Abstract：
:Subacute (2 week) oral or intravenous administration of DL-alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), caused diarrhea and frequent emesis as early as 4 to 5 days in dogs (dose greater than or equal to 200 mg/kg/day). Diarrhea also occurred in monkeys after 1 week of treatment with an intravenous dose of 1000 mg/kg/day. Especially evident in the treated dogs with diarrhea were fluid loss, hemoconcentration, and decreased serum sodium and chloride which were findings totally reversible about 2 weeks after cessation of dosing. As a result of treatment with the highest intravenous dosage (1000 mg/kg/day), villous atrophy of the mucosa was observed by light and scanning electron microscopy in the canine small intestine. Transmission electron microscopy demonstrated that the most significant alterations of the canine intestinal tract involved the microvilli of epithelial cells which became shorter and were frequently less numerous or absent along focal areas of the plasma membrane. Intestinal mucosal levels of putrescine, especially in the duodenum and jejunum, were decreased as demonstrated in the monkeys following intravenous treatment with 100, 300, or 1000 mg/kg/day of DFMO. The results of this investigation are consistent with the hypothesis that the inhibition of ODC activity and subsequent altered polyamine metabolism may lead to delayed maturation of the intestinal epithelial cells and the impaired development of their microvilli, causing fluid loss due to reduced absorptive surface area.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Yarrington JT,Sprinkle DJ,Loudy DE,Diekema KA,McCann PP,Gibson JPdoi
10.1016/0014-4800(83)90059-xsubject
Has Abstractpub_date
1983-12-01 00:00:00pages
300-16issue
3eissn
0014-4800issn
1096-0945pii
0014-4800(83)90059-Xjournal_volume
39pub_type
杂志文章abstract：:The morphology of Gleason 4 prostate cancer (PCa) can be subdivided into cribriform and non-cribriform patterns. A large body of evidence has shown that pattern 4 cribriform PCa (especially non-glomeruloid type) is associated with adverse pathologic features and clinical outcomes compared with non-cribriform pattern 4...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104400
更新日期：2020-06-01 00:00:00
abstract：:Apolipoprotein E (apoE) is an important plasma protein in cholesterol homeostasis and plays a key role in the progression of glomerulosclerosis (GS). We conducted this investigation to explore whether all-trans retinoic acid (ATRA) could regulate the apoE expression in the pathological process of GS. 120 Wistar rats w...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2011.03.001
更新日期：2011-06-01 00:00:00
abstract：:In our previous study, we demonstrated that a peptide derived from the novel centrosome residing protein Cep55/c10orf3 can be targeted by the cytotoxic T lymphocytes (CTLs) in peripheral blood mononuclear cells (PBMCs) of breast carcinoma patients. In this report, we evaluated the feasibility of cancer immunotherapy u...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.10.001
更新日期：2011-02-01 00:00:00
abstract：:A large surgical wound is required for implantation of silicone mammary devices. Formation of capsules around silicone devices follows wound healing processes except that the healing is conformed and significantly delayed by the physical presence of the implant. Multilayered capsules are thicker and lymphocytic and pl...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1999.2269
更新日期：1999-09-01 00:00:00
abstract：:In the treatment of breast cancer, although a wide of choice of drugs and treatment modalities are available, drug resistance or drug toxicity poses a considerable challenge. Tranilast is a well tolerated drug used in the treatment of allergic disorders. Previous works in various models have shown that tranilast has t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.10.012
更新日期：2011-02-01 00:00:00
abstract：:There is a possibility that the aggresomes that form in the brain in neurodegenerative diseases like Alzheimer's disease (AD) and in the liver where aggresomes like Mallory-Denk Bodies (MDB) form, share mechanisms. MDBs can be prevented by feeding mice sadenosylmethionine (SAMe) or betaine. Possibly these proteins cou...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2016.03.010
更新日期：2016-06-01 00:00:00
abstract：:During short term culture of murine resident peritoneal macrophages, increasing the temperature from 37 to 39 degrees C resulted in an increased activity of several surface receptors (FcR and receptor for gluteraldehyde-fixed sheep red blood cells), enhanced phagocytosis of yeast particles, improved spreading, and an ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(91)90047-2
更新日期：1991-10-01 00:00:00
abstract：:Acute respiratory distress syndrome (ARDS) is a clinical syndrome that is characterized by diffuse alveolar damage usually secondary to an intense host inflammatory response of the lung to a pulmonary or extrapulmonary infectious or noninfectious insult. In this report we describe a unique animal model in which CBA/J ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2001.2414
更新日期：2002-02-01 00:00:00
abstract：:The keratinocyte growth factor (KGF) regulates cell growth and behavior in an autocrine or paracrine manner. In colorectal cancer tissues, KGF is expressed in tumor cells and adjacent stromal fibroblasts. We have constructed a KGF-gene-transfected cell line (HCT15-KGF) from a colorectal cancer cell line, HCT-15, that ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2007.07.001
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND:Existing clinical data have shown that high-grade prostatic intraepithelial neoplasia (HGPIN) is the most likely precursor to prostate cancer (CaP). Criteria to distinguish HGPIN that progress to CaP from those that do not remain poorly defined. Our objective was to evaluate microvessel density as a molecula...
journal_title：Experimental and molecular pathology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.yexmp.2004.04.005
更新日期：2004-10-01 00:00:00
abstract：:RAK antigens p120, p42, and p25 exhibit molecular and immunological similarity to the proteins encoded by HIV-1 and are expressed by 95% of breast and gynecological cancer cases in women and prostate cancer cases in men. Binding of the monoclonal antibody (MAb) RAK-BrI to cancer RAK antigens has been found to be inhib...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2000.2310
更新日期：2000-08-01 00:00:00
abstract：:We have previously shown that SIRT1 mRNA expression was significantly lower in relapsing MS patients compared to those in remission. Our goal was to longitudinally investigate the role of active, phosphorylated SIRT1 (p-SIRT1) as a potential biomarker of relapse and predictor for response to glatiramer acetate (GA) tr...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2018.07.008
更新日期：2018-10-01 00:00:00
abstract：:Ten cases of myopathy caused by glycogen storage diseases of type II, III, and V, and phosphorylase b kinase deficiency are reported. So-called "abnormal lysosomes" or glycogenosomes which contain abundant glycogen were found in cases of type II, and in some numbers, in cases of type III, and in one case of phosphoryl...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(83)90080-1
更新日期：1983-06-01 00:00:00
abstract：:We have previously described the generation of a monoclonal antibody recognizing a novel cholangiocyte marker, designated BD.1, that is expressed by fetal and adult rat cholangiocytes but not hepatocytes or the hepatic progenitor cells known as oval cells. In the present report, we have undertaken a comprehensive exam...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2012.04.023
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the role and regulatory mechanisms of Akt/TSC1-TSC2/mTOR signal pathway on the hepatocyte growth and proliferation after partial hepatectomy in rats. METHODS:We used the animal model of 70% hepatectomy, separated and cultivated hepatocytes. According to the different ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.01.013
更新日期：2009-04-01 00:00:00
abstract：:Pentraxins belong to the superfamily of conserved proteins that are characterized by a cyclic multimeric structure. Pentraxin 3 (PTX3) is a long pentraxin which can be produced by different cell types upon exposure to various inflammatory signals. Inside the neutrophil PTX3 is stored in form of granules localized in t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.11.009
更新日期：2015-02-01 00:00:00
abstract：:The autoimmune manifestations of MRL-+/+ (MRL/n) and MRL/Mp-lpr/lpr (MRL/l) murine models of systemic lupus erythematosus (SLE) were successfully reversed following total lymphoid irradiation (TLI) therapy consisting of 8-12 daily fractions of 200 rad. Following radiotherapy the characteristic lymphadenopathy of MRL/l...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(83)90096-5
更新日期：1983-02-01 00:00:00
abstract：:Since the first mitochondrial dysfunction was described in the 1960s, the medicine has advanced in its understanding the role mitochondria play in health, disease, and aging. A wide range of seemingly unrelated disorders, such as schizophrenia, bipolar disease, dementia, Alzheimer's disease, epilepsy, migraine headach...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2006.09.008
更新日期：2007-08-01 00:00:00
abstract：:We have produced experimental diffuse interstitial pulmonary fibrosis in rats with a combination of low and repeated doses of paraquat plus continuous exposure to normobaric 74% O2 in the breathing air for several weeks. Pulmonary fibrosis was evaluated histologically and biochemically, through the determination of to...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90074-7
更新日期：1985-12-01 00:00:00
abstract：:A broad range of cytokines are secreted during the inflammatory response by the immune system. Some cytokines promote inflammation, while others inhibit inflammation. Inflammatory cytokines work in harmony when they encounter external pathogens or internal dangers. Inflammation is resolved after the cause is eliminate...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.11.005
更新日期：2016-12-01 00:00:00
abstract：:The testicular effects of daily oral dosing with two glycol ethers--either ethylene glycol monomethyl ether (EGM) or monoethyl ether (EGE) were studied in the prepubertal rat by histological examination of the testes. Over the 11-day dosing period studied, EGM was found to produce testicular damage at dose levels of a...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(84)90074-1
更新日期：1984-04-01 00:00:00
abstract：:Several growth factors are actively synthesized during wound repair and function to stimulate different cell types involved in the process of healing. Fibrosin is a novel fibrogenic lymphokine that stimulates several biological activities that relate to in vivo scarring. To investigate the role of fibrosin, we used "p...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.2001.2402
更新日期：2001-12-01 00:00:00
abstract：:Myocardial collagen and total protein synthesis were studied in normal, diabetic, and insulin-treated diabetic rats after a single intraperitoneal injection of L-[2,3-3H]proline as a radioisotopic precursor. The incorporation of tritiated proline into myocardial protein was regarded as a measure of total protein synth...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90060-3
更新日期：1988-04-01 00:00:00
abstract：:Macrophages are crucially involved in the pathophysiology of acute pancreatitis. Soluble CD163 (sCD163) is specifically released from macrophages and systemic levels are increased in inflammatory diseases. Here, sCD163 was measured in serum of 50 patients with acute pancreatitis to find out possible associations with ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.07.006
更新日期：2015-10-01 00:00:00
abstract：PURPOSE:Tumor surgery is aimed at complete resection of the lesion while ensuring a sufficient tumor-specific safety distance. Nevertheless, in many cases the most peripheral part - the invasion front - remains in situ. Tumor cells at the tumor margin have been reported to lose their epithelial properties and acquire f...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2017.02.013
更新日期：2017-04-01 00:00:00
abstract：:As the fifth most common cancer and the second leading cause of cancer related deaths worldwide, hepatocellular carcinoma (HCC) causes up to one million deaths annually. Alcoholic steatohepatitis (ASH) and non-alcoholic steatohepatitis (NASH) are becoming the two major risk factors because both may develop liver fibro...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.04.002
更新日期：2019-06-01 00:00:00
abstract：:The kynurenine pathway (KP) has a principal role in the metabolism of tryptophan. This pathway is also involved in the pathogenesis of cancer. We evaluated expression of two rate limiting enzymes from this pathway (IDO1 and TDO2) as well as three long non-coding RNAs (lncRNAs) that have been predicted to alter express...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104415
更新日期：2020-06-01 00:00:00
abstract：:As the number of elderly patients increases, some patients with heart problems may also need surgery. The purpose of this study was to investigate whether dexmedetomidine (DEX), a common used anesthetic, was beneficial to the patients with heart problems. Myocardial cells induced by doxorubicin (DOX) was to simulate t...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104371
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND:Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to be critical in the onset and progression of acute myocardial ischemia (AMI). This study attempted to reveal the biological function of MALAT1 in AMI. METHODS:Expression of MALAT1 in H9c2 cells was silenced by shRNA-mediated...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.104325
更新日期：2019-12-01 00:00:00
abstract：:We have modified methods of growing human gallbladder epithelial cells in monolayer and organotypic culture. These cells were grown in the presence of fetal bovine serum and with coculture of feeder layers of human gallbladder fibroblasts. Human gallbladders were obtained from cholecystectomy specimens, and the cells ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1026
更新日期：1995-08-01 00:00:00
