Abstract:
:Ureaplasma urealyticum is a common inhabitant of mucosal surfaces but is also associated with a higher incidence of pneumonia and bronchopulmonary dysplasia in preterm infants. Culture and polymerase chain reaction demonstrate high isolation rates of ureaplasma in clinical specimens documenting their presence but do not associate the organism directly with the diseased tissue. In this study, lung tissue samples from newborn mice inoculated intranasally with U. urealyticum were used to develop an in situ hybridization (ISH) test for the organism. In situ hybridization allows the localization of gene expression for visualization within the context of tissue morphology. New techniques which use biotinyl-tyramide based signal amplification have been able to greatly enhance the sensitivity of ISH. Using the Dako GenPoint Catalyzed Signal Amplification system to detect a biotinylated DNA probe specific for an internal nucleotide sequence within the urease gene of U. urealyticum, the organism was detected within the infected murine lung tissues. Electron microscopy was used to verify the presence of the organisms in the positive ISH areas. The ISH procedure developed in this study can be used to analyze the presence of ureaplasma in human neonatal lung tissue with the corresponding histopathology.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Benstein BD,Crouse DT,Shanklin DR,Ourth DDdoi
10.1016/s0014-4800(03)00051-0keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
165-70issue
2eissn
0014-4800issn
1096-0945pii
S0014480003000510journal_volume
75pub_type
杂志文章abstract:OBJECT:Mutations of the gene encoding isocitrate dehydrogenase (IDH) have been shown in a significant proportion of diffuse gliomas. These mutations are specific to gliomas and their utility for diagnosis and prognostication of these tumors is being proclaimed. The present study was conducted with the aim of assessing ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2011.04.017
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abstract::Mucoepidermoid carcinoma (MEC) is the most prevalent malignant tumor in major and minor salivary glands (SGs). We have recently identified human cytomegalovirus (hCMV) as a principle component in the multifactorial causation of SG-MEC. This finding is corroborated by the ability of the purified mouse CMV (mCMV) to ind...
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abstract::A large surgical wound is required for implantation of silicone mammary devices. Formation of capsules around silicone devices follows wound healing processes except that the healing is conformed and significantly delayed by the physical presence of the implant. Multilayered capsules are thicker and lymphocytic and pl...
journal_title:Experimental and molecular pathology
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doi:10.1006/exmp.1999.2269
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(91)90046-z
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abstract::The glycosaminoglycan (GAG) contents of hypertrophic scars, normal scars, and human skin from cadavers of matched ages were compared. Cellulose acetate electrophoresis, chondroitinase digestions, and reaction product and infrared analyses were used to characterize the component GAGs. DEAE-cellulose chromatography was ...
journal_title:Experimental and molecular pathology
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(92)90043-b
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1035
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doi:10.1016/j.yexmp.2019.04.002
更新日期:2019-06-01 00:00:00
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journal_title:Experimental and molecular pathology
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abstract::The Chediak-Higashi syndrome (CHS) is an inherited disorder of humans and of several animal species, characterized by partial albinism, pseudohemophilia, increased susceptibility to disease, and large inclusions in all granule-forming cells. In this study, various parameters of adenine nucleotide biochemistry were exa...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1993.1004
更新日期:1993-02-01 00:00:00
abstract:BACKGROUND:The expression of β-catenin and paired-like homeobox 2B (PHOX2B) expression were assessed in Neuroblastoma (NB) patients as a diagnostic, prognostic and/or predictive markers. METHODS:Bone marrow (BM) samples of 52 NB patients were assessed for the expression of β-catenin by immunohistochemistry (IHC), and ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.104272
更新日期:2019-10-01 00:00:00
abstract::The influence of Kupffer cells (KC) from normal, D-galactosamine- and thioacetamide-injured liver on the growth of rat liver fat-storing cells (FSC) in culture was studied. The supplementation of FSC incubation medium with normal and galactosamine-KC-conditioned media for 4 days caused 1.5- and 1.9-fold increase (P le...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(88)90023-8
更新日期:1988-08-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1995.1040
更新日期:1995-12-01 00:00:00
abstract::We have previously reported the mechanism behind the myocardial injury and the activation of autonomic nervous system during the ischemia-reperfusion (IR) of the rat brain. This study was planned to investigate the effect of carvedilol, a β-blocker, in improving the myocardial injury caused by IR of the rat brain. We ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2015.04.001
更新日期:2015-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.01.002
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pub_type: 杂志文章,收录出版
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2006.01.004
更新日期:2006-12-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(92)90037-c
更新日期:1992-06-01 00:00:00
abstract::Retinoids influence the pathogenesis of alcohol liver disease (ALD). To analyze the impact of retinoid X receptor alpha (RXRalpha) on ALD, alcohol-induced hepatotoxicity was studied using mice fed ethanol intragastrically for 25 days. Alcohol-induced microvesicular fat around the central vein and drug-induced morpholo...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/s0014-4800(03)00091-1
更新日期:2003-12-01 00:00:00
abstract::Partial-thickness skin burns have been shown to induce neutrophil-dependent microvascular injury both locally (skin) and systemically (lung). In the present study, interventional measures to block inflammatory chemoattractants were employed to define the pathophysiologic role of these mediators in the development of s...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1999.2263
更新日期:1999-08-01 00:00:00
abstract::Growth and differentiation factor-5 (GDF-5) is a member of the TGF-ß superfamily which regulates cell division and differentiation. GDF-5 attracted high interest because of its role in skeletal development, especially in cartilaginous sites. Little is known, however, about the role of GFD-5 in disc cell biology. The p...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2014.02.005
更新日期:2014-04-01 00:00:00
abstract::Smooth muscle cells from monkey aorta quiescent in 5% calf serum have been shown to be stimulated to renewed proliferation by hyperlipidemic serum or LDL from such serum. This proliferative response evidently is not dependent on platelet-derived growth factor present in our system in large quantities. The least exposu...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(88)90043-3
更新日期:1988-02-01 00:00:00
abstract::Sabaeksan has been used for prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of Sabaeksan, we examined the effect of Sabaeksan on the phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187-induced pro-inflammatory cytokines secretion in human m...
journal_title:Experimental and molecular pathology
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doi:10.1016/j.yexmp.2005.01.001
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abstract::The purpose of this study was to better establish the morphologic basis of the early mucosal lesions of experimental inflammatory bowel disease because understanding the development of these lesions has important pathogenetic implications. Sprague-Dawley rats were given 1.5% hydrolyzed lambda-carrageenan orally for 30...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1994.1011
更新日期:1994-04-01 00:00:00
abstract::Male Wistar rats were administered daily intraperitoneal injections of 125, 250, 500, and 1000 mg/kg/day of vitamin B6 for 2 and 6 weeks and the histogenesis of the testicular damage was investigated. A reduction of germ cells was not prominent in the 2-week groups, whereas a delay in spermiation, degeneration of elon...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(91)90019-t
更新日期:1991-08-01 00:00:00