Abstract：
:Invadopodia, cancer cell protrusions with proteolytic activity, are functionally associated with active remodeling of the extracellular matrix. Here, we show that the invadopodia-related protein TKS5 is expressed in human pancreatic adenocarcinoma lines, and demonstrate that pancreatic cancer cells depend on TKS5 for invadopodia formation and function. Immunofluorescence staining of human pancreatic cancer cells reveals that TKS5 is a marker of mature and immature invadopodia. We also analyze the co-staining patterns of TKS5 and the commonly used invadopodia marker Cortactin, and find only partial co-localization of these two proteins at invadopodia, with a large fraction of TKS5-positive invadopodia lacking detectable levels of Cortactin. Whereas compelling evidence exist on the role of invadopodia as mediators of invasive migration in cultured cells and in animal models of cancer, these structures have never been detected inside human tumors. Here, using antibodies against TKS5 and Cortactin, we describe for the first time structures strongly resembling invadopodia in various paraffin-embedded human tumor surgical specimens from pancreas and other organs. Our results strongly suggest that invadopodia are present inside human tumors, and warrants further investigation on their regulation and occurrence in surgical specimens, and on the value of TKS5 antibodies as pathological research and diagnostic tools.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Chen YC,Baik M,Byers JT,Chen KT,French SW,Díaz Bdoi
10.1016/j.yexmp.2018.11.005subject
Has Abstractpub_date
2019-02-01 00:00:00pages
17-26eissn
0014-4800issn
1096-0945pii
S0014-4800(18)30487-8journal_volume
106pub_type
杂志文章abstract：:An immunohistochemical technique was used to localize the major constitutive cytochrome P450 isozyme, P450 LM2, and the major beta-naphthoflavone-inducible isozyme, P450 LM4b, in the livers of untreated and aflatoxin B1 (AFB1)-initiated, tumor-bearing rainbow trout. In hepatic tissue sections from untreated trout, no ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(89)90044-0
更新日期：1989-06-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1995.1005
更新日期：1995-02-01 00:00:00
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journal_title：Experimental and molecular pathology
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doi：10.1016/j.yexmp.2020.104442
更新日期：2020-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(87)90037-2
更新日期：1987-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90062-0
更新日期：1985-08-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2016.05.015
更新日期：2016-12-01 00:00:00
abstract：:Integrins are a family of cell surface glycoproteins that act as receptors for ECM proteins or for membrane bound counter-receptors on other cells. The integrin receptor family of vertebrates includes at least 16 distinct alpha subunits and at least 8 beta subunits which can associate to form more than 20 distinct int...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.04.004
更新日期：2005-08-01 00:00:00
abstract：:Unilateral torsion of the spermatic cord was surgically induced in 24 Hartley strain guinea pigs. Groups of six animals were sacrificed 4, 8, 12, and 16 months after the surgery and testes were excised. Nine animals has severely damaged testes. Extensive study on the Sertoli cells of each of these nine animals was car...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(86)90072-9
更新日期：1986-04-01 00:00:00
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journal_title：Experimental and molecular pathology
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doi：10.1016/j.yexmp.2012.09.008
更新日期：2012-12-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.06.020
更新日期：2015-10-01 00:00:00
abstract：BACKGROUND:Existing clinical data have shown that high-grade prostatic intraepithelial neoplasia (HGPIN) is the most likely precursor to prostate cancer (CaP). Criteria to distinguish HGPIN that progress to CaP from those that do not remain poorly defined. Our objective was to evaluate microvessel density as a molecula...
journal_title：Experimental and molecular pathology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.yexmp.2004.04.005
更新日期：2004-10-01 00:00:00
abstract：:Viral pathogens have been implicated in the development of certain cancers including human papillomavirus (HPV) in squamous cell carcinoma and Epstein-Barr virus (EBV) in Burkitt's lymphoma. The significance of viral pathogens in brain tumors is controversial, and human cytomegalovirus (HCMV) has been associated with ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.03.010
更新日期：2014-06-01 00:00:00
abstract：:Increased levels of prolactin (PRL) have recently been associated with carcinogenesis and the exacerbation of autoimmune diseases, and might be involved in the progression of tuberculosis (TB). To investigate the relationship between PRL and prolactin receptor (PRLr) expression with inflammatory response and apoptosis...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2015.03.016
更新日期：2015-06-01 00:00:00
abstract：:Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer next to hepatocellular carcinoma (HCC). Despite the significant difference of the therapeutic strategy for both diseases, their histological appearance may be very similar. Thus the correct diagnosis is crucial for treatment choice bu...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.04.002
更新日期：2014-06-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1993.1004
更新日期：1993-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1994.1037
更新日期：1994-12-01 00:00:00
abstract：:Chronic heart failure (CHF) is a common disease in clinical practice, and its incidence has been increasing in recent years. Understanding the pathogenesis of CHF is the key to its future clinical diagnosis and treatment. Molecular research is a hot topic in modern hospitals, and long non-coding RNA (LncRNA) has been ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2020.104529
更新日期：2020-12-01 00:00:00
abstract：:Sulfur mustard (SM) is a bifunctional alkylating agent causing skin inflammation, edema and blistering. A hallmark of SM-induced toxicity is follicular and interfollicular epithelial damage. In the present studies we determined if SM-induced structural alterations in hair follicles and sebaceous glands were correlated...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.03.002
更新日期：2014-06-01 00:00:00
abstract：:Esophageal squamous cell carcinoma (ESCC) is highly fatal due to late diagnosis and inefficient treatment. Early disease detection could improve diagnosis and patient survival. Esophageal squamous epithelial cells express SPRR3, a member of the small proline-rich protein family, which is downregulated in ESCC. Therefo...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2011.06.006
更新日期：2011-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2009.07.006
更新日期：2009-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/s0014-4800(03)80006-0
更新日期：2003-02-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.yexmp.2013.08.005
更新日期：2013-12-01 00:00:00
abstract：:Antigen-specific CD8 suppressor T cells (CD8(+) Ts) are adaptive regulatory T cells that are induced in vivo and in vitro by chronic antigenic stimulation of human T cells. CD8(+) Ts induce the upregulation of the inhibitory receptors ILT3 and ILT4 on monocytes and dendritic cells rendering these antigen presenting ce...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2012.09.011
更新日期：2012-12-01 00:00:00
abstract：:In vivo and in vitro studies performed on the polar solvent N-methylformamide (NMF), as well as on its association with chemotherapeutic agents or X rays, have clearly demonstrated that this compound is capable of inducing changes in biological characteristics of tumor cells, e.g., cell differentiation. However, the m...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(92)90001-r
更新日期：1992-10-01 00:00:00
abstract：BACKGROUND:Calcific tendinopathy (CT) is characterized by deposits of calcium, most commonly found in the shoulder tendons. The exact cause and pathogenesis of CT are not fully understood. This study analyzed the expression pattern of RNA-binding protein fox-1 homolog 2 (RBFOX2), a crucial splicing regulator in tissue ...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2019.104264
更新日期：2019-08-01 00:00:00
abstract：:A primary culture of cells derived from uninvolved and atherosclerotic intima of human aorta was used to elucidate the role of cyclic nucleotides in atherogenesis. The cells cultured from fatty streaks and atherosclerotic plaques had a 2- to 8-fold lower cyclic AMP level and a 1.5- to 2-fold higher level of cyclic GMP...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(87)90020-7
更新日期：1987-12-01 00:00:00
abstract：:The alterations, visible by light and electron microscopy, in the distal airway cells of Syrian golden hamsters were analyzed at several time intervals, from 6 hr to 4 weeks, after a single subcutaneous injection (120 mg/kg body wt) of 4-nitroquinoline 1-oxide (4NQO), a potent carcinogen. 4NQO induced injurious effect...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(85)90029-2
更新日期：1985-04-01 00:00:00
abstract：:Ischemia has elicited a great deal of interest among the scientific community due to its role in life-threatening pathologies such as cancer, stroke, acute renal failure, and myocardial infarction. Oxygen deprivation (hypoxia) associated with ischemia has recently become a subject of intense scrutiny. New investigator...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2007.03.002
更新日期：2007-10-01 00:00:00
abstract：:Since the first mitochondrial dysfunction was described in the 1960s, the medicine has advanced in its understanding the role mitochondria play in health, disease, and aging. A wide range of seemingly unrelated disorders, such as schizophrenia, bipolar disease, dementia, Alzheimer's disease, epilepsy, migraine headach...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章,评审
doi：10.1016/j.yexmp.2006.09.008
更新日期：2007-08-01 00:00:00
abstract：:The hamster cheek pouch is an excellent target tissue for the experimental study of oral carcinogenesis. In the course of searching for molecular alterations during the malignant transformation process, the necessity for a molecular marker for cellular proliferation became apparent. In this report, we show that the ce...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/0014-4800(88)90034-2
更新日期：1988-10-01 00:00:00
