a heterogeneous autoimmune disease, and there are pathogenetic differences between young- and adult-onset T1D patients. We hypothesized that the expressions of genes involved in costimulatory immune system pathways in peripheral blood are differently regulated in young- and adult-onset T1D. St...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-08-06 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-03-28 00:00:00
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更新日期:2017-09-15 00:00:00
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journal_title:Molecular and cellular endocrinology
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更新日期:2013-01-30 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1995-08-11 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:2019-09-15 00:00:00
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更新日期:2018-05-15 00:00:00
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pub_type: 杂志文章
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更新日期:1984-12-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(90)90024-3
更新日期:1990-07-09 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(92)90070-m
更新日期:1992-03-01 00:00:00
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
doi:10.1016/0303-7207(94)90037-x
更新日期:1994-10-01 00:00:00