Purification and N-terminal sequence analysis of Streptomyces chromofuscus phospholipase D.

Abstract:

:Partially purified commercial phospholipase D (PLD) was fractionated by dye-ligand affinity chromatography and nondenaturing polyacrylamide gel electrophoresis (PAGE). Active material migrated as three bands on SDS-PAGE. The two higher-abundance species were shown to have identical N-terminal sequences, while the third band was present in much smaller amounts and had a distinct sequence. Cloning Streptomyces chromofuscus PLD will allow the construction of stable transfectants of mast cell lines permitting regulated expression of PLD.

authors

Dinh TT,McClure GD,Kennerly DA

doi

10.1159/000236934

subject

Has Abstract

pub_date

1995-05-01 00:00:00

pages

69-71

issue

1-3

eissn

1018-2438

issn

1423-0097

journal_volume

107

pub_type

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