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Cytoskeletal changes induced in vitro by 2,5-hexanedione: an immunocytochemical study.

Abstract：

:The effects of 2,5-hexanedione, the main metabolite of the solvents hexane and methyl butyl ketone, have been explored in different in vitro epithelial (CG5 and HEp-2) and melanoma (JR8) cells by means of immunochemistry and electron microscopy. The administration of the toxicant to the cell monolayers at noncytolytic concentrations for 24 and 48 hr exerted several effects on the cell lines studied. Most epithelial and melanoma cells detached from the substrate were in the mitotic phase, whereas cells adhering to the substrate showed time-dependent organelle changes. In fact, after treatment with 2,5-hexanedione, mitochondria appeared swollen, with distorted cristae and rarefied matrix; changes in intracytoplasmic vesicles were also detected. Cytoskeletal components were also investigated. A remarkable rearrangement of microfilaments and intermediate filaments (keratin and vimentin) was detected in a time-dependent manner. In particular, actin ruffles and intermediate filament aggregates were observed. Furthermore, the microtubular apparatus seemed to be less affected. The results here reported seem to indicate cytoskeletal components as probable targets of 2,5-hexanedione cytotoxicity in cultured cells.

journal_name

Exp Mol Pathol

journal_title

Experimental and molecular pathology

authors

Malorni W,Iosi F,Formisano G,Arancia G

doi

10.1016/0014-4800(89)90056-7

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

50-68

issue

1

eissn

0014-4800

issn

1096-0945

pii

0014-4800(89)90056-7

journal_volume

50

pub_type

杂志文章

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