Abstract:
:Plasma fibronectin modulates reticuloendothelial (RE) phagocytosis of cellular and tissue debris, fibrin microaggregates, and gelatin-coated particulates. An antigenically related, but more insoluble form of fibronectin is found in various tissues and suspected to play a role in vascular permeability, cell adhesion, and wound healing. The current study developed a fluorescent immunoassay which could be utilized for the quantification of tissue fibronectin following its extraction from tissues. Additionally, the changes in tissue fibronectin induced by the intravenous injection of gelatin-coated colloids in rats (300-350 g), which acutely depletes the plasma fibronectin level, were also investigated. Injection of gelatinized RE test lipid emulsion (50 mg/100 g) depleted the plasma fibronectin at 2 hr (80-85% depletion) followed by rebound restoration within 24 hr as quantified by either electroimmunoassay or fluorescent immunoassay. RES system clearance of the test particles from the blood resulted in an acute elevation in fibronectin extractable from the liver with a normalization by 48 hr. In contrast, assay of tissue fibronectin following a single extraction revealed a decrease in lung extractable fibronectin within 2 hr following RE blockade which persisted for 24-48 hr. Extractable fibronectin in spleen and renal tissue was unaltered by RE blockade. This microfluorescent immunoassay may provide a sensitive method to quantify fibronectin in small aliquots of tissue. Increased hepatic fibronectin most likely reflects interiorization of plasma fibronectin during Kupffer cell clearance of the test particles. Decreased lung extractable fibronectin may alter lung vascular sensitivity to a subsequent septic and/or intravascular coagulation stress. Thus, similar to the labile nature of plasma fibronectin, the concentration of fibronectin in various tissues, can undergo dynamic alterations.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Saba TM,Cho E,Blumenstock FAdoi
10.1016/0014-4800(84)90010-8subject
Has Abstractpub_date
1984-08-01 00:00:00pages
81-95issue
1eissn
0014-4800issn
1096-0945pii
0014-4800(84)90010-8journal_volume
41pub_type
杂志文章abstract::miR-126 has been implicated in the processes of inflammation and angiogenesis. Through these processes, miR-126 is implicated in cancer biology, but its role there has not been well reviewed. The aim of this review is to examine the molecular mechanisms and clinicopathological significance of miR-126 in human cancers....
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2013.12.004
更新日期:2014-02-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2008.07.001
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journal_title:Experimental and molecular pathology
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.05.008
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2011.07.003
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abstract::Deletion in the 22.9 -Mb chromosomal (chr.) 8p21.3-23 region has been shown to be necessary for the development of breast carcinoma (CaBr). In this study, we have attempted to detect the minimal deleted region(s) in the chr.8p21.3-23 region in 62 primary breast lesions having 56 CaBr tumors and six other breast lesion...
journal_title:Experimental and molecular pathology
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doi:10.1016/j.yexmp.2004.01.002
更新日期:2004-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2012.04.013
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.05.004
更新日期:2007-12-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1999.2275
更新日期:1999-10-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.01.004
更新日期:2007-04-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2016.03.010
更新日期:2016-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(92)90037-c
更新日期:1992-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章,多中心研究
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更新日期:2015-12-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(91)90046-z
更新日期:1991-10-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(89)90016-6
更新日期:1989-10-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2003.10.007
更新日期:2004-04-01 00:00:00
abstract::Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression was reported in various kinds of tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in pancrea...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.01.010
更新日期:2010-06-01 00:00:00
abstract::The cytotoxic and DNA-damaging effects of the pancreatic carcinogens azaserine, streptozotocin, and N-nitrosobis(2-oxyopropyl)amine (BOP) on propagable cultured normal rat pancreatic epithelial cells have been studied. All three chemicals in micromolar concentrations produced cytotoxicity to these cells. The concentra...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(90)90044-e
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2013.07.009
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2015.06.002
更新日期:2015-08-01 00:00:00
abstract::The keratinocyte growth factor (KGF) regulates cell growth and behavior in an autocrine or paracrine manner. In colorectal cancer tissues, KGF is expressed in tumor cells and adjacent stromal fibroblasts. We have constructed a KGF-gene-transfected cell line (HCT15-KGF) from a colorectal cancer cell line, HCT-15, that ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.07.001
更新日期:2007-12-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(91)90014-o
更新日期:1991-08-01 00:00:00
abstract::Reproductive performances of female hamsters were investigated during Ancylostoma ceylanicum (hookworm) infection. Animals having the highest levels of infection (34.96 +/- 1.11 worms) showed degenerative changes in the reproductive system. Ovaries of infected animals contained a few primary or secondary follicles. On...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(92)90043-b
更新日期:1992-08-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2014.09.010
更新日期:2014-12-01 00:00:00
abstract::We have investigated the incidence of the C677T and A1298C methylene tetrahydrofolate reductase (MTHFR) gene single nucleotide polymorphisms (SNPs) in the South Indian Tamil Nadu population with a total number of 72 individuals. The MTHFR genotyping was performed using the polymerase chain reaction followed by restric...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.04.006
更新日期:2004-10-01 00:00:00
abstract::To investigate the role of N-acetylglucosaminyltransferases V (GnT-V) in the malignancy of human hepatocellular carcinoma (HCC), the GnT-V stably suppressed cell line HepG2 GnT-V/1564 was constructed from HepG2. The proliferation, migration, invasion, metastasis of HepG2 GnT-V/1564 was investigated both in vitro and i...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2012.01.011
更新日期:2012-08-01 00:00:00
abstract:PURPOSE:Tumor surgery is aimed at complete resection of the lesion while ensuring a sufficient tumor-specific safety distance. Nevertheless, in many cases the most peripheral part - the invasion front - remains in situ. Tumor cells at the tumor margin have been reported to lose their epithelial properties and acquire f...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2017.02.013
更新日期:2017-04-01 00:00:00
abstract::We have produced experimental diffuse interstitial pulmonary fibrosis in rats with a combination of low and repeated doses of paraquat plus continuous exposure to normobaric 74% O2 in the breathing air for several weeks. Pulmonary fibrosis was evaluated histologically and biochemically, through the determination of to...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(85)90074-7
更新日期:1985-12-01 00:00:00
abstract::We previously reported that antibodies to squalene, an experimental vaccine adjuvant, are present in persons with symptoms consistent with Gulf War Syndrome (GWS) (P. B. Asa et al., Exp. Mol. Pathol 68, 196-197, 2000). The United States Department of Defense initiated the Anthrax Vaccine Immunization Program (AVIP) in...
journal_title:Experimental and molecular pathology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1006/exmp.2002.2429
更新日期:2002-08-01 00:00:00
abstract::Chronic heart failure (CHF) is a common disease in clinical practice, and its incidence has been increasing in recent years. Understanding the pathogenesis of CHF is the key to its future clinical diagnosis and treatment. Molecular research is a hot topic in modern hospitals, and long non-coding RNA (LncRNA) has been ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2020.104529
更新日期:2020-12-01 00:00:00