Abstract：
:Ischemia has elicited a great deal of interest among the scientific community due to its role in life-threatening pathologies such as cancer, stroke, acute renal failure, and myocardial infarction. Oxygen deprivation (hypoxia) associated with ischemia has recently become a subject of intense scrutiny. New investigators may find it challenging to induce hypoxic injury in vitro. Researchers may not always be aware of the experimental barriers that contribute to this phenomenon. Furthermore, ischemia is associated with other major insults, such as excess carbon dioxide (hypercapnia), nutrient deprivation, and accumulation of cellular wastes. Ideally, these conditions should also be incorporated into in vitro models. Therefore, the motivation behind this review is to: i. delineate major in vivo ischemic insults; ii. identify and explain critical in vitro parameters that need to be considered when simulating ischemic pathologies; iii. provide recommendations to improve experiments; and as a result, iv. enhance the validity of in vitro results for understanding clinical ischemic pathologies. Undoubtedly, it is not possible to completely replicate the in vivo environment in an ex vivo model system. In fact, the primary goal of many in vitro studies is to elucidate the role of specific stimuli during in vivo pathological events. This review will present methodologies that may be implemented to improve the applicability of in vitro models for understanding the complex pathological mechanisms of ischemia. Finally, although these topics will be discussed within the context of renal ischemia, many are pertinent for cellular models of other organ systems and pathologies.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Russ AL,Haberstroh KM,Rundell AEdoi
10.1016/j.yexmp.2007.03.002subject
Has Abstractpub_date
2007-10-01 00:00:00pages
143-59issue
2eissn
0014-4800issn
1096-0945pii
S0014-4800(07)00039-1journal_volume
83pub_type
杂志文章,评审abstract：:Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2002.2470
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVES:Ethanol has been considered as a lifestyle risk factor for cancer in humans. While some studies have indicated that alcohol intake has a preventive effect for renal cell cancer, others have not. The metabolism of alcohol in cancer cells may be in many ways different than in healthy tissue and its disturbance...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.03.012
更新日期：2015-06-01 00:00:00
abstract：OBJECT:Mutations of the gene encoding isocitrate dehydrogenase (IDH) have been shown in a significant proportion of diffuse gliomas. These mutations are specific to gliomas and their utility for diagnosis and prognostication of these tumors is being proclaimed. The present study was conducted with the aim of assessing ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2011.04.017
更新日期：2011-08-01 00:00:00
abstract：:The Chediak-Higashi syndrome (CHS) is an inherited disorder of humans and of several animal species, characterized by partial albinism, pseudohemophilia, increased susceptibility to disease, and large inclusions in all granule-forming cells. In this study, various parameters of adenine nucleotide biochemistry were exa...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1993.1004
更新日期：1993-02-01 00:00:00
abstract：:Tumor associated macrophages (TAMs) play an important role in the growth, progression, and metastasis of tumors. The distribution of TAMs in Kazakh esophageal squamous cell carcinoma (ESCC) is not determined. We aimed to investigate the role of TAMs in the occurrence and progression of Kazakh ESCC. CD163 was used as t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.12.001
更新日期：2017-02-01 00:00:00
abstract：:Sprague Dawley male rats (200-220 g) were subjected to a long-term treatment with low doses of catecholamines in an attempt to assess the effect of the adrenergic stimulation on ventricular weights, hemodynamics, and hydroxyproline content in the myocardium. Data are presented indicating that both exogenous catecholam...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90028-5
更新日期：1984-12-01 00:00:00
abstract：:We investigated the protein carbonylation of red blood cell (RBC) membrane in type 2 diabetic patients and the potential implication of carbonyl/oxidative stress in reflecting disease severity. Sixty-four diabetic patients with or without retinopathy of variable clinical severity (Groups DR and DM, respectively) and 2...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.04.001
更新日期：2009-08-01 00:00:00
abstract：PURPOSE:Tumor surgery is aimed at complete resection of the lesion while ensuring a sufficient tumor-specific safety distance. Nevertheless, in many cases the most peripheral part - the invasion front - remains in situ. Tumor cells at the tumor margin have been reported to lose their epithelial properties and acquire f...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2017.02.013
更新日期：2017-04-01 00:00:00
abstract：:The purpose of this study was to better establish the morphologic basis of the early mucosal lesions of experimental inflammatory bowel disease because understanding the development of these lesions has important pathogenetic implications. Sprague-Dawley rats were given 1.5% hydrolyzed lambda-carrageenan orally for 30...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1994.1011
更新日期：1994-04-01 00:00:00
abstract：:We cloned and partially characterized a human endonuclease (Xib) which shows sequence homologies to pancreatic DNase I but an enzymatic activity closer to DNase II. We report on the structural differences found between Xib and other recently cloned human DNases. Fluores cence microscopy analysis of transiently transfe...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2254
更新日期：1999-06-01 00:00:00
abstract：:The amelioration of fetal alcohol effects on the postnatal development of the Purkinje cell by exogenous L-thyroxine was investigated in the neonatal rat. Time-pregnant rats were divided into three groups. Group A (n = 6) received 35% liquid ethanol diet; Group B (n = 6) was fed a liquid diet in which maltose dextrins...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2283
更新日期：1999-12-01 00:00:00
abstract：:Myelofibrosis is characterized by reticulin and/or collagen fibrosis in the bone marrow stroma resulting in secondary cytopenia. In addition to clonal hematologic neoplasms, myelofibrosis may also develop in association with other clinical conditions, including hematological disorders, solid malignancies, Down syndrom...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.07.004
更新日期：2014-10-01 00:00:00
abstract：:Changes in lung structure and collagen metabolism were studied at 1, 2, 3, 4, 6, and 8 weeks in a model of pulmonary fibrosis induced in rats with paraquat plus hyperoxia. Morphologic examination of the lungs revealed that the earliest lesions consisted of severe and irreversible endothelial and alveolar epithelial ce...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(89)90027-0
更新日期：1989-04-01 00:00:00
abstract：:The most common recurrent translocation in clear cell sarcoma t(12;22)(q13;q12) results in an EWSR1/ATF1 chimeric gene. We present a molecular analysis of tumor overgrowing right proximal tibia with bone destruction metastatic to two groin lymph nodes. Fluorescent in situ hybridization analysis performed on paraffin-e...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.12.005
更新日期：2011-04-01 00:00:00
abstract：:RNA binding motif protein 3 (RBM3) has been shown to be upregulated in several types of human tumors. Using tissue microarrays and immunohistochemistry, we showed here that both nuclear and cytoplasmic RBM3 expression levels were higher in hepatocellular carcinoma (HCC) tissues than in adjacent non-tumorous tissues. H...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104546
更新日期：2020-12-01 00:00:00
abstract：:The effects of chronic treatment with isoproterenol, reserpine, prenalterol, and terbutaline on rat parotid gland were investigated by electron microscopy and X-ray microanalysis. Chronic isoproterenol treatment induced lower potassium and calcium concentrations in the acinar cells. The cells were enlarged and contain...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90024-8
更新日期：1984-12-01 00:00:00
abstract：:Previous studies demonstrated that the anti-phosphorylcholine (PC) antibodies produced by aged BALB/c (18-22 months old) mice are structurally different and less protective against infection with Streptococcus pneumoniae than those produced by young (3-4 months old) syngeneic mice. However, PC antibody from aged anima...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1011
更新日期：1995-04-01 00:00:00
abstract：:We have modified methods of growing human gallbladder epithelial cells in monolayer and organotypic culture. These cells were grown in the presence of fetal bovine serum and with coculture of feeder layers of human gallbladder fibroblasts. Human gallbladders were obtained from cholecystectomy specimens, and the cells ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1026
更新日期：1995-08-01 00:00:00
abstract：:The components of the insulin-like growth factor (IGF) system and molecules with which they interact are associated with the neoplastic transformation of cells in colorectal cancer. The IGF-binding protein-2 (IGFBP-2) plays a significant role in mitotic stimulation of the cancer cells and its concentration is signific...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2013.11.006
更新日期：2014-02-01 00:00:00
abstract：:Sabaeksan has been used for prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of Sabaeksan, we examined the effect of Sabaeksan on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced pro-inflammatory cytokines secretion in human m...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.01.001
更新日期：2005-06-01 00:00:00
abstract：:Cancer develops when cells no longer follow their normal pattern of controlled growth. In the absence or disregard of such regulation, resulting from changes in their genetic makeup, these errant cells acquire a growth advantage, expanding into precancerous clones. Over the past decade many studies have revealed the r...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/s0014-4800(03)00010-8
更新日期：2003-04-01 00:00:00
abstract：:Megalencephaly-polymicrogyria-polydactyly-hydrocephalus (MPPH) syndrome is a developmental brain disorder characterized by an enlarged brain size with bilateral perisylvian polymicrogyria and a variable degree of ventriculomegaly. MPPH syndrome is associated with oromotor dysfunction, epilepsy, intellectual disability...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104471
更新日期：2020-08-01 00:00:00
abstract：:We previously reported the expression profiles of 9 cytochrome P450 isozymes (CYPs) proteins and those of 40 CYPs genes in pregnant rat's liver, placenta and fetal liver after treatment with pregnenolone-16alpha-carbonitrile (PCN) or phenobarbital (PB). This study was carried out focusing on the gene expression profil...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.08.002
更新日期：2005-12-01 00:00:00
abstract：:Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computat...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.104360
更新日期：2020-02-01 00:00:00
abstract：:5-Azacytidine (AZC) was studied in a lung cancer model in outbred and syngeneic (F1D) hamsters wherein benzol[a]pyrene (BP) from sustained release implants (SRI) induces preneoplastic mucosal changes which progress to bronchogenic cancer. In pilot studies to evaluate AZC toxicity, a dose schedule of 5 mg/kg biweekly w...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(90)90022-6
更新日期：1990-08-01 00:00:00
abstract：:Severe sepsis remains an important cause of death, particularly among trauma and burn patients. The severity of the systemic inflammatory response after trauma or burns and susceptibility to sepsis vary among individuals. One possible mechanism is through differential effects on glucocorticoid receptor (GR) expression...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2008.04.003
更新日期：2008-06-01 00:00:00
abstract：BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) could differentiate into cardiomyocytes. In this study, we wished to determine whether we could improve ventricular function in a dilated cardiomyopathy (DCM) model in the rabbit by injecting stem cells directly into the ventricular wall. METHODS AND RESULTS:Japane...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.12.002
更新日期：2010-04-01 00:00:00
abstract：:Periostin is a modular glycoprotein frequently observed to be a major constituent of the extracellular milieu of mass-forming intrahepatic cholangiocarcinoma and other desmoplastic malignant tumors. In intrahepatic cholangiocarcinoma, as well as in desmoplastic pancreatic ductal adenocarcinoma, periostin is overexpres...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2014.10.007
更新日期：2014-12-01 00:00:00
abstract：:Plasma fibronectin modulates reticuloendothelial (RE) phagocytosis of cellular and tissue debris, fibrin microaggregates, and gelatin-coated particulates. An antigenically related, but more insoluble form of fibronectin is found in various tissues and suspected to play a role in vascular permeability, cell adhesion, a...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90010-8
更新日期：1984-08-01 00:00:00
abstract：:Huntingtin-interacting protein 1-related (HIP1R) is an endocytic protein involved in receptor trafficking, including regulating cell surface expression of receptor tyrosine kinases. We have previously shown that low HIP1R protein expression was associated with poorer survival in diffuse large B-cell lymphoma (DLBCL) p...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.yexmp.2015.08.019
更新日期：2015-12-01 00:00:00
