Abstract：
:The thrombocyte is the avian equivalent of the mammalian blood platelet and is involved in hemostasis through a fibrinogen-mediated process. Although fibrinogen has been implicated as a molecular bridge between activated cells during aggregation, the location of this molecule and its receptor on thrombocytes has not been characterized. Pigeon fibrinogen, isolated from plasma by precipitation with PEG-1000 and purified over Sepharose 4B, was used to study receptor-ligand interaction. Separation of pigeon fibrinogen on SDS-PAGE resulted in three peptides of molecular mass 62, 55, and 47 kDa, which were comparable to those of human fibrinogen. The role of fibrinogen and its receptor in thrombocyte function was established by turbidimetric aggregation using thrombin as an agonist under conditions requiring Ca2+ and fibrinogen. Maximum response occurred using 3 mM Ca2+ and 100 micrograms/ml fibrinogen. Fibrinogen-dependent aggregation was inhibited by an anti-GPIIb antibody, verifying a role for fibrinogen receptors in thrombocyte function. Fibrinogen-gold conjugates were used to describe receptor and ligand localization on aggregated cells. Computer reconstruction was used to verify relocalization of fibrinogen receptors following activation. Fibrinogen distribution changed from a dispersed state in preactivated cells to focal localization at points of cell contact and along pseudopods following activation. This selective positioning of fibrinogen suggests that a functional relocalization of the receptor occurs upon thrombocyte activation, and this relocation facilitates the role of fibrinogen as a molecular bridge. These studies establish similarities between the avian and the human systems and document the conserved nature of the hemostatic process.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
O'Toole ET,Hantgan RR,Lewis JCdoi
10.1006/exmp.1994.1035subject
Has Abstractpub_date
1994-12-01 00:00:00pages
175-90issue
3eissn
0014-4800issn
1096-0945pii
S0014-4800(84)71035-5journal_volume
61pub_type
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2240
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更新日期：1985-12-01 00:00:00
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更新日期：2009-10-01 00:00:00
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更新日期：2015-12-01 00:00:00
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doi：10.1016/j.yexmp.2012.04.017
更新日期：2012-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.yexmp.2013.08.005
更新日期：2013-12-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：1992-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90003-0
更新日期：1984-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2001.2397
更新日期：2001-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.08.002
更新日期：2005-12-01 00:00:00
abstract：:Several studies recognize cancer-stromal fibroblasts' role in cancer-cell invasion and metastasis. Through paracrine signaling molecules, TGF-beta and IL-1beta, cancer cells activate stromal fibroblasts and induce the expression of fibroblast activation protein (FAP). FAP, in turn, affects the proliferation, invasion ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.09.001
更新日期：2009-12-01 00:00:00
abstract：:Paraoxonase gene polymorphisms (PON1-55 and PON1-192) were determined in four Mexican populations (Mestizos, Nahuas, Teenek and Mayos) belonging to different ethnic groups. The role of these polymorphisms in the lipid profile in the Mestizo group was also analyzed. PON1 polymorphisms were determined by polymerase chai...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.05.006
更新日期：2006-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2003-04-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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doi：10.1016/j.yexmp.2012.04.023
更新日期：2012-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2002.2423
更新日期：2002-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-06-01 00:00:00
abstract：:Studies of spontaneous mutations in mice have provided valuable disease models and important insights into the mechanisms of human disease. Ruffled (rul) is a new autosomal recessive mutation causing abnormal hair coat in mice. The rul allele arose spontaneously in the RB156Bnr/EiJ inbred mouse strain. In addition to ...
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更新日期：2015-04-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(90)90025-9
更新日期：1990-08-01 00:00:00
abstract：:Primary hepatic peripheral T-cell lymphoma (PTCL) is exceedingly rare. We encountered such a case in a 58-year-old Hispanic female with a history of chronic sinusitis and hypothyroidism who presented with 4weeks of fever and weight loss. Laboratory studies showed altered liver function and mild pancytopenia. Hepatitis...
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更新日期：2016-02-01 00:00:00
abstract：:In this study we investigated the effects of a mild toxic challenge at selected points in time on the nonspecific cellular events that occurred in acutely damaged pulmonary alveoli. Swiss-Webster mice were treated with butylated hydroxytoluene (BHT, 400 mg/kg ip) and sacrificed at Days 1, 3, and 5 thereafter; either 2...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
abstract：:Tumor associated macrophages (TAMs) play an important role in the growth, progression, and metastasis of tumors. The distribution of TAMs in Kazakh esophageal squamous cell carcinoma (ESCC) is not determined. We aimed to investigate the role of TAMs in the occurrence and progression of Kazakh ESCC. CD163 was used as t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
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pub_type： 杂志文章
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更新日期：2015-05-07 00:00:00
abstract：:Cell death caused by the accumulation of extracellular adenosine is believed to contribute to the profound loss of T lymphocytes in patients with severe combined immunodeficiency disease due to adenosine deaminase deficiency. Although adenosine is known to trigger apoptosis in thymocytes and peripheral T cells, the mo...
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更新日期：2005-12-01 00:00:00
