Abstract：
:To assess elastin biosynthesis in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model. The time points of the study were 1, 3, and 7 days and 2, 4, and 8 weeks after coarctation. Additional animals were subjected to hypercholesterolemia for analysis of tropoelastin expression in the intimal lesion. mRNA for tropoelastin was quantitated by Northern blot analysis and its distribution was revealed by in situ hybridization. The 65-kDa tropoelastin was analyzed by Western blotting and immunohistochemistry. Tropoelastin mRNA proximal to the coarctation was increased at 2 weeks and returned to baseline by 8 weeks (P < 0.05 versus control). Changes in 65-kDa tropoelastin corresponded to those of mRNA. Tropoelastin gene was expressed mainly in the intima and in the outer media at the proximal region to the stenoses, which was particularly remarkable in the intimal lesion. The results indicate that tropoelastin gene expression was enhanced in the early remodeling response to elevated blood pressure. The distribution of newly synthesized tropoelastin in the outer media suggests a reenforcement role of tropoelastin, which preserves mechanical resiliency in response to changes in tensile stress.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Xu C,Zarins CK,Glagov Sdoi
10.1006/exmp.2002.2423keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
115-23issue
2eissn
0014-4800issn
1096-0945pii
S0014480002924238journal_volume
72pub_type
杂志文章abstract：:We tested the hypothesis that structural changes in the genome parallel age- and organ-specific phenotypes in conjunction with the differential transposition activities of retroelements. The genomes of the liver from C57BL/6J mice were larger than other organs, coinciding with an increase in genomic copies of certain ...
journal_title：Experimental and molecular pathology
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pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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pub_type： 杂志文章,评审
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2013.12.004
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：1994-12-01 00:00:00
