Abstract：
:In human granulosa-lutein cells, high-density lipoproteins (HDL) can stimulate progesterone synthesis. The objective of the present study was to establish whether prostaglandins (PGs) participate in the steroidogenic response to HDL. Both HDL and apolipoprotein AI (ApoAI) stimulated concentration-dependent increases in PGE2, cAMP and progesterone accumulation. The minimum concentrations of HDL and ApoAI required to elevate PGE2 production were the same as those required to stimulate cAMP accumulation and progesterone synthesis. Concentrations of PGE2 were elevated within 10 min in cells exposed to HDL and rose progressively over 24 h, whereas cAMP and progesterone were only increased significantly after 24 h of treatment with HDL. Co-treatment with prostaglandin H synthase inhibitors (meclofenamic acid and indomethacin) abolished the cAMP and progesterone responses to both HDL and ApoAI. Hence, the ability of HDL to stimulate progesterone synthesis can be mimicked by ApoAI and appears to involve increased generation of one or more luteotrophic PGs, possibly acting via cAMP.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Chandras C,Ragoobir J,Barrett GE,Bruckdorfer KR,Graham A,Abayasekara DR,Michael AEdoi
10.1016/j.mce.2004.05.008keywords:
subject
Has Abstractpub_date
2004-07-30 00:00:00pages
1-8issue
1-2eissn
0303-7207issn
1872-8057pii
S030372070400214Xjournal_volume
222pub_type
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