Quantitative assessment and clinical relevance of VEGFRs-positive tumor cells in refractory brain tumors.

Abstract:

:Vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR)1 and 2 signaling is a potent activator of tumor angiogenesis. Although the expressions of VEGFR1 and VEGFR2 were initially thought to be limited to the endothelial cells, it is now known that both the receptors are expressed in tumor cells. This is the first study wherein VEGFRs-positive tumor cells are quantitatively evaluated for brain tumors with upregulated VEGF/VEGFR signaling. The percentage of VEGFRs-positive tumor cells was quantitatively evaluated in various brain tumors (10 glioblastomas, 22 neurofibromatosis type 2 [NF2]-related schwannomas, 21 sporadic schwannomas, 27 chordomas, 36 meningiomas, 29 hemangioblastomas, 11 hemangiopericytoma, and 13 ependymomas) using immunohistochemistry. VEGF-A expression was also analyzed using quantitative real-time polymerase chain reaction. Double immunofluorescence staining using anti-PDGFR-β and anti-CD34 antibody, microvessel density, and vessel diameter were analyzed to evaluate the vascular characteristics. Chordomas demonstrated an extremely higher percentage of VEGFR1 and VEGFR2-positive tumor cells than other tumors. In contrast, meningiomas and hemangiopericytomas showed few VEGFRs-positive tumor cells. The percentage of positive tumor cells in chordomas, hemangioblastomas, and NF2 schwannomas was associated with clinical courses, such as shorter progression free survival, and growth speed. Glioblastomas and NF2 schwannomas showed larger tumor vessels without pericyte coverage. The present study is the first to quantitatively analyze VEGFR1- and VEGFR2- positive tumor cells in various types of refractory brain tumors. This novel parameter significantly correlated with the progressive clinical courses.

journal_name

Exp Mol Pathol

authors

Tamura R,Sato M,Morimoto Y,Ohara K,Kosugi K,Oishi Y,Kuranari Y,Murase M,Yoshida K,Toda M

doi

10.1016/j.yexmp.2020.104408

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

104408

eissn

0014-4800

issn

1096-0945

pii

S0014-4800(19)30909-8

journal_volume

114

pub_type

杂志文章
  • Glycosaminoglycans of normal and hypertrophic human scar.

    abstract::The glycosaminoglycan (GAG) contents of hypertrophic scars, normal scars, and human skin from cadavers of matched ages were compared. Cellulose acetate electrophoresis, chondroitinase digestions, and reaction product and infrared analyses were used to characterize the component GAGs. DEAE-cellulose chromatography was ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(84)90061-3

    authors: Donoff RB,Swann DA,Schweidt SH

    更新日期:1984-02-01 00:00:00

  • Molecular characterization of a novel endonuclease (Xib) and possible involvement in lysosomal glycogen storage disorders.

    abstract::We cloned and partially characterized a human endonuclease (Xib) which shows sequence homologies to pancreatic DNase I but an enzymatic activity closer to DNase II. We report on the structural differences found between Xib and other recently cloned human DNases. Fluores cence microscopy analysis of transiently transfe...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1006/exmp.1999.2254

    authors: Malferrari G,Mazza U,Tresoldi C,Rovida E,Nissim M,Mirabella M,Servidei S,Biunno I

    更新日期:1999-06-01 00:00:00

  • Quantitative assessment of azoxymethane-induced aberrant crypt foci in inbred mice.

    abstract::Heritable differences in tumor susceptibility are observed in mice after repetitive exposures to the organotropic colon carcinogen azoxymethane (AOM). The following study was undertaken to determine whether early morphological alterations within the colonic epithelium correlate with subsequent cancer risk. A/J and SWR...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/s0014-4800(99)80003-3

    authors: Delker DA,Wang QS,Papanikolaou A,Whiteley HE,Rosenberg DW

    更新日期:1999-02-01 00:00:00

  • Distribution of N-methyl-D-aspartate receptors (NMDARs) in the developing rat brain.

    abstract::The N-methyl-D-aspartate receptor (NMDAR), which is one of the glutamate receptors, is considered to have a close relationship to synaptic plasticity in the developing brain. In addition, it is known that the excessive stimulation of NMDARs can trigger neuronal apoptosis. In this study, we examined the distribution of...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/s0014-4800(03)00030-3

    authors: Takai H,Katayama K,Uetsuka K,Nakayama H,Doi K

    更新日期:2003-08-01 00:00:00

  • Keratinocyte growth factor-transfection-stimulated adhesion of colorectal cancer cells to extracellular matrices.

    abstract::The keratinocyte growth factor (KGF) regulates cell growth and behavior in an autocrine or paracrine manner. In colorectal cancer tissues, KGF is expressed in tumor cells and adjacent stromal fibroblasts. We have constructed a KGF-gene-transfected cell line (HCT15-KGF) from a colorectal cancer cell line, HCT-15, that ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2007.07.001

    authors: Kudo M,Ishiwata T,Nakazawa N,Kawahara K,Fujii T,Teduka K,Naito Z

    更新日期:2007-12-01 00:00:00

  • Cytoskeleton-dependent surface blebbing induced by the polar solvent N-methylformamide.

    abstract::In vivo and in vitro studies performed on the polar solvent N-methylformamide (NMF), as well as on its association with chemotherapeutic agents or X rays, have clearly demonstrated that this compound is capable of inducing changes in biological characteristics of tumor cells, e.g., cell differentiation. However, the m...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(92)90001-r

    authors: Malorni W,Meschini S,Arancia G

    更新日期:1992-10-01 00:00:00

  • The effect of bezafibrate and clofibrate on cholesterol ester metabolism in rabbit peritoneal macrophages stimulated with acetylated low density lipoproteins.

    abstract::Bezafibrate and clofibrate reduce the quantity of esterified cholesterol, decrease the incorporation of 14C-labeled oleic acid into cholesterol ester and inhibit acyl coenzyme A cholesterol acyltransferase activity in rabbit peritoneal macrophages stimulated with acetylated low density lipoprotein. In all cases, the e...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(83)90100-4

    authors: Hudson K,Mojumder S,Day AJ

    更新日期:1983-02-01 00:00:00

  • Cytochrome P450 isozyme distribution in normal and tumor-bearing hepatic tissue from rainbow trout (Salmo gairdneri).

    abstract::An immunohistochemical technique was used to localize the major constitutive cytochrome P450 isozyme, P450 LM2, and the major beta-naphthoflavone-inducible isozyme, P450 LM4b, in the livers of untreated and aflatoxin B1 (AFB1)-initiated, tumor-bearing rainbow trout. In hepatic tissue sections from untreated trout, no ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(89)90044-0

    authors: Lorenzana RM,Hedstrom OR,Gallagher JA,Buhler DR

    更新日期:1989-06-01 00:00:00

  • IDH1 mutations in gliomas: first series from a tertiary care centre in India with comprehensive review of literature.

    abstract:OBJECT:Mutations of the gene encoding isocitrate dehydrogenase (IDH) have been shown in a significant proportion of diffuse gliomas. These mutations are specific to gliomas and their utility for diagnosis and prognostication of these tumors is being proclaimed. The present study was conducted with the aim of assessing ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexmp.2011.04.017

    authors: Jha P,Suri V,Sharma V,Singh G,Sharma MC,Pathak P,Chosdol K,Jha P,Suri A,Mahapatra AK,Kale SS,Sarkar C

    更新日期:2011-08-01 00:00:00

  • Phosphorylated SIRT1 as a biomarker of relapse and response to treatment with glatiramer acetate in multiple sclerosis.

    abstract::We have previously shown that SIRT1 mRNA expression was significantly lower in relapsing MS patients compared to those in remission. Our goal was to longitudinally investigate the role of active, phosphorylated SIRT1 (p-SIRT1) as a potential biomarker of relapse and predictor for response to glatiramer acetate (GA) tr...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2018.07.008

    authors: Ciriello J,Tatomir A,Hewes D,Boodhoo D,Anselmo F,Rus V,Rus H

    更新日期:2018-10-01 00:00:00

  • Methylation of tumor associated genes in tissue and plasma samples from liver disease patients.

    abstract::To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of tissue and 42 cases of plasma samples from HCC and liver cirrhos...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2008.07.001

    authors: Chang H,Yi B,Li L,Zhang HY,Sun F,Dong SQ,Cao Y

    更新日期:2008-10-01 00:00:00

  • Effect of oxytocin on lipid accumulation under inflammatory conditions in human macrophages.

    abstract:INTRODUCTION AND AIMS:Oxytocin (OT) is a neuropeptide hormone secreted by the posterior pituitary gland. Deficits in OT action have been observed in patients with behavioral and mood disorders, some of which correlate with an increased risk of cardiovascular disease (CVD). Recent research has revealed a wider systemic ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2021.104604

    authors: Karten A,Vernice NA,Renna HA,Carsons SE,DeLeon J,Pinkhasov A,Gomolin IH,Glass DS,Reiss AB,Kasselman LJ

    更新日期:2021-01-09 00:00:00

  • Sequential change of DNA synthesis in cultured aortic smooth muscle cells stimulated by hyperlipidemic serum.

    abstract::Smooth muscle cells from monkey aorta quiescent in 5% calf serum have been shown to be stimulated to renewed proliferation by hyperlipidemic serum or LDL from such serum. This proliferative response evidently is not dependent on platelet-derived growth factor present in our system in large quantities. The least exposu...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(88)90043-3

    authors: Mitsumata M,Fischer-Dzoga K,Getz GS,Wissler RW

    更新日期:1988-02-01 00:00:00

  • Aortic calcification in chronic fluoride poisoning: biochemical and electronmicroscopic evidence.

    abstract::Fluoride is known to cause ectopic calcification. The biochemical mechanism(s) involved in the initiation of calcification is not understood and the accompanying ultrastructural changes remain to be elucidated. Therefore, certain relevant parameters have been investigated in the aorta of rabbits administered fluoride,...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(90)90025-9

    authors: Susheela AK,Kharb P

    更新日期:1990-08-01 00:00:00

  • Human S-antigen: peptide determinant recognition in uveitis patients.

    abstract::Uveitis is an inflammation of the uveal tract and is one of the major causes of visual impairment. Several lines of evidence suggest an important role for activated T lymphocytes in the perpetuation of posterior uveitis. In sequel to our preliminary observations with human S-antigen, we have further investigated the p...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2003.10.007

    authors: Tripathi P,Saxena S,Yadav VS,Naik S,Singh VK

    更新日期:2004-04-01 00:00:00

  • Contradictory effects of short- and long-term hyperglycemias on ischemic injury of myocardium via intracellular signaling pathway.

    abstract::Although clinical diabetes mellitus is obviously a high risk factor for myocardial infarction, there is disagreement about the sensitivity of ischemic injury of an infarcted myocardium in experimental studies. The present study evaluated the influences of different durations of hyperglycemia on ischemic and reperfusio...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2003.08.003

    authors: Xu G,Takashi E,Kudo M,Ishiwata T,Naito Z

    更新日期:2004-02-01 00:00:00

  • A time course study demonstrating RNA stability in postmortem skin.

    abstract::Knowledge of the factors regulating the rate of mRNA degradation, including postmortem delay, is important in determining the reliability of gene expression patterns in dermal tissue. Since RNA stability can be tissue dependent, this study evaluates the effect of postmortem interval on the integrity of total RNA or th...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2006.11.001

    authors: Gopee NV,Howard PC

    更新日期:2007-08-01 00:00:00

  • Hemodynamic and electrophysiological effects of mercury in intact anesthetized rabbits and in isolated perfused hearts.

    abstract::Using intact anesthetized rabbits and isolated perfused hearts, the hemodynamic and electrophysiological effects of mercury (Hg) were examined in order to assess the role of cardiovascular dysfunction in Hg intoxication. The most consistent and prominent cardiovascular effect was a significant reduction in blood press...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(89)90038-5

    authors: Rhee HM,Choi BH

    更新日期:1989-06-01 00:00:00

  • Histopathological effects of homocysteine thiolactone on epithelial and stromal tissues.

    abstract::To study the effects of homocysteine on epithelial and stromal tissues, the free base of homocysteine thiolactone was synthesized and administered to mice. Intraperitoneal and intramuscular doses of 0.2 to 2.0 mg/g are acutely toxic, causing as much as 90% mortality because of intense tissue necrosis at the injection ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(89)90016-6

    authors: McCully KS,Vezeridis MP

    更新日期:1989-10-01 00:00:00

  • Differential modulation of gene expression among rat tissues with warm ischemia.

    abstract::The aim of this study is to determine if warm ischemia after surgical extirpation impacts gene expression in tissue samples which will be used for cDNA array analysis. We investigated effects of warm ischemia on gene expression in lung, liver, kidney, and spleen of rats, chronologically, using an original cDNA array, ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2004.07.004

    authors: Miyatake Y,Ikeda H,Michimata R,Koizumi S,Ishizu A,Nishimura N,Yoshiki T

    更新日期:2004-12-01 00:00:00

  • The role of TRB3 in mast cells sensitized with monomeric IgE.

    abstract::Mast cells play a key role in immunoglobulin E (IgE)-associated allergic disorders; however, the cellular effects of sensitization remain poorly understood. Using gene microarrays and the multiplexing ELISA method, we examined the effects of sensitization on RBL-CCR1 cell transcription and chemokine/cytokine secretion...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2012.09.008

    authors: Kuo CH,Morohoshi K,Aye CC,Garoon RB,Collins A,Ono SJ

    更新日期:2012-12-01 00:00:00

  • Induction of antigen-specific human T suppressor cells by membrane and soluble ILT3.

    abstract::Antigen-specific CD8 suppressor T cells (CD8(+) Ts) are adaptive regulatory T cells that are induced in vivo and in vitro by chronic antigenic stimulation of human T cells. CD8(+) Ts induce the upregulation of the inhibitory receptors ILT3 and ILT4 on monocytes and dendritic cells rendering these antigen presenting ce...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2012.09.011

    authors: Vlad G,Suciu-Foca N

    更新日期:2012-12-01 00:00:00

  • SFRP1 promotor methylation analysis of FTA card touch-prep samples derived from colonic polyps.

    abstract::Whatman FTA® cards provide the most reliable method for DNA storage and extraction, however, the literature lacks reports on the epigenetic analysis of FTA card-derived tumor DNA. Therefore, this study aimed at demonstrating that punches from colonic adenoma samples preserved on FTA filter cards are suitable for methy...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2020.104397

    authors: Buxhofer-Ausch V,Ausch C,Reiner A,Müllner-Ammer K,Schmid A,Kriwanek S,Sebesta C,Halwachs-Baumann G,Kriegshäuser G

    更新日期:2020-06-01 00:00:00

  • Matrix metalloproteinases in acute inflammation: induction of MMP-3 and MMP-9 in fibroblasts and epithelial cells following exposure to pro-inflammatory mediators in vitro.

    abstract::Recent studies have demonstrated important pro-inflammatory roles for two matrix metalloproteinases (MMPs)-MMP-3 (stromelysin-1) and MMP-9 (gelatinase B)-in acute lung injury [Am. J. Respir. Cell Mol. Biol. 24 (2001) 1]. A role for MMP-3 in skin inflammation has also been demonstrated [Proc. Natl. Acad. Sci. U. S. A. ...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2004.01.003

    authors: Warner RL,Bhagavathula N,Nerusu KC,Lateef H,Younkin E,Johnson KJ,Varani J

    更新日期:2004-06-01 00:00:00

  • Antiandrogen abiraterone and docetaxel treatments affect Notch1, Jagged1 and Hes1 expressions in metastatic prostate cancer cells.

    abstract:BACKGROUND:Prostate cancer is the most common cancer in men. A Notch signaling pathway is an important pathway in cell proliferation, differentiation, and fate. However, currently, the effects of abiraterone based-anti-androgene therapy and docetaxel, the most commonly used standard chemotherapy in prostate cancer trea...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2021.104607

    authors: Soylu H,Kırca M,Avcı S,Ozpolat B,Ustunel I

    更新日期:2021-01-19 00:00:00

  • The Apolipoprotein B/Apolipoprotein A 1 ratio in relation to metabolic syndrome and its components in a sample of the Tunisian population.

    abstract:OBJECTIVE:This study was undertaken to investigate the relationship between the Apolipoprotein B/Apolipoprotein A 1 (ApoB/ApoA 1) ratio and various characteristics of the metabolic syndrome (MetS) in a sample of the Tunisian population. METHODS:The study included 330 adults aged 35-74 (172 patients with MetS and 158 c...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2011.06.007

    authors: Belfki H,Ben Ali S,Bougatef S,Ben Ahmed D,Haddad N,Jmal A,Abdennebi M,Ben Romdhane H

    更新日期:2011-10-01 00:00:00

  • Transcription factors associated with epithelial-mesenchymal transition and cancer stem cells in the tumor centre and margin of invasive breast cancer.

    abstract::Although tumor surgery aims for a complete resection respecting tumor-specific safety distance, in many cases the most peripheral part, the invasion front, remains in situ. Tumor cells at the tumor margin lose epithelial properties and acquire features of mesenchymal cells. The process of epithelial-to-mesenchymal tra...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2012.09.003

    authors: Alkatout I,Wiedermann M,Bauer M,Wenners A,Jonat W,Klapper W

    更新日期:2013-02-01 00:00:00

  • Effect of acute plasma fibronectin depletion on tissue fibronectin levels: analysis by a new fluorescent immunoassay.

    abstract::Plasma fibronectin modulates reticuloendothelial (RE) phagocytosis of cellular and tissue debris, fibrin microaggregates, and gelatin-coated particulates. An antigenically related, but more insoluble form of fibronectin is found in various tissues and suspected to play a role in vascular permeability, cell adhesion, a...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/0014-4800(84)90010-8

    authors: Saba TM,Cho E,Blumenstock FA

    更新日期:1984-08-01 00:00:00

  • Tranilast treatment decreases cell growth, migration and inhibits colony formation of human breast cancer cells.

    abstract::In the treatment of breast cancer, although a wide of choice of drugs and treatment modalities are available, drug resistance or drug toxicity poses a considerable challenge. Tranilast is a well tolerated drug used in the treatment of allergic disorders. Previous works in various models have shown that tranilast has t...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2010.10.012

    authors: Subramaniam V,Ace O,Prud'homme GJ,Jothy S

    更新日期:2011-02-01 00:00:00

  • In situ detection of SOCS and cytokine expression in the uterine cervix from HIV/HPV coinfected women.

    abstract::The purpose of this study was to look for associations between a newly described class of suppressors of cytokine signaling (SSI/SOCS) and cytokine expression in the uterine cervix from HIV/HPV coinfected women. We examined the pro-inflammatory cytokines TNF-alpha and IL-6 since their expressions are linked and respon...

    journal_title:Experimental and molecular pathology

    pub_type: 杂志文章

    doi:10.1016/j.yexmp.2006.01.002

    authors: Nicol AF,Nuovo GJ,Wang Y,Grinsztejn B,Tristão A,Russomano F,Perez MA,Lapa e Silva JR,Fernandes AT,Gage JR,Martínez-Maza O,Bonecini-Almeida MG

    更新日期:2006-08-01 00:00:00