Abstract：
:Retinoids are known to modulate macrophage differentiation, proliferation, and function including cytokine gene expression. However, signaling of retinoic acid (RA), a biologically active metabolite of vitamin A, in Kupffer cells has not been characterized. This study reports mRNA expression by rat Kupffer cells of RA receptor (RAR) and retinoid X receptor (RXR) subtypes and their binding activities to the RA responsive element (RARE) or retinoid X responsive element (RXRE). Total RNA and nuclear proteins were extracted from Kupffer cells immediately following isolation from livers of normal male Wistar rats. Competitive PCR demonstrated relative mRNA expression of RAR and RXR subtypes in the order of beta>alpha>gamma for and alpha>beta>gamma, respectively. It also demonstrated that the RXR alpha and beta mRNA levels were 5- to 10-fold higher in Kupffer cells than in hepatic stellate cells while RAR mRNA expression was shown to be similar for all the subtypes in both cell types. Gel mobility shift assays of nuclear extracts with labeled RARE and RXRE probes showed distinct binding activities for both responsive elements, which were effectively displaced with cold probes in excess but not with an unrelated oligonucleotide. A supershift assay with an antibody against RARalpha or RXRalpha has confirmed the contribution of both receptors to RARE binding and that of the RXRalpha to RXRE binding activity. These results represent the first demonstration of RA signaling at the nuclear level in Kupffer cells.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Ohata M,Yamauchi M,Takeda K,Toda G,Kamimura S,Motomura K,Xiong S,Tsukamoto Hdoi
10.1006/exmp.1999.2284keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
13-20issue
1eissn
0014-4800issn
1096-0945pii
S0014-4800(99)92284-0journal_volume
68pub_type
杂志文章abstract：:Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computat...
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更新日期：2014-02-01 00:00:00
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更新日期：2019-08-01 00:00:00
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journal_title：Experimental and molecular pathology
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doi：10.1006/exmp.1994.1037
更新日期：1994-12-01 00:00:00
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更新日期：1986-02-01 00:00:00
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journal_title：Experimental and molecular pathology
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doi：10.1016/j.yexmp.2013.07.009
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pub_type： 杂志文章
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更新日期：1988-06-01 00:00:00
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journal_title：Experimental and molecular pathology
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更新日期：1993-02-01 00:00:00
abstract：:Thrombospondin 2 (TSP2) is a protein with important roles in different tumor types, mainly related to tumor inhibition. However, there are limiting data regarding TSP2 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). We aimed to investigate TSP2 transcript and protein expression in tumoral and non-tumo...
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