Abstract:
BACKGROUND:Basophils and mast cells are important initiator/effector cells capable of rapidly responding to IgE-mediated stimulation, but the precise mechanisms regulating their functions in vivo have not been fully identified. In this study, we assessed whether low levels of antigen can modulate activation of basophils and mast cells. METHODS:Human basophils and cultured mast cells were pretreated with low concentrations of anti-FcεRI α-chain mAb (CRA-1 mAb), and their cell functions were assessed. RESULTS:Basophils preincubated with CRA-1 mAb at as low as 1 ng/ml for 1 h showed significantly enhanced degranulation in response to various secretagogues such as MCP-1, FMLP, leukotriene B4 and Ca ionophore A23187. FMLP-induced leukotriene C4 production by basophils was also enhanced by CRA-1 mAb pretreatment. Degranulation was further enhanced when CRA-1 mAb-pretreated basophils were additionally treated with IL-3, IL-33 or leptin before stimulation with MCP-1. Priming by subthreshold CRA-1 mAb was a slow process, since 1 h of pretreatment was needed for maximal enhancement. Basophil priming also resulted from preincubation with subthreshold doses of an allergen, Der f 2. In parallel mAb experiments, CRA-1 mAb showed weak priming effects on human umbilical cord blood-derived cultured mast cells; a higher dose, 100 ng/ml, was necessary for this priming. CONCLUSION:These results indicate that subthreshold doses of CRA-1 mAb or allergens can prime basophils and induce exaggerated responses to various IgE-independent stimuli. This may be a potentially important mechanism that explains environmental allergen-induced exacerbation of IgE-mediated allergic diseases such as asthma.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Koketsu R,Yamaguchi M,Suzukawa M,Tanaka Y,Tashimo H,Arai H,Nagase H,Matsumoto K,Saito H,Ra C,Yamamoto K,Ohta Kdoi
10.1159/000350339subject
Has Abstractpub_date
2013-01-01 00:00:00pages
23-31eissn
1018-2438issn
1423-0097pii
000350339journal_volume
161 Suppl 2pub_type
杂志文章abstract:BACKGROUND:The so-called 'latex-fruit syndrome' is a well-documented phenomenon in cross-reactive allergies. By contrast, there is a lack of information about allergy to exotic fruits in patients with a predominant pollen sensitization. Since the ubiquitous protein profilin has been identified as an allergen in natural...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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abstract::Reversible airway obstruction and airway inflammation following allergen exposure represent crucial pathophysiological features of allergic asthma. In order to investigate these events, bronchial-specific challenge is commonly employed although it may be complicated and may not be free of possible risks. Therefore, we...
journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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doi:10.1159/000236681
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:International archives of allergy and immunology
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