Abstract：
:Recent insights have implicated mesothelial-to-mesenchymal transition (MMT) as a mechanism by which mesothelial cells can transdifferentiate into cancer-associated fibroblasts (CAFs) in several cancers metastasizing to the peritoneum. However, this was not evaluated extensively in colorectal cancer. We examined the presumed mesothelial origin of CAFs in three types of colorectal carcinoma: conventional type adenocarcinoma, mucinous carcinoma and signet ring cell carcinoma. We evaluated the expression of mesothelial, mesenchymal, angiogenesis and colorectal cancer-related markers in peritoneal samples of twelve colorectal cancer patients with peritoneal carcinomatosis and four control patients by immunohistochemistry. We observed morphological and immunohistochemical changes in the vicinity of tumor implants in all studied colorectal cancer types. Mesothelial cells acquired a spindle-shaped myofibroblast-like morphology, lost expression of mesothelial markers, and gained expression of mesenchymal markers. Analysis of consecutive tissue sections and double staining for mesothelial and mesenchymal markers revealed overlap in expression of mesothelial and CAF markers. These findings are highly suggestive of a mesothelial origin of CAFs in peritoneal carcinomatosis in colorectal cancer. Interfering with the process of MMT might be a valuable approach in treating and preventing peritoneal carcinomatosis. Differences observed between colorectal cancer types suggest that one single strategy might not be applicable.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Demuytere J,Ceelen W,Van Dorpe J,Hoorens Adoi
10.1016/j.yexmp.2020.104442subject
Has Abstractpub_date
2020-08-01 00:00:00pages
104442eissn
0014-4800issn
1096-0945pii
S0014-4800(20)30100-3journal_volume
115pub_type
杂志文章abstract：:Selected estimates of lipid peroxidation were analyzed in mouse quadriceps femoris muscle immediately after submaximal prolonged (9 hr) and exhaustive maximal running (2-3 hr), and at intervals 1-10 days afterward during the exercise-induced myopathy. Immediately after the two types of exertion no significant changes ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
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pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
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journal_title：Experimental and molecular pathology
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更新日期：2016-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2018.11.005
更新日期：2019-02-01 00:00:00
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pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.11.005
更新日期：2016-12-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2007.04.007
更新日期：2007-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2007.07.001
更新日期：2007-12-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.04.004
更新日期：2005-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：1995-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1026
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.12.002
更新日期：2010-04-01 00:00:00
