Abstract：
:The morphology of Gleason 4 prostate cancer (PCa) can be subdivided into cribriform and non-cribriform patterns. A large body of evidence has shown that pattern 4 cribriform PCa (especially non-glomeruloid type) is associated with adverse pathologic features and clinical outcomes compared with non-cribriform pattern 4 PCa. The underlying mechanisms for the aggressiveness of cribriform PCa are not fully understood. The aim of this study is to compare the immunohistochemical expression of various biomarkers and to determine the potential proteins that may account for their biologic and clinical differences. A total of 14 biomarkers were studied. The number of non-glomeruloid cribriform PCa cases studied for each biomarker ranged from 18 to 74 and the number of non-cribriform pattern 4 PCa studied for each biomarker ranged from 29 to 112. We demonstrated that, compared with non-cribriform Gleason pattern 4 PCa, EGFR was significantly upregulated and standard CD44 (CD44s) was significantly downregulated in cribriform PCa; no significant differences were found in the expression of AR, NKX3.1, ERG, EZH2, p53, Rb, C-Myc, BCL2, p16, CyclinD1, Her2/Neu, and Synaptophysin between these two groups of pattern 4 PCa. The study also showed, compared to non-cribriform PCa, cribriform PCa presented with significantly higher serum PSA and more advanced tumor stage. The significant overexpression of EGFR and downregulation of CD44s in non-glomeruloid cribriform PCa may, at least, partly explain the unfavorable pathology and clinical results for this growth pattern. Given that EGFR targeted inhibitors are now available, the findings may also have significant therapeutic implications.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Xiao GQ,Nguyen E,Unger PD,Sherrod AEdoi
10.1016/j.yexmp.2020.104400subject
Has Abstractpub_date
2020-06-01 00:00:00pages
104400eissn
0014-4800issn
1096-0945pii
S0014-4800(19)30877-9journal_volume
114pub_type
杂志文章abstract：:Periostin is a modular glycoprotein frequently observed to be a major constituent of the extracellular milieu of mass-forming intrahepatic cholangiocarcinoma and other desmoplastic malignant tumors. In intrahepatic cholangiocarcinoma, as well as in desmoplastic pancreatic ductal adenocarcinoma, periostin is overexpres...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2014.10.007
更新日期：2014-12-01 00:00:00
abstract：:The effect of chronic treatment with cystic fibrosis (CF) fibroblast medium on rat submandibular gland and pancreas was investigated. Rats were injected for 8 days with conditioned medium from normal or CF fibroblasts. The elemental content of the acinar cells was measured by X-ray microanalysis of cryosections. A sig...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90064-4
更新日期：1985-08-01 00:00:00
abstract：:We have previously shown that SIRT1 mRNA expression was significantly lower in relapsing MS patients compared to those in remission. Our goal was to longitudinally investigate the role of active, phosphorylated SIRT1 (p-SIRT1) as a potential biomarker of relapse and predictor for response to glatiramer acetate (GA) tr...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2018.07.008
更新日期：2018-10-01 00:00:00
abstract：:Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computat...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.104360
更新日期：2020-02-01 00:00:00
abstract：:The purpose of the study was to determine the temporal relationship between lymphocyte activating factor (LAF) activity and the acute-phase response, as measured by plasma fibronectin (Fn), C-reactive protein (CRP), and albumin levels in adjuvant arthritic rats. LAF activity as measured in the thymocyte costimulator a...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90007-x
更新日期：1988-12-01 00:00:00
abstract：:Host immune status is an important determinant of disease progression. Infections in the genital tract may alter the immunity in the particular site and hence affect the production of local cytokines. We performed this study to determine whether HIV in association with cervical HPV and CT/GC infections influences the ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2008.10.001
更新日期：2009-02-01 00:00:00
abstract：:Pentraxins belong to the superfamily of conserved proteins that are characterized by a cyclic multimeric structure. Pentraxin 3 (PTX3) is a long pentraxin which can be produced by different cell types upon exposure to various inflammatory signals. Inside the neutrophil PTX3 is stored in form of granules localized in t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.11.009
更新日期：2015-02-01 00:00:00
abstract：PURPOSE:Tumor surgery is aimed at complete resection of the lesion while ensuring a sufficient tumor-specific safety distance. Nevertheless, in many cases the most peripheral part - the invasion front - remains in situ. Tumor cells at the tumor margin have been reported to lose their epithelial properties and acquire f...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2017.02.013
更新日期：2017-04-01 00:00:00
abstract：:Heritable differences in tumor susceptibility are observed in mice after repetitive exposures to the organotropic colon carcinogen azoxymethane (AOM). The following study was undertaken to determine whether early morphological alterations within the colonic epithelium correlate with subsequent cancer risk. A/J and SWR...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/s0014-4800(99)80003-3
更新日期：1999-02-01 00:00:00
abstract：:A continuing conundrum of cancer biology is the dichotomous function of transcription factors that regulate both proliferation and apoptosis, seemingly opposite results. Previous results have indicated that regulated entry into the S-phase of the cell cycle can be anti-apoptotic. Indeed, tumor suppressor genes can be ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.09.010
更新日期：2014-12-01 00:00:00
abstract：:We studied the effects of clentiazem, a calcium channel blocker (1) on the accumulation of lipid in the aorta, (2) on the level of plasma lipids, and (3) on the number of adherent intimal monocytes and foam cells. Seventy Wistar rats were assigned to one of the following groups: (1) regular diet, (2) an atherogenic di...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(92)90010-9
更新日期：1992-12-01 00:00:00
abstract：:Certain biochemical parameters of acute liver injury induced by carbon tetrachloride were investigated in rats treated with prostacyclin (PGI2) and two of its derivatives. Serum glutamate oxalacetate transaminase elevation and both triglyceride accumulation and reduction of glycogen content in liver were significantly...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90024-3
更新日期：1985-04-01 00:00:00
abstract：:The kynurenine pathway (KP) has a principal role in the metabolism of tryptophan. This pathway is also involved in the pathogenesis of cancer. We evaluated expression of two rate limiting enzymes from this pathway (IDO1 and TDO2) as well as three long non-coding RNAs (lncRNAs) that have been predicted to alter express...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104415
更新日期：2020-06-01 00:00:00
abstract：:Histamine-N-methyltransferase, a major histamine-degrading enzyme in the skin, was purified from guinea pig skin about 150-fold. The enzymological characteristics including pH optimum, Km values for substrates, and molecular weight were almost consistent with those reported in the brain. Regulatory mechanism of the en...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(86)90014-6
更新日期：1986-12-01 00:00:00
abstract：:Chemerin is a recently described adipokine whose adipose tissue and serum levels are increased in obesity. Chemerin is expressed in the liver, and here, expression of chemerin has been studied in liver cells and in non-alcoholic fatty liver disease (NAFLD) which is more often found in obesity. Chemerin is shown to be ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2013.07.009
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Inhibition of the nuclear enzyme poly ribose synthetase (PARS) protects against in vivo lung ischemia reperfusion injury (LIRI). The effectiveness of intratracheal treatment suggests that PARS inhibition may primarily modulate alveolar macrophage (AM) activation. These studies attempted to characterize the e...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2007.11.005
更新日期：2008-04-01 00:00:00
abstract：:RNA binding motif protein 3 (RBM3) has been shown to be upregulated in several types of human tumors. Using tissue microarrays and immunohistochemistry, we showed here that both nuclear and cytoplasmic RBM3 expression levels were higher in hepatocellular carcinoma (HCC) tissues than in adjacent non-tumorous tissues. H...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104546
更新日期：2020-12-01 00:00:00
abstract：:Male rats fed a 3 g/kg copper diet were killed sequentially up to 14 weeks. Copper in liver and kidney cortex was identified histochemically and assayed in homogenates and gradient fractions following analytical subcellular fractionation on reorientating sucrose density gradients. Copper accumulated fastest in the liv...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90041-3
更新日期：1985-10-01 00:00:00
abstract：:Attempts to establish a tissue bank from autopsy samples have led to uncovering of the secrets of many diseases. Here, we examined the length of time that the RNA from postmortem tissues is available for microarray analysis and reported the gene expression profile for up- and down-regulated genes during the postmortem...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.05.007
更新日期：2016-06-01 00:00:00
abstract：:Myocardial ischemia-reperfusion (I/R) injury severely impacts the postoperative survival rate of coronary atherosclerotic heart disease. Radioprotective 105 kDa protein (RP105) is a regulator of Toll-like receptor 4 (TLR4), an inflammatory factor whose functions have been reported in myocardial I/R injury. To investig...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.12.016
更新日期：2016-04-01 00:00:00
abstract：:Soft tissue sarcomas (STSs) are comparatively rare malignant tumors with poor prognosis. STSs predominantly arise from mesenchymal differentiation. MicroRNA-34b/c, the transcriptional targets of tumor suppressor p53, possesses tumor suppressing property. Hypermethylation of miR-34b/c has been associated with tumorigen...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.03.017
更新日期：2015-06-01 00:00:00
abstract：:Fine-needle aspiration biopsy remains the mainstay for preoperative examination of thyroid nodules; however, it does not provide a definite diagnosis in up to 25% of nodules. Considerable studies have been performed to identify molecular markers to resolve this diagnostic dilemma. The aim of this study was to establis...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.08.005
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:The expression of β-catenin and paired-like homeobox 2B (PHOX2B) expression were assessed in Neuroblastoma (NB) patients as a diagnostic, prognostic and/or predictive markers. METHODS:Bone marrow (BM) samples of 52 NB patients were assessed for the expression of β-catenin by immunohistochemistry (IHC), and ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.104272
更新日期：2019-10-01 00:00:00
abstract：:As a first step in studies on the molecular mechanism(s) underlying gentamicin toxicity, the effect of treating rats with this aminoglycoside antibiotic (100 mg/kg once or twice daily for 3 days) on the analytical subfractionation of the kidney cortex has been examined. DNA was used as a marker for the nuclei, cytochr...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(87)90068-2
更新日期：1987-04-01 00:00:00
abstract：:We cloned and partially characterized a human endonuclease (Xib) which shows sequence homologies to pancreatic DNase I but an enzymatic activity closer to DNase II. We report on the structural differences found between Xib and other recently cloned human DNases. Fluores cence microscopy analysis of transiently transfe...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2254
更新日期：1999-06-01 00:00:00
abstract：BACKGROUND:DNA methylation is the most important epigenetic change involved in the control of gene expression in human cells. Methylation of the p16(INK4a) gene occurs early in the development of cervical cancer. Low-grade squamous intraepithelial lesions (LSILs) are prevalent, and their behavior is variable. OBJECTIV...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.01.007
更新日期：2015-04-01 00:00:00
abstract：:Recent studies have demonstrated important pro-inflammatory roles for two matrix metalloproteinases (MMPs)-MMP-3 (stromelysin-1) and MMP-9 (gelatinase B)-in acute lung injury [Am. J. Respir. Cell Mol. Biol. 24 (2001) 1]. A role for MMP-3 in skin inflammation has also been demonstrated [Proc. Natl. Acad. Sci. U. S. A. ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2004.01.003
更新日期：2004-06-01 00:00:00
abstract：:The most common type of liver cancer, hepatocellular carcinoma (HCC), affects over 500,000 people in the world. In the present study, liver tumor resections were used to prepare tissue arrays to examine the intensity of fluorescence of IHC stained stem cell markers in liver tissue from malignant HCC tumors and accompa...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.09.002
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVE:Annexins are a family of intracellular proteins that bind membrane phospholipids in a Ca(2+) concentration-dependent manner. Several annexins play important roles during tumor progression. However, little is known about the clinical implications and biological functions of Annexin A3 in breast cancer. METHOD...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2013.04.002
更新日期：2013-08-01 00:00:00
abstract：PURPOSE:Tumor supressor gene FHIT was identified at chromosome 3p14.2 spanning the FRA3B fragile site and is very often inactivated in different types of cancer. The aim of this study was to examine the frequency of FHIT gene LOH as well as FHIT mRNA and protein expression in sporadic colon adenocarcinoma. METHODS:The...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2013.12.005
更新日期：2014-02-01 00:00:00
