Abstract：
:Rat thyroid tissue and cultured rat thyrocyte lines contain two thyroglobulin (Tg) mRNAs: a 9 kb rTg-1 mRNA encoding the 330, kDa Tg monomer and a recently described 0.95 kb rTg-2 mRNA. These transcripts have identical 5' coding sequences (641 nucleotides); however, the 3' end of rTg-2 is comprised of coding and non-coding sequences not present in rTg-1. To determine if a single Tg gene encoded both mRNA species, a genomic clone was isolated which spanned the full-length rTg-2 cDNA sequence. The promoter sequence and restriction map were the same as for the previously characterized rTg-1 gene, indicating that rTg-1 and rTg-2 mRNAs are splicing variants derived from the same Tg gene. The unique 3' end of rTg-2 mRNA comprised a single exon which was intronic with respect to rTg-1 mRNA formation. The level of rTg-2 in cultured rat thyrocytes was more sensitive to thyrotropin (TSH) regulation than was rTg-1. rTg-2 mRNA was rapidly (and reversibly) depleted to nearly undetectable levels after TSH removal, unlike rTg-1. Conversely, TSH rapidly restored control levels of rTg-2 mRNA in such depleted cells. The data thus support a model of TSH-induced splicing and regulation of the two Tg mRNAs in the rat.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Graves PN,Davies TFdoi
10.1016/0303-7207(93)90126-5subject
Has Abstractpub_date
1993-06-01 00:00:00pages
213-8issue
2eissn
0303-7207issn
1872-8057journal_volume
93pub_type
杂志文章abstract：:A kallikrein-related protease was purified from rat ventral prostate cytosol by means of DEAE-Sepharose chromatography, followed by gel filtration on Sephadex G-100 and CM-cellulose chromatography. Antibodies raised in rabbits against the purified protease recognize two bands on immunoblots of prostatic cytosol: a 31,...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(89)90008-7
更新日期：1989-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2003-11-28 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2018-03-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：2006-03-27 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2009.02.028
更新日期：2009-08-13 00:00:00
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journal_title：Molecular and cellular endocrinology
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更新日期：1976-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-10-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2015-05-15 00:00:00
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更新日期：2018-07-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(86)90037-7
更新日期：1986-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-05-15 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/0303-7207(83)90057-6
更新日期：1983-11-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(00)00267-7
更新日期：2000-07-25 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(94)90242-9
更新日期：1994-05-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 临床试验,杂志文章
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更新日期：2007-01-29 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(94)90045-0
更新日期：1994-08-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(84)90124-2
更新日期：1984-08-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to address the role of low-density lipoprotein (LDL) on mouse preimplantation embryos. METHODS:The temporal expression of low-density lipoprotein receptor (LDLR) and side-chain cleavage cytochrome P450 (P450scc) mRNAs in mouse oocytes and preimplantation embryos up to the hatched bl...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：2003-04-28 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-11-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(86)90144-9
更新日期：1986-05-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 历史文章,杂志文章
doi：10.1016/j.mce.2003.10.008
更新日期：2004-03-31 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(93)90215-6
更新日期：1993-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2011-07-04 00:00:00
abstract：:Androgen-binding protein (ABP) is a testicular Sertoli cell secretory protein that acts as a carrier of androgen in the male reproductive tract. ABP has been characterized from a wide range of animal species, including man, rabbit and rat. However, it has been widely accepted that mice do not produce testicular ABP. W...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(89)90084-1
更新日期：1989-05-01 00:00:00
abstract：:The molecular basis of the organogenesis, homeostasis, and tumorigenesis of the adrenal cortex has been the subject of intense study for many decades. Specifically, characterization of tumor predisposition syndromes with adrenocortical manifestations and molecular profiling of sporadic adrenocortical tumors have led t...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2015.11.031
更新日期：2016-02-05 00:00:00
abstract：:Transcription elongation of many eukaryotic genes is regulated. Two negative transcription elongation factors, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) are known to stall collaboratively RNA polymerase II promoter proximally. We dis...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2010.01.020
更新日期：2010-05-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(02)00374-x
更新日期：2002-12-30 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2010.01.032
更新日期：2010-05-14 00:00:00
abstract：:The FOXO1 transcription factor is important for multiple aspects of reproductive function. We previously reported that FOXO1 functions as a repressor of gonadotropin hormone synthesis, but how FOXO1 is regulated in pituitary gonadotropes is unknown. The growth factors, insulin and insulin-like growth factor I (IGF1), ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2015.02.006
更新日期：2015-04-15 00:00:00
abstract：:Three chimeras of the rat GnRH receptor (rGnRHR) and an enhanced green fluorescent protein (GFP) were assessed to examine their suitability as probes of the receptor in transfected GH3 cells. Direct fusion of GFP to the N or C terminus of the rGnRHR abolished the receptor ligand binding affinity and the chimeric recep...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(98)00204-4
更新日期：1998-11-25 00:00:00
