当前位置： SCI文献检索 > EXPERIMENTAL AND MOLECULAR PATHOLOGY期刊下所有文献 > Concordance of DNA methylation pattern in plasma and tumor DNA of Egyptian hepatocellular carcinoma patients.

Concordance of DNA methylation pattern in plasma and tumor DNA of Egyptian hepatocellular carcinoma patients.

Abstract：

:Hepatocellular carcinoma (HCC) is the fifth most common cancer and the fourth leading cause of cancer mortality globally. HCC incidence has doubled in Egypt in the past 10 years, which could be attributed to the high prevalence of hepatitis C virus (HCV) and hepatitis B virus (HBV), although HBV rates have declined after the introduction of the vaccine in 1992. Aberrant DNA methylation may play an important role in hepatocarcinogenesis. Liver biopsy is the current gold standard for methylation studies; however, imaging techniques often suffice for diagnosis making tissue samples increasingly scarce. The efficacy of conducting DNA methylation studies in molecular epidemiology using plasma DNA is still unclear. We compared tumor methylation profile for the tumor suppressor genes APC, FHIT, p15, p16, and E-cadherin in tumor tissues and plasma to test the concordance between the two types of specimen from the same HCC patients. Twenty-eight HCC patients with matching tissue and plasma DNA were recruited from a case-control study in Gharbiah, Egypt. Concordance between the tissue and plasma was statistically significant in all five genes as follows: APC (23/28, 82.1%, p=0.001), FHIT (24/28, 85.7%, p=.0001), p15 (25/28, 89.2%, p=0.045), p16 (19/28, 67.9%, p=0.037), and E-cadherin (22/28, 78.5%, p=0.0008). The average specificity was 90%, 86%, 96%, 86%, and 100%, respectively. There was no significant association between methylation and hepatitis viral infection for any of the genes tested in this study. Plasma DNA can be reliable for testing methylation profile in liver cancer patients in this population. Future studies on a larger sample size should investigate methylation profile in populations with higher rates of HBV, HCV, and other risk factors.

journal_name

Exp Mol Pathol

journal_title

Experimental and molecular pathology

authors

Iyer P,Zekri AR,Hung CW,Schiefelbein E,Ismail K,Hablas A,Seifeldin IA,Soliman AS

doi

10.1016/j.yexmp.2009.09.012

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

107-11

issue

1

eissn

0014-4800

issn

1096-0945

pii

S0014-4800(09)00125-7

journal_volume

88

pub_type

杂志文章

相关文献

EXPERIMENTAL AND MOLECULAR PATHOLOGY文献大全
  • Characterization of the anti-inflammation mechanism for the AO herbal extract.

    abstract：:A broad range of cytokines are secreted during the inflammatory response by the immune system. Some cytokines promote inflammation, while others inhibit inflammation. Inflammatory cytokines work in harmony when they encounter external pathogens or internal dangers. Inflammation is resolved after the cause is eliminate...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2016.11.005

    authors： Ye H,Wang Y,Yan J,Jenson AB

    更新日期：2016-12-01 00:00:00

  • Central nervous system (CNS) tumor cell heterogeneity contributes to differential platinum-based response in an in vitro 2D and 3D cell culture approach.

    abstract：:One of the models that best explains the cellular heterogeneity observed in central nervous system (CNS) tumors is the presence of cancer stem cells (CSCs). CSCs can originate from differentiated adult cells that return to an undifferentiated stage through the mechanism known as epithelial-mesenchymal transition (EMT)...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2020.104520

    authors： Gonçalves BÔP,Fialho SL,Silvestrini BR,Sena IFG,Dos Santos GSP,Assis Gomes D,Silva LM

    更新日期：2020-10-01 00:00:00

  • The role of β-catenin and paired-like homeobox 2B (PHOX2B) expression in neuroblastoma patients; predictive and prognostic value.

    abstract：BACKGROUND:The expression of β-catenin and paired-like homeobox 2B (PHOX2B) expression were assessed in Neuroblastoma (NB) patients as a diagnostic, prognostic and/or predictive markers. METHODS:Bone marrow (BM) samples of 52 NB patients were assessed for the expression of β-catenin by immunohistochemistry (IHC), and ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2019.104272

    authors： El-Shazly SS,Hassan NM,Abdellateif MS,El Taweel MA,Abd-Elwahab N,Ebeid EN

    更新日期：2019-10-01 00:00:00

  • Early atherogenesis in White Carneau pigeons: effect of a short-term regression diet.

    abstract：:The intracellular distribution of lipid shifts from cytoplasmic to lysosomal localization during the progression of atherosclerosis. It has been suggested that this shift may relate to regressability of lesions. The effects of reducing plasma cholesterol on the regression of early cholesterol-induced atherosclerosis w...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(90)90046-g

    authors： Jerome WG,Lewis JC

    更新日期：1990-12-01 00:00:00

  • Active systemic lupus erythematosus is associated with decreased blood conventional dendritic cells.

    abstract：OBJECTIVE:The aim of the study is to determine the frequency and functionality of blood conventional dendritic cells (cDCs) in relation to disease activity in systemic lupus erythematosus. METHODS:Blood cDCs were enumerated for 34 SLE patients, defined as "active" (SLEDAI ≥ 4) or "inactive" (SLEDAI < 4), 26 RA subject...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2013.06.003

    authors： Khan SA,Nowatzky J,Jiménez-Branda S,Greenberg JD,Clancy R,Buyon J,Bhardwaj N

    更新日期：2013-10-01 00:00:00

  • TNF signaling in vascular endothelial cells.

    abstract：:Vascular endothelium is a major target of actions of the proinflammatory cytokine tumor necrosis factor (TNF). Increasingly, the intracellular pathways that are activated in response to TNF have been elucidated. Many of these pathways have proven to be cell type-specific, requiring that observations made in other cell...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章,评审

    doi：10.1006/exmp.2001.2368

    authors： Madge LA,Pober JS

    更新日期：2001-06-01 00:00:00

  • Alternative splicing induces cytoplasmic localization of RBFOX2 protein in calcific tendinopathy.

    abstract：BACKGROUND:Calcific tendinopathy (CT) is characterized by deposits of calcium, most commonly found in the shoulder tendons. The exact cause and pathogenesis of CT are not fully understood. This study analyzed the expression pattern of RNA-binding protein fox-1 homolog 2 (RBFOX2), a crucial splicing regulator in tissue ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2019.104264

    authors： Cho N,Kim JO,Lee S,Choi S,Kim J,Ko MS,Park SJ,Ji JH,Kim KK

    更新日期：2019-08-01 00:00:00

  • Effects of 5-azacytidine in Syrian golden hamsters: toxicity, tumorigenicity, and differential modulation of bronchial carcinogenesis.

    abstract：:5-Azacytidine (AZC) was studied in a lung cancer model in outbred and syngeneic (F1D) hamsters wherein benzol[a]pyrene (BP) from sustained release implants (SRI) induces preneoplastic mucosal changes which progress to bronchogenic cancer. In pilot studies to evaluate AZC toxicity, a dose schedule of 5 mg/kg biweekly w...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(90)90022-6

    authors： Hammond WG,Yellin A,Gabriel A,Paladugu RR,Azumi N,Hill LR,Benfield JR

    更新日期：1990-08-01 00:00:00

  • Delta-aminolevulinic dehydratase is a proteasome interacting protein.

    abstract：:The proteasome interacts with a large number of proteins which regulate specific cellular functions. The focus of this study is to examine the proteasome interaction with Delta-aminolevulinate dehydratase (ALAD). ALAD is involved in the heme biosynthesis pathway and was co-isolated, with the 20S proteasome using sever...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2011.05.003

    authors： Bardag-Gorce F,French SW

    更新日期：2011-10-01 00:00:00

  • Localization of fibrinogen during aggregation of avian thrombocytes.

    abstract：:The thrombocyte is the avian equivalent of the mammalian blood platelet and is involved in hemostasis through a fibrinogen-mediated process. Although fibrinogen has been implicated as a molecular bridge between activated cells during aggregation, the location of this molecule and its receptor on thrombocytes has not b...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1994.1035

    authors： O'Toole ET,Hantgan RR,Lewis JC

    更新日期：1994-12-01 00:00:00

  • miR-126 in human cancers: clinical roles and current perspectives.

    abstract：:miR-126 has been implicated in the processes of inflammation and angiogenesis. Through these processes, miR-126 is implicated in cancer biology, but its role there has not been well reviewed. The aim of this review is to examine the molecular mechanisms and clinicopathological significance of miR-126 in human cancers....

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexmp.2013.12.004

    authors： Ebrahimi F,Gopalan V,Smith RA,Lam AK

    更新日期：2014-02-01 00:00:00

  • Evaluation of formalin-free tissue fixation for RNA and microRNA studies.

    abstract：:FineFix, RCL-2 and HOPE, three formalin-free fixatives, were compared to the common used formalin fixed tissue samples of lung cancer and were evaluated for their effects on quality, quantity and integrity of RNA and microRNA. Two commercially available RNA extraction Kits (RNeasy FFPE by Qiagen and RecoverAll™ Nuclei...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2011.05.007

    authors： Arzt L,Kothmaier H,Quehenberger F,Halbwedl I,Wagner K,Maierhofer T,Popper HH

    更新日期：2011-10-01 00:00:00

  • LncRNA KCNQ1OT1 contributes to cardiomyocyte apoptosis by targeting FUS in heart failure.

    abstract：:Long noncoding RNAs (lncRNAs) have recently been recognized as the important regulators in cardiac diseases. This study was aimed to investigate the role and molecular mechanism of lncRNA KCNQ1OT1 in regulating cardiomyocyte apoptosis in heart failure (HF). The mouse model of HF was induced by doxorubicin (ADR). Cell ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2020.104480

    authors： Lai L,Xu Y,Kang L,Yang J,Zhu G

    更新日期：2020-08-01 00:00:00

  • Direct inhibitory effect of uremic toxins and polyamines on proliferation of VERO culture cells.

    abstract：:The dialysate fluid of uremic patients exhibits, in vitro, an inhibitory effect on cell growth, owing to urea, guanidino compounds, and substances named middle molecules. The polyamines are compounds which exhibit high levels in biological fluids during either normal development or disease such as psoriasis, uremia, a...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.1997.2215

    authors： Stabellini G,Mariani G,Pezzetti F,Calastrini C

    更新日期：1997-01-01 00:00:00

  • Tyrphostin AG-556 reduces myocardial infarct size and improves cardiac performance in the rat.

    abstract：:TNF-alpha is a proinflammatory cytokine, abundantly expressed after myocardial infarction. It has been suggested that it exhibits myocardial suppressive and cytotoxic effects. AG-556 is a tyrosine kinase inhibitor synthesized based on its ability to reduce TNF-alpha production and cell toxicity, and to improve experim...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/s0014-4800(03)00022-4

    authors： George J,Biner S,Keren P,Barshack I,Goldberg I,Sherez J,Levitzki A,Keren G,Roth A

    更新日期：2003-06-01 00:00:00

  • Selected pulmonary biochemical and hematological changes produced by prolonged hypoxia in the rat.

    abstract：:Rats were exposed to 12% O2 (1 atm) for 48 hr, then 10% O2 for the duration of the exposures. Significant elevations in enzymes of the glutathione peroxidase system were found in the lungs of rats killed 3.5, 7.5, and 12.5 days of exposure compared to air-breathing controls. Superoxide dismutase was also elevated afte...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(86)90022-5

    authors： Reddy AK,Kimball RE,Omaye ST

    更新日期：1986-12-01 00:00:00

  • Postinflammatory increase of absorption from peritoneal cavity into lymph nodes: particulate and oily inocula.

    abstract：:One week after intraperitoneal injection of rats and mice with a chemical irritant, the abdominal cavity was in the healing phase of a sterile peritonitis. At this time, mineral oil or a particulate dye or metal injected intraperitoneally was absorbed into lymph nodes in much larger amounts than normal. Absorption of ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(85)90062-0

    authors： Levine S

    更新日期：1985-08-01 00:00:00

  • Performance of HPV E6/E7 mRNA RT-qPCR for screening and diagnosis of cervical cancer with ThinPrep Pap test samples.

    abstract：:Recent research has shown that oncogenic human papillomavirus (HPV) DNA, which is currently used in the screening and diagnosis of cervical cancer, can be detected not only in high-grade cervical lesions, but also in low-grade cervical lesions and normal tissues. For this reason, HPV tests targeting the E6 and E7 mRNA...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.08.004

    authors： Munkhdelger J,Kim G,Wang HY,Lee D,Kim S,Choi Y,Choi E,Park S,Jin H,Park KH,Lee H

    更新日期：2014-10-01 00:00:00

  • Histopathological effects of homocysteine thiolactone on epithelial and stromal tissues.

    abstract：:To study the effects of homocysteine on epithelial and stromal tissues, the free base of homocysteine thiolactone was synthesized and administered to mice. Intraperitoneal and intramuscular doses of 0.2 to 2.0 mg/g are acutely toxic, causing as much as 90% mortality because of intense tissue necrosis at the injection ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(89)90016-6

    authors： McCully KS,Vezeridis MP

    更新日期：1989-10-01 00:00:00

  • Dengue fever and bone marrow myelofibrosis.

    abstract：:Myelofibrosis is characterized by reticulin and/or collagen fibrosis in the bone marrow stroma resulting in secondary cytopenia. In addition to clonal hematologic neoplasms, myelofibrosis may also develop in association with other clinical conditions, including hematological disorders, solid malignancies, Down syndrom...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.07.004

    authors： Qing X,Sun N,Yeh J,Yue C,Cai J

    更新日期：2014-10-01 00:00:00

  • EGFR ligands exert diverging effects on male reproductive organs.

    abstract：:While the EGFR and most of its ligands are expressed in the male reproductive tract, their functions in male reproduction are poorly understood. Interestingly, male transgenic mice overexpressing EGF are sterile, and transgenic mice overexpressing TGFA, another EGFR ligand, show an enlarged coagulation gland (anterior...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2009.11.005

    authors： Schneider MR,Gratao AA,Dahlhoff M,Boersma A,Hrabé de Angelis M,Hoang-Vu C,Wolf E,Klonisch T

    更新日期：2010-02-01 00:00:00

  • Ancylostoma ceylanicum infection in female hamsters: an observation on altered reproductive function.

    abstract：:Reproductive performances of female hamsters were investigated during Ancylostoma ceylanicum (hookworm) infection. Animals having the highest levels of infection (34.96 +/- 1.11 worms) showed degenerative changes in the reproductive system. Ovaries of infected animals contained a few primary or secondary follicles. On...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(92)90043-b

    authors： Gupta S,Srivastava JK,Malaviya B,Katiyar JC

    更新日期：1992-08-01 00:00:00

  • Distribution of N-methyl-D-aspartate receptors (NMDARs) in the developing rat brain.

    abstract：:The N-methyl-D-aspartate receptor (NMDAR), which is one of the glutamate receptors, is considered to have a close relationship to synaptic plasticity in the developing brain. In addition, it is known that the excessive stimulation of NMDARs can trigger neuronal apoptosis. In this study, we examined the distribution of...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/s0014-4800(03)00030-3

    authors： Takai H,Katayama K,Uetsuka K,Nakayama H,Doi K

    更新日期：2003-08-01 00:00:00

  • Experimental strategies to improve in vitro models of renal ischemia.

    abstract：:Ischemia has elicited a great deal of interest among the scientific community due to its role in life-threatening pathologies such as cancer, stroke, acute renal failure, and myocardial infarction. Oxygen deprivation (hypoxia) associated with ischemia has recently become a subject of intense scrutiny. New investigator...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexmp.2007.03.002

    authors： Russ AL,Haberstroh KM,Rundell AE

    更新日期：2007-10-01 00:00:00

  • Respiratory reovirus 1/L induction of diffuse alveolar damage: a model of acute respiratory distress syndrome.

    abstract：:Acute respiratory distress syndrome (ARDS) is a clinical syndrome that is characterized by diffuse alveolar damage usually secondary to an intense host inflammatory response of the lung to a pulmonary or extrapulmonary infectious or noninfectious insult. In this report we describe a unique animal model in which CBA/J ...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2001.2414

    authors： London L,Majeski EI,Paintlia MK,Harley RA,London SD

    更新日期：2002-02-01 00:00:00

  • Estrogen influence on maturational pathway of murine mammary tumor virus: an immunoelectron microscopy study.

    abstract：:Modifications induced by estrogens on hormone-independent murine mammary tumor (MMT) and its main etiological agent, the MMT virus (MMTV), are reported. High doses of estrogens released continuously from silastic capsules delay significantly the development of transplanted tumors into syngeneic hosts. Neoplastic cells...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/0014-4800(89)90053-1

    authors： Peralta Soler A,Aoki A

    更新日期：1989-02-01 00:00:00

  • Assessment of molecular testing in fine-needle aspiration biopsy samples: an experience in a Chinese population.

    abstract：:Fine-needle aspiration biopsy remains the mainstay for preoperative examination of thyroid nodules; however, it does not provide a definite diagnosis in up to 25% of nodules. Considerable studies have been performed to identify molecular markers to resolve this diagnostic dilemma. The aim of this study was to establis...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2014.08.005

    authors： Liu S,Gao A,Zhang B,Zhang Z,Zhao Y,Chen P,Ji M,Hou P,Shi B

    更新日期：2014-10-01 00:00:00

  • Fibrosin: a novel lymphokine in wound healing.

    abstract：:Several growth factors are actively synthesized during wound repair and function to stimulate different cell types involved in the process of healing. Fibrosin is a novel fibrogenic lymphokine that stimulates several biological activities that relate to in vivo scarring. To investigate the role of fibrosin, we used "p...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2001.2402

    authors： Prakash S,Robbins PW

    更新日期：2001-12-01 00:00:00

  • Adhesion molecules as susceptibility factors in spontaneous autoimmune thyroiditis in the NOD-H2h4 mouse.

    abstract：:Mononuclear cell infiltration of the thyroid is a prominent feature of chronic lymphocytic thyroiditis. Adhesion molecules play a major role in determining the localization of inflammatory mononuclear cells in the thyroid. Previous reports from animal models and human studies have described the thyroidal expression of...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1006/exmp.2002.2470

    authors： Bonita RE,Rose NR,Rasooly L,Caturegli P,Burek CL

    更新日期：2002-12-01 00:00:00

  • Quantitative evaluation of SPRR3 expression in esophageal squamous cell carcinoma by qPCR and its potential use as a biomarker.

    abstract：:Esophageal squamous cell carcinoma (ESCC) is highly fatal due to late diagnosis and inefficient treatment. Early disease detection could improve diagnosis and patient survival. Esophageal squamous epithelial cells express SPRR3, a member of the small proline-rich protein family, which is downregulated in ESCC. Therefo...

    journal_title：Experimental and molecular pathology

    pub_type： 杂志文章

    doi：10.1016/j.yexmp.2011.06.006

    authors： de A Simão T,Souza-Santos PT,de Oliveira DS,Bernardo V,Lima SC,Rapozo DC,Kruel CD,Faria PA,Ribeiro Pinto LF,Albano RM

    更新日期：2011-10-01 00:00:00