Abstract：
:The proteasome interacts with a large number of proteins which regulate specific cellular functions. The focus of this study is to examine the proteasome interaction with Delta-aminolevulinate dehydratase (ALAD). ALAD is involved in the heme biosynthesis pathway and was co-isolated, with the 20S proteasome using several chromatographic purification steps. The MALDI-TOF mass spectrometry analysis identified this proteasome co-isolated protein as ALAD. When the proteasome was isolated using density-gradient centrifugation, ALAD was also found in the 26S proteasome fractions. It co-isolated with the 20S more than with the 26S proteasome. Furthermore, immunoprecipitated ALAD stained positive with antibodies to proteasome subunits. These results indicate that ALAD might interact with the proteasome. It is possible that ALAD is involved in modulating proteasome activity. When purified proteasomes were incubated with ALAD it was found that ALAD changes proteasome activity in a dose dependent manner. This indicates that ALAD may play a significant role in regulating proteasome activity. The data supports the hypothesis that ALAD, an important enzyme for heme synthesis, is also important as a proteasome interacting protein.
journal_name
Exp Mol Patholjournal_title
Experimental and molecular pathologyauthors
Bardag-Gorce F,French SWdoi
10.1016/j.yexmp.2011.05.003subject
Has Abstractpub_date
2011-10-01 00:00:00pages
485-9issue
2eissn
0014-4800issn
1096-0945pii
S0014-4800(11)00061-Xjournal_volume
91pub_type
杂志文章abstract：:The effect of hypertension, hyperlipidemia, and the combination of both on acute and chronic myocardial ischemia were evaluated in a total of 30 male rabbits. After preliminary hypertension and/or hyperlipidemic load by loading of the abdominal aorta and/or cholesterol feeding, acute ischemia was produced by clipping ...
journal_title：Experimental and molecular pathology
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更新日期：2018-12-01 00:00:00
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更新日期：2000-10-01 00:00:00
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更新日期：2014-06-01 00:00:00
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pub_type： 杂志文章,多中心研究
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1991-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
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journal_title：Experimental and molecular pathology
pub_type： 杂志文章
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更新日期：2010-06-01 00:00:00
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