Abstract:
:Several laboratories have recently reported on the structural and functional characteristics of an LDL fraction isolated from atherosclerotic lesions, designated A-LDL. Given the wide variety of tissue sources and isolation conditions that have been employed, we have addressed whether several procedures currently used affect the interaction of A-LDL with macrophages, and, if so, by what mechanisms. We isolated A-LDL from human aortic plaques by ultracentrifugation and gel filtration chromatography. Although some differences in the chromatographic elution profiles on gel filtration were apparent between homogenized and nonhomogenized extracts, A-LDL isolated from the same pool of plaque minces with or without homogenization showed no differences in macrophage degradation or inhibition of this degradation by excess acetyl-LDL. A-LDL isolated from plaques obtained at surgery or at autopsy less than 12 hr after death also showed no major differences in macrophage recognition, suggesting that post-mortem changes were probably not affecting cell recognition. However, A-LDL particles underwent aggregation when subjected to concentration, when stored for periods of 2 weeks or more, or when subjected to vortexing. The aggregated A-LDL was degraded more readily by macrophages than unaggregated A-LDL, and inhibition of degradation of aggregated A-LDL by excess acetyl-LDL was less than for unaggregated A-LDL. Collectively, these studies show that although post-mortem changes and tissue homogenization do not appreciably affect the interaction of A-LDL with macrophages in culture, other isolation and preparation conditions have dramatic effects which could explain some of the diversity of A-LDL metabolism reported in the literature.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Hoff HF,O'Neil J,Cole TBdoi
10.1016/0014-4800(91)90045-ysubject
Has Abstractpub_date
1991-02-01 00:00:00pages
72-86issue
1eissn
0014-4800issn
1096-0945pii
0014-4800(91)90045-Yjournal_volume
54pub_type
杂志文章abstract::Ureaplasma urealyticum is a common inhabitant of mucosal surfaces but is also associated with a higher incidence of pneumonia and bronchopulmonary dysplasia in preterm infants. Culture and polymerase chain reaction demonstrate high isolation rates of ureaplasma in clinical specimens documenting their presence but do n...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/s0014-4800(03)00051-0
更新日期:2003-10-01 00:00:00
abstract::We tested the hypothesis that structural changes in the genome parallel age- and organ-specific phenotypes in conjunction with the differential transposition activities of retroelements. The genomes of the liver from C57BL/6J mice were larger than other organs, coinciding with an increase in genomic copies of certain ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2012.04.013
更新日期:2012-08-01 00:00:00
abstract::Megalencephaly-polymicrogyria-polydactyly-hydrocephalus (MPPH) syndrome is a developmental brain disorder characterized by an enlarged brain size with bilateral perisylvian polymicrogyria and a variable degree of ventriculomegaly. MPPH syndrome is associated with oromotor dysfunction, epilepsy, intellectual disability...
journal_title:Experimental and molecular pathology
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doi:10.1016/j.yexmp.2020.104471
更新日期:2020-08-01 00:00:00
abstract::Piecemeal necrosis, currently called interface hepatitis, is a feature of viral hepatitis as well as autoimmune hepatitis and steatohepatitis. The mechanism of liver cell loss and piecemeal necrosis needs to be determined. We hypothesize that piecemeal necrosis in hepatitis is due to a piecemeal removal of hepatocyte ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.2001.2397
更新日期:2001-10-01 00:00:00
abstract::Ketamine, a common N-methyl-d-aspartate receptor (NMDAR) antagonist, is an option for cancer pain treatment in clinical practice. Ketamine has been shown to have the capacity to attenuate cancer cells malignancy. However, the underlying mechanism remains elusive. In the present study, we reported that ketamine inhibit...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.02.004
更新日期:2019-04-01 00:00:00
abstract::This paper is based upon the "Charles Lieber Satellite Symposia" organized by Manuela G. Neuman at the Research Society on Alcoholism (RSA) Annual Meetings, 2013 and 2014. The present review includes pre-clinical, translational and clinical research that characterize alcoholic liver disease (ALD) and non-alcoholic ste...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2014.09.005
更新日期:2014-12-01 00:00:00
abstract::CD44 is a polymorphic family of immunologically related integral membrane glycoproteins associated with cell matrix adhesion, lymphocyte activation and targeting, and tumor growth and metastasis. We studied CD44 expression in 114 formalin-fixed paraffin-embedded thyroid tumors using the A3D8 anti-human CD44 monoclonal...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1037
更新日期:1994-12-01 00:00:00
abstract::Although clinical diabetes mellitus is obviously a high risk factor for myocardial infarction, there is disagreement about the sensitivity of ischemic injury of an infarcted myocardium in experimental studies. The present study evaluated the influences of different durations of hyperglycemia on ischemic and reperfusio...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2003.08.003
更新日期:2004-02-01 00:00:00
abstract::To study the effects of homocysteine on epithelial and stromal tissues, the free base of homocysteine thiolactone was synthesized and administered to mice. Intraperitoneal and intramuscular doses of 0.2 to 2.0 mg/g are acutely toxic, causing as much as 90% mortality because of intense tissue necrosis at the injection ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(89)90016-6
更新日期:1989-10-01 00:00:00
abstract::The hypoxia-inducible factor-1alpha (HIF-1alpha) is expressed in response to hypoxia and has been recently demonstrated in a variety of cells such as tumor cells and tumor-associated macrophages. Several characteristics of leishmanial lesions in humans and in animal models, such as microcirculation impairment, metabol...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.09.002
更新日期:2005-02-01 00:00:00
abstract::The relative resistance of fibroblasts to hypoxia and their remarkable adaptive plasticity in response to rapid changes in local tissue microenvironment made interstitial cardiac fibroblasts to be a key player in post-myocardial infarction myocardial repair. Cardiac fibroblasts are abundantly presented in the intersti...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2016.09.002
更新日期:2016-10-01 00:00:00
abstract::Smooth muscle cells from monkey aorta quiescent in 5% calf serum have been shown to be stimulated to renewed proliferation by hyperlipidemic serum or LDL from such serum. This proliferative response evidently is not dependent on platelet-derived growth factor present in our system in large quantities. The least exposu...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(88)90043-3
更新日期:1988-02-01 00:00:00
abstract::Conjunctival melanocytic lesions encompass a group of clinically diverse, benign to malignant, neoplasms that may contain overlapping histopathological features, making definitive diagnosis challenging in some cases. In this series, we compared multiple immunohistochemical (IHC) markers in 11 conjunctival nevi, 10 pri...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2017.01.016
更新日期:2017-04-01 00:00:00
abstract::Although the extraction and analysis of nucleic acids from formalin-fixed paraffin-embedded tissues is a routine and growing part of pathology practice, no generally accepted recommendations exist to guide laboratories in their selection of tissue fixation, processing and DNA/RNA extraction techniques. The aim of this...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2011.09.013
更新日期:2012-02-01 00:00:00
abstract:OBJECT:Mutations of the gene encoding isocitrate dehydrogenase (IDH) have been shown in a significant proportion of diffuse gliomas. These mutations are specific to gliomas and their utility for diagnosis and prognostication of these tumors is being proclaimed. The present study was conducted with the aim of assessing ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2011.04.017
更新日期:2011-08-01 00:00:00
abstract::Male Wistar rats were administered daily intraperitoneal injections of 125, 250, 500, and 1000 mg/kg/day of vitamin B6 for 2 and 6 weeks and the histogenesis of the testicular damage was investigated. A reduction of germ cells was not prominent in the 2-week groups, whereas a delay in spermiation, degeneration of elon...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(91)90019-t
更新日期:1991-08-01 00:00:00
abstract::The purpose of the present study was to evaluate the relationship between class II major histocompatibility complex (MHC) genes (HLA-DR and HLA-DQB) and the genetic susceptibility to idiopathic dilated cardiomyopathy (IDC) in Mexican patients. The HLA-DR and DQB alleles were analyzed in 53 patients with IDC and 99 eth...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2006.10.002
更新日期:2007-02-01 00:00:00
abstract::Ten cases of myopathy caused by glycogen storage diseases of type II, III, and V, and phosphorylase b kinase deficiency are reported. So-called "abnormal lysosomes" or glycogenosomes which contain abundant glycogen were found in cases of type II, and in some numbers, in cases of type III, and in one case of phosphoryl...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(83)90080-1
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND:Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to be critical in the onset and progression of acute myocardial ischemia (AMI). This study attempted to reveal the biological function of MALAT1 in AMI. METHODS:Expression of MALAT1 in H9c2 cells was silenced by shRNA-mediated...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.104325
更新日期:2019-12-01 00:00:00
abstract::Kidney aging has been recognized as a chronic process of compromised renal function and structural changes in the tubulointerstitium and glomerulus. Cell senescence is associated with alterations in cell structure and function, including expression of cytokines and structural and regulatory components of extracellular...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.2000.2346
更新日期:2001-02-01 00:00:00
abstract::Experimental autoimmune myositis (EAM) is a rodent model for human inflammatory muscle disease (IMD). It can be induced by immunization of rodents with skeletal muscle homogenate and adjuvant. The specific myositogenic autoantigen has not been clearly identified although some evidence points to skeletal muscle myosin....
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/s0014-4800(03)00003-0
更新日期:2003-06-01 00:00:00
abstract::Spiroplasma spp. are important phyto and insect pathogens, and candidate causal agent/s of transmissible spongiform encephalopathies (TSE) in man and animals. These filterable wall-less bacteria are widely distributed in nature with an unspecified environmental reservoir. In this study we showed by scanning electron m...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2012.04.017
更新日期:2012-08-01 00:00:00
abstract::Dendritic cells (DCs) are a subset of antigen-presenting cells (APCs) that are involved in the initiation and control of the immune response to antigens present at the interface with the environment. A limited number of groups have studied DCs in human and animal conjunctiva but no data is available concerning the dif...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression was reported in various kinds of tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in pancrea...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.01.010
更新日期:2010-06-01 00:00:00
abstract::We have modified methods of growing human gallbladder epithelial cells in monolayer and organotypic culture. These cells were grown in the presence of fetal bovine serum and with coculture of feeder layers of human gallbladder fibroblasts. Human gallbladders were obtained from cholecystectomy specimens, and the cells ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1995.1026
更新日期:1995-08-01 00:00:00
abstract::The aim of this study is to determine if warm ischemia after surgical extirpation impacts gene expression in tissue samples which will be used for cDNA array analysis. We investigated effects of warm ischemia on gene expression in lung, liver, kidney, and spleen of rats, chronologically, using an original cDNA array, ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.07.004
更新日期:2004-12-01 00:00:00
abstract::Parkinson's disease (PD) is a chronic neurodegenerative disease characterized by loss of dopaminergic neurons in the substantia nigra. Recently, microRNAs (miRNAs) were emerging as important mediators in dopaminergic neuron biology. This study determined miR-410 expression in the 6-hydroxydopamine (6-OHDA)-induced in ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.05.002
更新日期:2019-08-01 00:00:00
abstract::Recent research has shown that oncogenic human papillomavirus (HPV) DNA, which is currently used in the screening and diagnosis of cervical cancer, can be detected not only in high-grade cervical lesions, but also in low-grade cervical lesions and normal tissues. For this reason, HPV tests targeting the E6 and E7 mRNA...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2014.08.004
更新日期:2014-10-01 00:00:00
abstract::Myocardial collagen and total protein synthesis were studied in normal, diabetic, and insulin-treated diabetic rats after a single intraperitoneal injection of L-[2,3-3H]proline as a radioisotopic precursor. The incorporation of tritiated proline into myocardial protein was regarded as a measure of total protein synth...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(88)90060-3
更新日期:1988-04-01 00:00:00
abstract::RAK antigens p120, p42, and p25 exhibit molecular and immunological similarity to the proteins encoded by HIV-1 and are expressed by 95% of breast and gynecological cancer cases in women and prostate cancer cases in men. Binding of the monoclonal antibody (MAb) RAK-BrI to cancer RAK antigens has been found to be inhib...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.2000.2310
更新日期:2000-08-01 00:00:00