Abstract:
:The influence of Kupffer cells (KC) from normal, D-galactosamine- and thioacetamide-injured liver on the growth of rat liver fat-storing cells (FSC) in culture was studied. The supplementation of FSC incubation medium with normal and galactosamine-KC-conditioned media for 4 days caused 1.5- and 1.9-fold increase (P less than 0.005), respectively, in the DNA contents of FSC on the fifth day of culture. The stimulant effect reached a maximum at a 1:2 dilution of the conditioned medium with FSC incubation medium. The population doubling time of FSC grown in the absence of conditioned medium was calculated to be 41.5 +/- 3 hr; it was greatly shortened by KC-conditioned medium from galactosamine-treated rats (24 +/- 2 hr), by normal KC-conditioned medium (28 +/- 4 hr), and by KC medium from thioacetamide-injured rats (33 +/- 5 hr). Similarly KC-conditioned media of either source stimulated significantly the rate of [3H]thymidine incorporation into DNA of FSC even during short-term exposures of FSC with KC medium. The growth-promoting effect of normal KC-conditioned medium could be enhanced if the Kupffer cells were challenged with zymosan and phorbol esters, respectively, but not with lipopolysaccharide. The light microscopic appearance of FSC grown in the presence of KC-conditioned media was greatly changed: the cultures became more dense; the cells spread and developed long extensions. The size and number of lipid droplets decreased more rapidly than in control cultures maintained without addition of KC-conditioned media. The protein DNA ratio of FSC exposed with KC-conditioned media was reduced due to a strong increase in DNA, which is not followed by a similar increase in cellular protein. If referred to the DNA content of the culture, the incorporation of [3H]valine into the protein of the medium was not changed, that into cellular protein was strongly reduced under the influence of normal KC-conditioned medium. Secretions of Kupffer cells obviously stimulate significantly the proliferation of FSC in culture.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Zerbe O,Gressner AMdoi
10.1016/0014-4800(88)90023-8subject
Has Abstractpub_date
1988-08-01 00:00:00pages
87-101issue
1eissn
0014-4800issn
1096-0945pii
0014-4800(88)90023-8journal_volume
49pub_type
杂志文章abstract:PURPOSE:Tumor supressor gene FHIT was identified at chromosome 3p14.2 spanning the FRA3B fragile site and is very often inactivated in different types of cancer. The aim of this study was to examine the frequency of FHIT gene LOH as well as FHIT mRNA and protein expression in sporadic colon adenocarcinoma. METHODS:The...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2013.12.005
更新日期:2014-02-01 00:00:00
abstract::Primary hepatic peripheral T-cell lymphoma (PTCL) is exceedingly rare. We encountered such a case in a 58-year-old Hispanic female with a history of chronic sinusitis and hypothyroidism who presented with 4weeks of fever and weight loss. Laboratory studies showed altered liver function and mild pancytopenia. Hepatitis...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
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abstract::Subacute (2 week) oral or intravenous administration of DL-alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), caused diarrhea and frequent emesis as early as 4 to 5 days in dogs (dose greater than or equal to 200 mg/kg/day). Diarrhea also occurred in monkeys after 1 week ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(83)90059-x
更新日期:1983-12-01 00:00:00
abstract::In vivo and in vitro studies performed on the polar solvent N-methylformamide (NMF), as well as on its association with chemotherapeutic agents or X rays, have clearly demonstrated that this compound is capable of inducing changes in biological characteristics of tumor cells, e.g., cell differentiation. However, the m...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(85)90031-0
更新日期:1985-04-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2004.01.002
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Galectin-3 is a lectin that presents pivotal roles in tumor biology and there are no studies evaluating their expression in dysplasias and carcinomas developed from tongue carcinogenesis models. AIMS:To investigate the role of galectin-3 in the development of tongue carcinomas using a mouse model of oral ca...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.12.007
更新日期:2011-04-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(83)90103-x
更新日期:1983-02-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2015.04.001
更新日期:2015-06-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(84)90074-1
更新日期:1984-04-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(89)90027-0
更新日期:1989-04-01 00:00:00
abstract::IL-12Rβ2(-/-) mice, which are unresponsive to IL-12, develop severe experimental autoimmune encephalomyelitis (EAE). The mechanisms for enhanced autoimmunity are incompletely understood. We report that in IL-12Rβ2(-/-) mice, thymocytes undergo markedly accelerated maturation. This occurs at the transition from a doubl...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2010.06.003
更新日期:2010-10-01 00:00:00
abstract::Integrins are a family of cell surface glycoproteins that act as receptors for ECM proteins or for membrane bound counter-receptors on other cells. The integrin receptor family of vertebrates includes at least 16 distinct alpha subunits and at least 8 beta subunits which can associate to form more than 20 distinct int...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2005.04.004
更新日期:2005-08-01 00:00:00
abstract::Lymph node involvement of chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL) is characterised by the diffuse infiltration of small neoplastic lymphocytes, which is accompanied by the presence of proliferation centres (PCs) comprising prolymphocytes and paraimmunoblasts. There is increasing evidence of ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2018.07.009
更新日期:2018-10-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(86)90045-6
更新日期:1986-06-01 00:00:00
abstract::Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.2002.2470
更新日期:2002-12-01 00:00:00
abstract::The present study was aimed at clarifying the effects of an anti-apoptotic protein for modulating symptoms in acute lung injury (ALI). From Bcl-x(L), a Bcl-2 family member, we constructed an artificial protein (FNK) and fused it with the protein transduction domain (PTD) of the HIV/Tat protein (PTD-FNK) to facilitate ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.08.016
更新日期:2007-12-01 00:00:00
abstract::Fluoride is known to cause ectopic calcification. The biochemical mechanism(s) involved in the initiation of calcification is not understood and the accompanying ultrastructural changes remain to be elucidated. Therefore, certain relevant parameters have been investigated in the aorta of rabbits administered fluoride,...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(90)90025-9
更新日期:1990-08-01 00:00:00
abstract::Previous experiments showed that the presence of high levels of acute phase reactants (APR) enhance CCl4-induced liver fibrosis in the rat. A high correlation was found between the degree of fibrosis and alpha 2-macroglobulin of the rat (alpha 2-macrofetoprotein, alpha M-FP) used for monitoring the acute phase respons...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(86)90056-0
更新日期:1986-10-01 00:00:00
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journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2007.05.002
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:To identify the relationship between clinical pathological characteristics and the recurrence score (RS) on a 21-gene expression assay in patients with hormone receptor-positive, node-negative breast cancer, as well as the effect of RS on adjuvant decision-making. METHODS:The retrospective study was conducte...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2019.04.013
更新日期:2019-06-01 00:00:00
abstract::The maintenance of homeostasis of the immune system depends on the balance between excitatory and inhibitory signals. Programmed death ligand (PD-L1) is a molecule which downregulates the immune system targeting the programmed death receptor 1 (PD-1). Under physiological conditions, the receptor is constitutively expr...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2020.104494
更新日期:2020-10-01 00:00:00
abstract::The pathogenesis of skeletal muscle necrosis induced by crude Bothrops asper venom and isolated myotoxic phospholipase was studied using light and electron microscopy. White mice were injected intramuscularly with a dose of 2.5 micrograms/g and tissue samples were taken at 30 min and 1, 3, 6, 12, 24, and 48 hr. Toxin-...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/0014-4800(84)90054-6
更新日期:1984-06-01 00:00:00
abstract::Previous studies demonstrated that the anti-phosphorylcholine (PC) antibodies produced by aged BALB/c (18-22 months old) mice are structurally different and less protective against infection with Streptococcus pneumoniae than those produced by young (3-4 months old) syngeneic mice. However, PC antibody from aged anima...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.1995.1011
更新日期:1995-04-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR)1 and 2 signaling is a potent activator of tumor angiogenesis. Although the expressions of VEGFR1 and VEGFR2 were initially thought to be limited to the endothelial cells, it is now known that both the receptors are expressed in tumor cells. This is the fi...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/j.yexmp.2020.104408
更新日期:2020-06-01 00:00:00
abstract::Ureaplasma urealyticum is a common inhabitant of mucosal surfaces but is also associated with a higher incidence of pneumonia and bronchopulmonary dysplasia in preterm infants. Culture and polymerase chain reaction demonstrate high isolation rates of ureaplasma in clinical specimens documenting their presence but do n...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/s0014-4800(03)00051-0
更新日期:2003-10-01 00:00:00
abstract::To assess elastin biosynthesis in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model. The time points of the study were 1, 3, and 7 days and 2, 4, and 8 weeks after coarctation. Additional animals were...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1006/exmp.2002.2423
更新日期:2002-04-01 00:00:00
abstract::TNF-alpha is a proinflammatory cytokine, abundantly expressed after myocardial infarction. It has been suggested that it exhibits myocardial suppressive and cytotoxic effects. AG-556 is a tyrosine kinase inhibitor synthesized based on its ability to reduce TNF-alpha production and cell toxicity, and to improve experim...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章
doi:10.1016/s0014-4800(03)00022-4
更新日期:2003-06-01 00:00:00
abstract::Autoimmune thyroid disease (AITD) is a complex disorder with both genetic and environmental risk factors. A number of genetic factors such as HLA and CTLA-4 loci have been associated with risk of this disorder. In addition to these factors, recent studies have shown contribution of non-coding RNAs in the pathogenesis ...
journal_title:Experimental and molecular pathology
pub_type: 杂志文章,评审
doi:10.1016/j.yexmp.2020.104527
更新日期:2020-12-01 00:00:00