Abstract:
:To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of tissue and 42 cases of plasma samples from HCC and liver cirrhosis patients, respectively. The hypermethylation frequency of GSTP1 and RASSF1A showed significant difference between HCCs and liver cirrhosis with or without HBV infection (P<0.05), but differences of the hypermethylation status of APC, E-cadherin, and P16 were not statistically significant. There were no significant differences in the hypermethylation status of five genes between the groups of cirrhosis with and without HBV infection. The significant differences of E-cadherin, GSTP1, P16, and RASSF1A in methylation between HCCs and liver cirrhosis were not observed in the plasma samples. Furthermore, the inconsistent results of MSP and real-time quantitative PCR for the paired samples of tissue and plasma suggested that plasma DNA could not fully stand for tissue DNA. In conclusion, hypermethylation of some specific, but not all, tumor associated genes may be involved in hepatocarcinogenesis; examination of the methylation status of E-cadherin, GSTP1, P16, and RASSF1A in the plasma samples might have limited usage for HCC diagnosis.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Chang H,Yi B,Li L,Zhang HY,Sun F,Dong SQ,Cao Ydoi
10.1016/j.yexmp.2008.07.001subject
Has Abstractpub_date
2008-10-01 00:00:00pages
96-100issue
2eissn
0014-4800issn
1096-0945pii
S0014-4800(08)00084-1journal_volume
85pub_type
杂志文章abstract::We have previously described the generation of a monoclonal antibody recognizing a novel cholangiocyte marker, designated BD.1, that is expressed by fetal and adult rat cholangiocytes but not hepatocytes or the hepatic progenitor cells known as oval cells. In the present report, we have undertaken a comprehensive exam...
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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更新日期:2015-04-01 00:00:00