Abstract：
:Ketamine, a common N-methyl-d-aspartate receptor (NMDAR) antagonist, is an option for cancer pain treatment in clinical practice. Ketamine has been shown to have the capacity to attenuate cancer cells malignancy. However, the underlying mechanism remains elusive. In the present study, we reported that ketamine inhibited the malignant potential of colorectal cancer cells and investigated the possible mechanisms involved. Ketamine suppressed the expression of VEGF, HIF-1α, p-AKT, p-ERK, and p-CaMK II, and reduced intracellular Ca2+ level in a concentration dependent manner (1, 5, 10 μg/ml). Furthermore, AP5 and MK801 (NMDAR inhibitors), and KN93 (CaMK II inhibitor), decreased the expression of VEGF, HIF-1a, p-AKT, p-ERK, and p-CaMK II, which were similar to the effect of ketamine. Further, the anti-tumor effect of ketamine was reversed by d-serine (NMDAR activator). Ketamine did not affect NMDA receptor expression, however knockdown of NMDA receptor using siRNA attenuated the effect of ketamine on cell migration. Collectively, these findings demonstrated that ketamine attenuated the expression of VEGF and cell migration ability in colorectal cancer cells, probably via blockage of NMDA receptor.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Duan W,Hu J,Liu Ydoi
10.1016/j.yexmp.2019.02.004subject
Has Abstractpub_date
2019-04-01 00:00:00pages
171-178eissn
0014-4800issn
1096-0945pii
S0014-4800(18)30498-2journal_volume
107pub_type
杂志文章abstract：:Two basic models of alcoholic liver disease pathogenesis exist, one in vivo and one in vitro. To justify the in vitro model, evidence is needed to show that it stimulates the in vivo model. Therefore, changes in gene expression caused by high ethanol level were compared using the two models. Many functional pathways w...
journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
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doi：10.1016/j.yexmp.2011.01.002
更新日期：2011-06-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.01.010
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1006/exmp.1994.1037
更新日期：1994-12-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2014.03.002
更新日期：2014-06-01 00:00:00
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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journal_title：Experimental and molecular pathology
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更新日期：1985-04-01 00:00:00
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journal_title：Experimental and molecular pathology
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更新日期：2018-12-01 00:00:00
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journal_title：Experimental and molecular pathology
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更新日期：2011-10-01 00:00:00
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journal_title：Experimental and molecular pathology
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更新日期：1984-12-01 00:00:00
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journal_title：Experimental and molecular pathology
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更新日期：1993-12-01 00:00:00
abstract：BACKGROUND:Somatic mutation of the BRAF gene is one to be the most commonly known genetic change in thyroid tumors especially papillary thyroid cancers. The T1799A activating point mutation is detected in >98% of the thyroid tumors, and result in substitution of amino acid valine at position 600 to glutamic acid. METH...
journal_title：Experimental and molecular pathology
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更新日期：2018-12-01 00:00:00
abstract：:Cell death caused by the accumulation of extracellular adenosine is believed to contribute to the profound loss of T lymphocytes in patients with severe combined immunodeficiency disease due to adenosine deaminase deficiency. Although adenosine is known to trigger apoptosis in thymocytes and peripheral T cells, the mo...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2005.08.001
更新日期：2005-12-01 00:00:00
abstract：:Previously, we described an orthotopic cholangiocarcinoma model based on bile duct inoculation of spontaneously-transformed low grade malignant rat BDE1 cholangiocytes (BDEsp cells) compared to high grade malignant erbB-2/neu- transformed BDE1 cholangiocytes (BDEneu cells) into the livers of syngeneic rats, which clos...
journal_title：Experimental and molecular pathology
pub_type： 杂志文章
doi：10.1016/j.yexmp.2010.08.007
更新日期：2010-12-01 00:00:00
