当前位置： SCI文献检索 > EXPERIMENTAL AND MOLECULAR PATHOLOGY期刊下所有文献 > Assessment of molecular testing in fine-needle aspiration biopsy samples: an experience in a Chinese population.

Assessment of molecular testing in fine-needle aspiration biopsy samples: an experience in a Chinese population.

Abstract：

:Fine-needle aspiration biopsy remains the mainstay for preoperative examination of thyroid nodules; however, it does not provide a definite diagnosis in up to 25% of nodules. Considerable studies have been performed to identify molecular markers to resolve this diagnostic dilemma. The aim of this study was to establish the distribution and frequency of common genetic alterations in a comprehensive set of benign and malignant thyroid nodules, and to determine the feasibility and role of testing for a panel of genetic alterations in improving the accuracy of cytology diagnosis in a Chinese population. This study was conducted in 314 thyroid nodules comprising 104 papillary thyroid carcinomas, 13 suspicious nodules, 52 indeterminate nodules, and 145 benign nodules. Point mutations and RET/PTC rearrangements, were evaluated by pyrosequencing and TaqMan real-time PCR, respectively. After surgery, 115 nodules were confirmed as conventional papillary thyroid carcinoma and 102 (88.70%) of these nodules harbored either the BRAF(V600E) mutation (76.52%) or RET/PTC rearrangements (12.17%). RAS mutation was found in 1 (33.33%) follicular thyroid carcinoma, 1 (14.29%) follicular thyroid adenoma and 4 (10%) goiter nodules. With cytology and molecular testing, the diagnostic accuracy was further increased to 98.82% in papillary thyroid carcinoma diagnosis, and was preoperatively increased to 76.92% and 84.00%, respectively, in nodules with suspicious and indeterminate cytology. In conclusion, molecular testing of a panel of genetic alterations in fine-needle aspiration biopsy can be effectively performed in clinical practice. It enhances the accuracy of cytology and is of particular value for indeterminate nodules in the Chinese population.

journal_name

Exp Mol Pathol

journal_title

Experimental and molecular pathology

authors

Liu S,Gao A,Zhang B,Zhang Z,Zhao Y,Chen P,Ji M,Hou P,Shi B

doi

10.1016/j.yexmp.2014.08.005

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

292-7

issue

2

eissn

0014-4800

issn

1096-0945

pii

S0014-4800(14)00131-2

journal_volume

97

pub_type

杂志文章

相关文献

EXPERIMENTAL AND MOLECULAR PATHOLOGY文献大全
  • Morphometric changes of the lung induced by inhaled bacterial endotoxin.

    abstract：:Due to the ubiquitous nature of airborne endotoxin, an understanding of pulmonary alterations which follow inhalation of environmentally realistic concentrations of purified bacteria derived lipopolysaccharide (LPS) is important. Using LPS derived from Enterobacter agglomerans, a bacterium found in cotton and cotton m...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(85)90068-1

    authors： Lantz RC,Birch K,Hinton DE,Burrell R

    更新日期：1985-12-01 00:00:00

  • Dendritic cells are abundant in non-lesional gray matter in multiple sclerosis.

    abstract：:We have analyzed the localization of dendritic cells (DCs) in non-lesional gray matter (NLGM) in comparison to non-lesional white matter (NLWM) and acute or chronic active multiple sclerosis (MS) lesions. Immunohistochemistry was performed on cryostat sections for DCs markers (CD209, CD205, CD83) and other markers for...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2007.05.006

    authors： Cudrici C,Ito T,Zafranskaia E,Niculescu F,Mullen KM,Vlaicu S,Judge SI,Calabresi PA,Rus H

    更新日期：2007-10-01 00:00:00

  • RGC-32 as a potential biomarker of relapse and response to treatment with glatiramer acetate in multiple sclerosis.

    abstract：:Currently there is critical need for the identification of reliable biomarkers to help guide clinical management of multiple sclerosis (MS) patients. We investigated the combined roles of Response Gene to Complement 32 (RGC-32), FasL, CDC2, AKT, and IL-21 as possible biomarkers of relapse and response to glatiramer ac...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2015.09.007

    authors： Kruszewski AM,Rao G,Tatomir A,Hewes D,Tegla CA,Cudrici CD,Nguyen V,Royal W 3rd,Bever CT Jr,Rus V,Rus H

    更新日期：2015-12-01 00:00:00

  • Dynamics of wound healing after silicone device implantation.

    abstract：:A large surgical wound is required for implantation of silicone mammary devices. Formation of capsules around silicone devices follows wound healing processes except that the healing is conformed and significantly delayed by the physical presence of the implant. Multilayered capsules are thicker and lymphocytic and pl...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1999.2269

    authors： Shanklin DR,Smalley DL

    更新日期：1999-09-01 00:00:00

  • Differential expression of anterior gradient protein 3 in intrahepatic cholangiocarcinoma and hepatocellular carcinoma.

    abstract：:Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer next to hepatocellular carcinoma (HCC). Despite the significant difference of the therapeutic strategy for both diseases, their histological appearance may be very similar. Thus the correct diagnosis is crucial for treatment choice bu...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.04.002

    authors： Brychtova V,Zampachova V,Hrstka R,Fabian P,Novak J,Hermanova M,Vojtesek B

    更新日期：2014-06-01 00:00:00

  • Collagen metabolism in the myocardium of normal and diabetic rats.

    abstract：:Myocardial collagen and total protein synthesis were studied in normal, diabetic, and insulin-treated diabetic rats after a single intraperitoneal injection of L-[2,3-3H]proline as a radioisotopic precursor. The incorporation of tritiated proline into myocardial protein was regarded as a measure of total protein synth...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(88)90060-3

    authors： Reddi AS

    更新日期：1988-04-01 00:00:00

  • Distribution of N-methyl-D-aspartate receptors (NMDARs) in the developing rat brain.

    abstract：:The N-methyl-D-aspartate receptor (NMDAR), which is one of the glutamate receptors, is considered to have a close relationship to synaptic plasticity in the developing brain. In addition, it is known that the excessive stimulation of NMDARs can trigger neuronal apoptosis. In this study, we examined the distribution of...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/s0014-4800(03)00030-3

    authors： Takai H,Katayama K,Uetsuka K,Nakayama H,Doi K

    更新日期：2003-08-01 00:00:00

  • Organotypic culture of human gallbladder epithelium.

    abstract：:We have modified methods of growing human gallbladder epithelial cells in monolayer and organotypic culture. These cells were grown in the presence of fetal bovine serum and with coculture of feeder layers of human gallbladder fibroblasts. Human gallbladders were obtained from cholecystectomy specimens, and the cells ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1995.1026

    authors： Oda DP,Eng L,Savard CE,Newcomer M,Haigh WG,Lee SP

    更新日期：1995-08-01 00:00:00

  • High-level microsatellite instability is not involved in gallbladder carcinogenesis.

    abstract：:The molecular alterations involved in the pathogenesis of gallbladder cancer are not yet well defined. Our aim was to determine the microsatellite status of gallbladder carcinomas and its possible correlation with alterations in K-ras and p53 genes as well as the clinicopathological characteristics of these tumors. A ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2005.04.001

    authors： Saetta AA,Gigelou F,Papanastasiou PI,Koilakou SV,Kalekou-Greca H,Miliaras D,Michalopoulos NV,Patsouris E

    更新日期：2006-02-01 00:00:00

  • The increased number of tumor-associated macrophage is associated with overexpression of VEGF-C, plays an important role in Kazakh ESCC invasion and metastasis.

    abstract：:Tumor associated macrophages (TAMs) play an important role in the growth, progression, and metastasis of tumors. The distribution of TAMs in Kazakh esophageal squamous cell carcinoma (ESCC) is not determined. We aimed to investigate the role of TAMs in the occurrence and progression of Kazakh ESCC. CD163 was used as t...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2016.12.001

    authors： Hu JM,Liu K,Liu JH,Jiang XL,Wang XL,Yang L,Chen YZ,Liu CX,Li SG,Cui XB,Zou H,Pang LJ,Zhao J,Qi Y,Liang WH,Yuan XL,Li F

    更新日期：2017-02-01 00:00:00

  • Introducing a panel for early detection of lung adenocarcinoma by using data integration of genomics, epigenomics, transcriptomics and proteomics.

    abstract：:Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computat...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2019.104360

    authors： Haghjoo N,Moeini A,Masoudi-Nejad A

    更新日期：2020-02-01 00:00:00

  • Liver necrosis induced by thyroid hormone administration in rats fed ethanol.

    abstract：:Recently it was observed that the hypothyroid state induced by propylthiouracil treatment reduced the elimination rate of ethanol. This was based on the fact that hypothyroid rats could only tolerate 10 g/kg/day of ethanol infusion over 24 h. Euthyroid rats tolerate 13 to 14 g/kg/day of ethanol infusion. In the presen...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2001.2381

    authors： Li J,French BA,Fu P,French SW

    更新日期：2001-08-01 00:00:00

  • Effect of acute plasma fibronectin depletion on tissue fibronectin levels: analysis by a new fluorescent immunoassay.

    abstract：:Plasma fibronectin modulates reticuloendothelial (RE) phagocytosis of cellular and tissue debris, fibrin microaggregates, and gelatin-coated particulates. An antigenically related, but more insoluble form of fibronectin is found in various tissues and suspected to play a role in vascular permeability, cell adhesion, a...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(84)90010-8

    authors： Saba TM,Cho E,Blumenstock FA

    更新日期：1984-08-01 00:00:00

  • RAR and RXR expression by Kupffer cells.

    abstract：:Retinoids are known to modulate macrophage differentiation, proliferation, and function including cytokine gene expression. However, signaling of retinoic acid (RA), a biologically active metabolite of vitamin A, in Kupffer cells has not been characterized. This study reports mRNA expression by rat Kupffer cells of RA...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1999.2284

    authors： Ohata M,Yamauchi M,Takeda K,Toda G,Kamimura S,Motomura K,Xiong S,Tsukamoto H

    更新日期：2000-02-01 00:00:00

  • Intestinal changes caused by DL-alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase.

    abstract：:Subacute (2 week) oral or intravenous administration of DL-alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), caused diarrhea and frequent emesis as early as 4 to 5 days in dogs (dose greater than or equal to 200 mg/kg/day). Diarrhea also occurred in monkeys after 1 week ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(83)90059-x

    authors： Yarrington JT,Sprinkle DJ,Loudy DE,Diekema KA,McCann PP,Gibson JP

    更新日期：1983-12-01 00:00:00

  • Dengue fever and bone marrow myelofibrosis.

    abstract：:Myelofibrosis is characterized by reticulin and/or collagen fibrosis in the bone marrow stroma resulting in secondary cytopenia. In addition to clonal hematologic neoplasms, myelofibrosis may also develop in association with other clinical conditions, including hematological disorders, solid malignancies, Down syndrom...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.07.004

    authors： Qing X,Sun N,Yeh J,Yue C,Cai J

    更新日期：2014-10-01 00:00:00

  • The effect of CAG repeat length polymorphism in the murine glucocorticoid receptor on transactivation potential.

    abstract：:Severe sepsis remains an important cause of death, particularly among trauma and burn patients. The severity of the systemic inflammatory response after trauma or burns and susceptibility to sepsis vary among individuals. One possible mechanism is through differential effects on glucocorticoid receptor (GR) expression...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2008.04.003

    authors： Yee KS,Cho K,Green T,Chandler J,Greenhalgh DG

    更新日期：2008-06-01 00:00:00

  • Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P.

    abstract：:Chronic heart failure (CHF) is a common disease in clinical practice, and its incidence has been increasing in recent years. Understanding the pathogenesis of CHF is the key to its future clinical diagnosis and treatment. Molecular research is a hot topic in modern hospitals, and long non-coding RNA (LncRNA) has been ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2020.104529

    authors： Li G,Du P,Qiang X,Jin D,Liu H,Li B,Guo J

    更新日期：2020-12-01 00:00:00

  • Anti-apoptotic PTD-FNK protein suppresses lipopolysaccharide-induced acute lung injury in rats.

    abstract：:The present study was aimed at clarifying the effects of an anti-apoptotic protein for modulating symptoms in acute lung injury (ALI). From Bcl-x(L), a Bcl-2 family member, we constructed an artificial protein (FNK) and fused it with the protein transduction domain (PTD) of the HIV/Tat protein (PTD-FNK) to facilitate ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2007.08.016

    authors： Chen H,Zhang L,Jin Z,Jin E,Fujiwara M,Ghazizadeh M,Asoh S,Ohta S,Kawanami O

    更新日期：2007-12-01 00:00:00

  • Exponential relationship between plasma cholesterol levels and atherosclerotic lesion size in hyperlipidemic swine.

    abstract：:The effect of fish oil supplements on atherogenesis is controversial, especially when fish oil does not lower plasma cholesterol. Some studies in swine have shown that a fish oil supplement to a butter-cholesterol diet reduces atherogenesis. The fish oil supplement also frequently reduces average plasma cholesterol le...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1993.1037

    authors： Schmee J,Kim DN,Ross JS,Thomas WA

    更新日期：1993-12-01 00:00:00

  • A time course study demonstrating RNA stability in postmortem skin.

    abstract：:Knowledge of the factors regulating the rate of mRNA degradation, including postmortem delay, is important in determining the reliability of gene expression patterns in dermal tissue. Since RNA stability can be tissue dependent, this study evaluates the effect of postmortem interval on the integrity of total RNA or th...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2006.11.001

    authors： Gopee NV,Howard PC

    更新日期：2007-08-01 00:00:00

  • EZH2 is upregulated in the proliferation centers of CLL/SLL lymph nodes.

    abstract：:Lymph node involvement of chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL) is characterised by the diffuse infiltration of small neoplastic lymphocytes, which is accompanied by the presence of proliferation centres (PCs) comprising prolymphocytes and paraimmunoblasts. There is increasing evidence of ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2018.07.009

    authors： Szurián K,Csala I,Marosvári D,Rajnai H,Dezső K,Bödör C,Piurkó V,Matolcsy A,Reiniger L

    更新日期：2018-10-01 00:00:00

  • A new model of diffuse interstitial pulmonary fibrosis in the rat.

    abstract：:We have produced experimental diffuse interstitial pulmonary fibrosis in rats with a combination of low and repeated doses of paraquat plus continuous exposure to normobaric 74% O2 in the breathing air for several weeks. Pulmonary fibrosis was evaluated histologically and biochemically, through the determination of to...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(85)90074-7

    authors： Selman M,Montaño M,Montfort I,Pérez-Tamayo R

    更新日期：1985-12-01 00:00:00

  • The effect of bezafibrate and clofibrate on cholesterol ester metabolism in rabbit peritoneal macrophages stimulated with acetylated low density lipoproteins.

    abstract：:Bezafibrate and clofibrate reduce the quantity of esterified cholesterol, decrease the incorporation of 14C-labeled oleic acid into cholesterol ester and inhibit acyl coenzyme A cholesterol acyltransferase activity in rabbit peritoneal macrophages stimulated with acetylated low density lipoprotein. In all cases, the e...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(83)90100-4

    authors： Hudson K,Mojumder S,Day AJ

    更新日期：1983-02-01 00:00:00

  • Effects of cis-diamminedichloroplatinum II (cisplatin) on the splenic tissue of rats: a histoquantitative study.

    abstract：:Histoquantitative methods were applied to study the changes of splenic structure and proportions of splenic tissue compartments in cisplatin-treated (6 mg/kg body wt) male Wistar rats. Six days after treatment the significant reduction of volume density of white pulp was observed, which was due to the very prominent d...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1994.1027

    authors： Milićević Z,Slepcević V,Nikolić D,Zivanović V,Milićević NM

    更新日期：1994-10-01 00:00:00

  • c-Yes enhances tumor migration and invasion via PI3K/AKT pathway in epithelial ovarian cancer.

    abstract：:Overexpression of c-Yes has been noted to correlation with several human cancers. However, the effects of c-Yes on epithelial ovarian cancer (EOC) development remain unclear. The aim of this study is going to prove the effects of c-Yes and related mechanisms in proliferation, metastasis and invasion of EOC. Immunohist...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2016.06.002

    authors： Jin Y,Huang M,Wang Y,Yi C,Deng Y,Chen Y,Jiang L,Wang J,Shen Q,Liu R,QinghuaXi

    更新日期：2016-08-01 00:00:00

  • Mechanisms by which acute phase proteins enhance development of liver fibrosis: effects on collagenase and prolyl-4-hydroxylase activity in the rat liver.

    abstract：:Previous experiments showed that the presence of high levels of acute phase reactants (APR) enhance CCl4-induced liver fibrosis in the rat. A high correlation was found between the degree of fibrosis and alpha 2-macroglobulin of the rat (alpha 2-macrofetoprotein, alpha M-FP) used for monitoring the acute phase respons...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(86)90056-0

    authors： van Gool J,de Nie I,Smit J,Zuyderhoudt FM

    更新日期：1986-10-01 00:00:00

  • Testicular damage by high doses of vitamin B6 (pyridoxine) in rats: a light and electron microscopical study.

    abstract：:Male Wistar rats were administered daily intraperitoneal injections of 125, 250, 500, and 1000 mg/kg/day of vitamin B6 for 2 and 6 weeks and the histogenesis of the testicular damage was investigated. A reduction of germ cells was not prominent in the 2-week groups, whereas a delay in spermiation, degeneration of elon...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(91)90019-t

    authors： Kaido M,Mori K,Ide Y,Inoue N,Koide O

    更新日期：1991-08-01 00:00:00

  • Growth and differentiation factor-5 (GDF-5) in the human intervertebral annulus cells and its modulation by IL-1ß and TNF-α in vitro.

    abstract：:Growth and differentiation factor-5 (GDF-5) is a member of the TGF-ß superfamily which regulates cell division and differentiation. GDF-5 attracted high interest because of its role in skeletal development, especially in cartilaginous sites. Little is known, however, about the role of GFD-5 in disc cell biology. The p...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.02.005

    authors： Gruber HE,Hoelscher GL,Ingram JA,Bethea S,Hanley EN Jr

    更新日期：2014-04-01 00:00:00

  • NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.

    abstract：:Oral cancer is a major health problem in many parts of the world including India. The molecular mechanisms involved in oral tumorigenesis are not completely understood. Although surgery continues to be the most common treatment modality for this cancer, survival rates of oral cancer patients have still not significant...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2006.05.001

    authors： Santhi WS,Sebastian P,Varghese BT,Prakash O,Pillai MR

    更新日期：2006-10-01 00:00:00