Connective tissue growth factor does not affect transforming growth factor-beta 1-induced Smad3 phosphorylation and T lymphocyte proliferation inhibition.

Abstract:

:Transforming growth factor-beta 1 (TGF-beta(1)) is a key regulator of immune tolerance. TGF-beta(1) controls T lymphocyte activation and is involved in the immunosuppressive function and generation of regulatory T lymphocytes. Connective tissue growth factor (CTGF) has an essential role in the formation of connective tissue and blood vessels. CTGF expression is induced by TGF-beta(1) in several cell types and CTGF mediates several of the downstream actions of TGF-beta(1). Since little is known about the potential synergy between CTGF and TGF-beta(1) in T lymphocyte biology, the purpose of the present study was to determine whether CTGF can modulate TGF-beta(1)-mediated effects on human CD4+ T lymphocytes. Human recombinant CTGF was expressed in HEK293 cells. rCTGF was biologically active demonstrated by induction of proliferation in the endothelial cell line EA hy 926. rCTGF alone did not potentiate or diminish anti-CD3-induced CD4+ T lymphocyte proliferation and did not activate the Smad signaling pathway in CD4+ T lymphocytes. Furthermore, rCTGF did not attenuate TGF-beta(1)-mediated inhibition of CD4+ T lymphocyte proliferation and TGF-beta(1)-induced Smad signaling in CD4+ T lymphocytes. These results indicate that rCTGF had no detectable effects of its own on human CD4+ T lymphocytes and did not potentiate the effects of low amounts of TGF-beta(1) on human CD4+ T lymphocytes. Overall, these data support the hypothesis that CTGF does not act on CD4+ T lymphocytes.

authors

Kunzmann S,Seher A,Kramer BW,Schenk R,Schütze N,Jakob F,Sebald W,Speer CP

doi

10.1159/000137284

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

152-60

issue

2

eissn

1018-2438

issn

1423-0097

pii

000137284

journal_volume

147

pub_type

杂志文章
  • Immunologic effects of omalizumab in children with severe refractory atopic dermatitis: a randomized, placebo-controlled clinical trial.

    abstract:BACKGROUND:Severe refractory atopic dermatitis (AD) is a chronic, debilitating condition that is associated with elevated serum immunoglobulin E (IgE) levels. Thymic stromal lymphopoietin (TSLP), thymus and activation-regulated chemokine (TARC) and OX40 ligand (OX40L) are important immunologic factors involved in the p...

    journal_title:International archives of allergy and immunology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000350486

    authors: Iyengar SR,Hoyte EG,Loza A,Bonaccorso S,Chiang D,Umetsu DT,Nadeau KC

    更新日期:2013-01-01 00:00:00

  • 100 years of immunotherapy: the Monaco charter. under the high patronage of His Serene Highness Prince Albert II of Monaco.

    abstract::Aims of the Monaco Charter: (1) to present the current evidence on the efficacy and safety of allergen-specific immunotherapy (SIT) and to address the reasons for its underuse in clinical practice; (2) to develop strategies to increase the awareness about the benefits and the hazards of SIT in allergic patients, lay p...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000343883

    authors: Canonica GW,Baena-Cagnani CE,Compalati E,Bohle B,Bonini S,Bousquet J,Cox L,Fink-Wagner AH,González Díaz S,Jacobsen L,Passalacqua G,Pawankar R,Vieths S,Yusuf O,Zuberbier T

    更新日期:2013-01-01 00:00:00

  • Cytokine production by highly purified human CD8+ T cells.

    abstract::We have systematically investigated the capacity of highly purified human peripheral CD8+ T cells to produce interleukin (IL)-4 and interferon (IFN)-gamma when triggered by different stimuli. CD8+ T cells were isolated from peripheral blood by positive selection to > 99% purity and stimulated with one of three differe...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237030

    authors: Li Y,Richards D,Noble A,Kemeny DM

    更新日期:1995-05-01 00:00:00

  • Skin Care and Synbiotics for Prevention of Atopic Dermatitis or Food Allergy in Newborn Infants: A 2 × 2 Factorial, Randomized, Non-Treatment Controlled Trial.

    abstract:BACKGROUND:Atopic dermatitis (AD) and food allergy (FA) are common childhood diseases, which may either be interrelated or be the result of skin barrier disruption and gut mucosal dysbiosis. Although some evidence suggests the efficacy of emollients and synbiotics, there is no conclusive evidence on the use of these in...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000501636

    authors: Dissanayake E,Tani Y,Nagai K,Sahara M,Mitsuishi C,Togawa Y,Suzuki Y,Nakano T,Yamaide F,Ohno H,Shimojo N

    更新日期:2019-01-01 00:00:00

  • Inhibition of secretory phospholipase A2-induced cytokine production in human lung macrophages by budesonide.

    abstract:BACKGROUND:Secretory phospholipases A(2) (sPLA(2)) are an emerging class of mediators of inflammation. These enzymes are released in vivo in patients with systemic inflammatory diseases and allergic disorders. sPLA(2)s may activate inflammatory cells by both enzymatic and nonenzymatic mechanisms. The aim of this study ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000218117

    authors: Triggiani M,Granata F,Petraroli A,Loffredo S,Frattini A,Staiano RI,Monaco G,Marone G

    更新日期:2009-01-01 00:00:00

  • The Th1/Th2 paradigm and experimental murine leishmaniasis.

    abstract::In recent years, the Th1/Th2 concept has become of prime importance in the understanding of heterogeneous responses of the immune system and implications thereof for infectious and autoimmune diseases. Originally established on the basis of different cytokines produced by T cell clones, it is now known that the Th1/Th...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000023900

    authors: Lohoff M,Gessner A,Bogdan C,Röllinghoff M

    更新日期:1998-03-01 00:00:00

  • The activation of the JAK2/STAT5 pathway is commonly involved in signaling through the human IL-5 receptor.

    abstract::The JAK (Janus kinase) family of protein tyrosine kinases and the STATs (signal transducers and activators of transcription) have been shown to be activated in response to a number of cytokines and growth factors. In this study, we evaluated the activation of JAK/STAT pathway upon human interleukin-5 (hIL-5) stimulati...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237712

    authors: Ogata N,Kikuchi Y,Kouro T,Tomonaga M,Takatsu K

    更新日期:1997-10-01 00:00:00

  • Visualizing T cell migration in vivo.

    abstract::Ever since the realization that T lymphocytes are key players in the defense against pathogens and tumors, a major aim of immunologists has been to understand the relationship between the functional and migratory properties of antigen-specific T cells. The current paradigm proposes that T cells follow organ-specific t...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000074896

    authors: Iparraguirre A,Weninger W

    更新日期:2003-12-01 00:00:00

  • The regulatory system in europe with special emphasis on allergen products.

    abstract::For each medicinal product quality, safety and efficacy have to be proven to obtain a marketing authorisation. The national competent health authorities and the European Medicines Agency (EMEA) with support of the Heads of Medicines Agencies (HMA) work together to grant marketing authorisations for medicinal products....

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000146074

    authors: Lorenz AR,Luttkopf D,Seitz R,Vieths S

    更新日期:2008-01-01 00:00:00

  • Isolation and preparation of antisera to ovine IgE.

    abstract::Elevated levels of ovine reaginic antibody were induced by immunization with Ascaris suum antigens. The PCA activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees C, suggesting that it was immunoglobulin E. Attempts to isolate IgE from this hyperimmune serum by ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236479

    authors: Yilmaz H,Roe JM,Morgan KL

    更新日期:1993-01-01 00:00:00

  • Defective 'gut processing' of gliadin in mice with graft-versus-host enteropathy.

    abstract::The gut mucosa plays an important role in the induction of oral tolerance. Extending previous observations, we have here shown that serum containing gut-absorbed gliadin induces suppression of the specific systemic immune response in recipient mice parenterally immunized with gliadin. Graft-versus-host reaction (GvHR)...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237230

    authors: Troncone R,Caputo N,Zibella A,Russo R,Rossi M,Gianfrani C,Stern M,Wieser H,Auricchio S

    更新日期:1996-01-01 00:00:00

  • Glutamine Prevents Late-Phase Anaphylaxis via MAPK Phosphatase 1-Dependent Cytosolic Phospholipase A2 Deactivation.

    abstract:BACKGROUND:Cytosolic phospholipase A2 (cPLA2) plays a key role in the development of late-phase anaphylaxis. L-Glutamine (Gln), a nonessential amino acid, has anti-inflammatory activity via inhibiting cPLA2. METHODS:We used a penicillin-induced murine model of anaphylaxis, and late-phase anaphylaxis was quantified by ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000452103

    authors: Kim HK,Song CH,Bae YS,Im SY,Lee HK

    更新日期:2016-01-01 00:00:00

  • Concordance of lateral flow and skin prick tests in the assessment of allergen sensitisation in the epidemiological field in children.

    abstract:BACKGROUND:A new lateral flow test (LFT) for office use provides information about sensitisation to common allergens. Concordance between LFT and skin-prick test (SPT) has not been assessed in epidemiological studies. The aim of this study is to determine LFT-SPT concordance in this field. METHODS:Plasma samples (n = ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000288291

    authors: Sanchez-Bahillo M,Garcia-Marcos L,Martinez-Torres AE,Perez-Fernandez V,Sanchez-Solis M

    更新日期:2010-01-01 00:00:00

  • Influence of Omalizumab on Allergen-Specific IgE in Patients with Adult Asthma.

    abstract:BACKGROUND:Omalizumab, an anti-immunoglobulin E (IgE) monoclonal antibody, inhibits the binding of circulating IgE to mast cells and basophils, resulting in fewer episodes of airway inflammation, asthma symptoms and exacerbations in patients with severe allergic asthma. Treatment of patients with asthma using omalizuma...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000442668

    authors: Mizuma H,Tanaka A,Uchida Y,Fujiwara A,Manabe R,Furukawa H,Kuwahara N,Fukuda Y,Kimura T,Jinno M,Ohta S,Yamamoto M,Matsukura S,Adachi M,Sagara H

    更新日期:2015-01-01 00:00:00

  • Platelet-activating-factor-induced augmentation of production of eosinophil-lineage cells in hematopoietic precursor cells obtained from human umbilical cord blood.

    abstract::Platelet-activating factor (PAF)-induced augmentation of spontaneous differentiation into eosinophil-lineage cells from hematopoietic precursor cells was found among human umbilical cord blood mononuclear cells (CBMCs). This ability of PAF to augment eosinophil production was significantly (p < 0.01) inhibited by the ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236586

    authors: Saito H,Koshio T,Yanagihara Y,Akiyama K,Shida T

    更新日期:1993-01-01 00:00:00

  • Morphology and three-dimensional reconstruction of the digestive system of Dermatophagoides farinae.

    abstract:BACKGROUND:The digestive system of Dermatophagoides farinae remains poorly documented. METHODS:In this study, a three-dimensional (3-D) model of the alimentary canal of D. farinae was built for the first time based on hematoxylin-eosin staining, 76 cross-sections of the adult mite and 3-D reconstruction technology. R...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000115890

    authors: Zhang YY,Sun X,Liu ZG

    更新日期:2008-01-01 00:00:00

  • Enhancement of drug-specific lymphocyte proliferation using CD25(hi)-depleted CD3(+) effector cells.

    abstract:BACKGROUND:The lymphocyte transformation test (LTT) is used for in vitro diagnosis of drug hypersensitivity reactions. While its specificity is over 90%, sensitivity is limited and depends on the type of reaction, drug and possibly time interval between the event and analysis. Removal of regulatory T cells (Treg/CD25(h...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000358491

    authors: Srinoulprasert Y,Pichler WJ

    更新日期:2014-01-01 00:00:00

  • Mast cell heterogeneity: evidence that mast cells isolated from various connective tissue locations in the rat display markedly graded phenotypes.

    abstract::The present study has extended histochemical and functional investigations into rat mast cell heterogeneity using isolated mast cells from four connective tissue locations; the peritoneum, mesentery, lung and skin. On histological examination, mast cells from these locations displayed a range of phenotypes following f...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236161

    authors: Tainsh KR,Pearce FL

    更新日期:1992-01-01 00:00:00

  • Antigen-induced reduction in mast cell and basophil functional responses due to reduced Syk protein levels.

    abstract:BACKGROUND:The high-affinity IgE receptor, FcepsilonRI, is unresponsive on mast cells and basophils from people in several populations through an unknown mechanism. Similarly, FcepsilonRI-positive basophils from 'nonreleasers' are IgE-unresponsive and are deficient in the tyrosine kinase Syk. OBJECTIVE:To test the hyp...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000087355

    authors: Kepley CL

    更新日期:2005-09-01 00:00:00

  • Mast cell hyperplasia: role of cytokines.

    abstract::Mast cell hyperplasia is found in different pathologies such as chronic inflammatory processes, fibrotic disorders, wound healing or neoplastic tissue transformation. The functional significance of the accumulation of mast cells in these processes is largely unknown. It is now established that bone marrow-derived mast...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000048181

    authors: Bischoff SC,Sellge G

    更新日期:2002-02-01 00:00:00

  • Sex Differences in Adults with Insect Venom Allergy regarding Psychological Distress, Attitudes toward the Emergency Medication, and Factual Knowledge about the Disease.

    abstract:BACKGROUND:Hymenoptera venom allergy (HVA) has a prevalence of 3% in adults. Although patients have no ongoing symptoms, they often suffer from an impairment of their psychological well-being and quality of life. OBJECTIVE:The aim of this study was to analyze sex-specific differences regarding the psychological burden...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000507625

    authors: Schoeben LS,Bubak C,Schaarschmidt ML,Schmieder A

    更新日期:2020-01-01 00:00:00

  • Isolation and characterization of two cDNA clones coding for isoforms of the Parietaria judaica major allergen Par j 1.0101.

    abstract::Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. Sequence analysis showed open reading frames of 176 and 138 amino acids. Both ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237479

    authors: Duro G,Colombo P,Assunta Costa M,Izzo V,Porcasi R,Di Fiore R,Locorotondo G,Cocchiara R,Geraci D

    更新日期:1997-04-01 00:00:00

  • Peptide specificity, HLA class II restriction, and T-cell subsets of the T-cell clones specific to either Cry j 1 or Cry j 2, the major allergens of Japanese cedar (Cryptomeria japonica) pollen.

    abstract:BACKGROUND:Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are not well known. METHODS:CD4+ T (Th)-cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were g...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000024194

    authors: Sone T,Morikubo K,Shimizu K,Komiyama N,Tsunoo H,Kino K

    更新日期:1999-07-01 00:00:00

  • Mechanisms of action of antiphospholipid antibodies in the antiphospholipid syndrome.

    abstract::Antiphospholipid antibody syndrome (APS) is characterized by autoantibodies against negatively charged phospholipids in the serum, and clinically by multiple thromboses, thrombocytopenia, and recurrent fetal loss. The mechanism(s) by which the antibodies cause the clinical picture are not clear. We review the possible...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000236883

    authors: Aron AL,Gharavi AE,Shoenfeld Y

    更新日期:1995-01-01 00:00:00

  • Protein tyrosine phosphorylation: A possible common signaling pathway in human Th1 and Th2 cell clones.

    abstract:BACKGROUND:It has been reported that protein tyrosine phosphorylation is essential for cytokine production in mouse Th1 cells but not in Th2 cells. However, little is known about the difference of signal transduction between human antigen-specific Th1 and Th2 cell clones. METHODS:Purified protein derivative-specific T...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000024028

    authors: Minoguchi K,Yamashita N,Oda N,Takeno M,Kaneoka H,Sakane T

    更新日期:1999-01-01 00:00:00

  • Mite-Induced Asthma and IgE Levels to Shrimp, Mite, Tropomyosin, Arginine Kinase, and Der p 10 Are the Most Relevant Risk Factors for Challenge-Proven Shrimp Allergy.

    abstract:BACKGROUND:Shrimp sensitization is common in the general population, but the presence of symptoms is only moderately related to sensitization. A point still at issue is which in vivo and/or in vitro tests (food challenge, component-resolved diagnosis, house dust mite [HDM] sensitization) can help in distinguishing shri...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000481985

    authors: Farioli L,Losappio LM,Giuffrida MG,Pravettoni V,Micarelli G,Nichelatti M,Scibilia J,Mirone C,Cavallarin L,Lamberti C,Balossi LG,Pastorello EA

    更新日期:2017-01-01 00:00:00

  • Phenotypic and Functional Analysis of T Follicular Cells in Common Variable Immunodeficiency.

    abstract:INTRODUCTION:One of the most frequent abnormalities of B cells in common variable immunodeficiency (CVID) is reduced number of class-switched memory B cells, suggesting an impaired germinal center response. Therefore, due to its pivotal role in regulating the development of humoral immunity, the objective of this study...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000507995

    authors: Kasahara TM,Bento CAM,Gupta S

    更新日期:2020-01-01 00:00:00

  • Characterization of dog allergens Can f 1 and Can f 2. 1. Preparation of their recombinant proteins and antibodies.

    abstract:BACKGROUND:Recombinant dog allergens, rCan f 1 and rCan f 2, and their antibodies are good tools for the characterization of dog allergens in order to develop modern therapeutic and preventive methods for dog allergy. METHODS:In this study, cDNA was synthesized from the mRNA of dog salivary glands and cloned into the ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000097498

    authors: Kamata Y,Miyanomae A,Nakayama E,Miyanomae T,Tajima T,Hoshi H

    更新日期:2007-01-01 00:00:00

  • Isoforms of atopic allergens with reduced allergenicity but conserved T cell antigenicity: possible use for specific immunotherapy.

    abstract:BACKGROUND:We analyzed the T cell activation potency and the IgE-binding properties (allergenicity) of nine isoforms of Bet v 1, the major allergen of birch pollen. METHODS:The capacity of recombinant Bet v 1 isoforms to bind serum IgE from allergic patients was evaluated by immunoblot experiments and skin prick tests...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237524

    authors: Ferreira F,Hirthenlehner K,Briza P,Breiteneder H,Scheiner O,Kraft D,Breitenbach M,Ebner C

    更新日期:1997-05-01 00:00:00

  • Comparison of histamine release from human blood monocytes, lymphocytes, adenoidal and skin mast cells.

    abstract::Monocytes and lymphocytes from human blood contain 0.043 +/- 0.007 and 0.053 +/- 0.014 pg histamine/cell, respectively, which can be released by a number of stimulants (A 23187, C5a, substance P, specific allergen). The release process takes 60-120 min to reach its end point, in contrast to tissue mast cells which com...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236974

    authors: Schmutzler W,Bolsmann K,Zwadlo-Klarwasser G

    更新日期:1995-05-01 00:00:00