Abstract:
:In the presence of nerve growth factor (NGF) PC12 pheochromocytoma cells develop properties of sympathetic neurons and extend long microtubule-containing neurites. PC12 cell microtubule protein was isolated using an assembly and disassembly procedure, either directly from an unlabeled cell extract or by copolymerization of a [35S]-methionine-labeled cell extract with rat brain microtubule proteins. Microtubule proteins of PC12 cells treated and untreated with NGF did not reveal any differences as analyzed by SDS-PAGE. The major constituents were the tubulin dimer and microtubule-associated proteins, mainly one high molecular weight component (HMW2) (Mr = 290,000), which resembles by several criteria the high molecular weight protein MAP2 of rat brain microtubule protein. PC12 cell microtubule protein contained intrinsic cAMP-dependent and cAMP-independent protein kinase activity. In vitro phosphorylation experiments indicated that, unlike in rat brain microtubule preparations, both subunits of the tubulin dimer were substrate for the endogenous kinase activity. The addition of cAMP to the incubation medium exerted a slight increase in phosphorylation of the HMW2 protein and an 80,000 polypeptide, and most effectively the phosphorylation of a Mr = 62,000 peptide was increased. This component could be identified as tyrosine hydroxylase by indirect immunoprecipitation procedures. Thus, the in vitro phosphorylation pattern of PC12 cell microtubule protein differed substantially from the one obtained from rat brain microtubule preparations.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Richter-Landsberg C,Landreth GE,Shooter EMdoi
10.1159/000112330subject
Has Abstractpub_date
1983-01-01 00:00:00pages
32-44issue
1eissn
0378-5866issn
1421-9859journal_volume
6pub_type
杂志文章abstract::Prenatal fever could result in brain function impairments in the offspring. The present study investigated the effect of interleukin-6 (IL-6)-induced maternal fever on the offspring and the involvement of connexin 36 in this process. Pregnant C57BL/6J mice were injected with IL-6 on gestational day 15. The levels of i...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000499735
更新日期:2019-01-01 00:00:00
abstract::Neurite outgrowth is dependent on the presence in neurites of assembled microtubules which consist of polymerized tubulin to which microtubule-associated proteins (MAPs) are bound. This study adds to previous evidence of the similarity between the high molecular weight MAPs, MAP1X, MAP1B and MAP5, and explains the dif...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111318
更新日期:1993-01-01 00:00:00
abstract::Insulin binding was measured on crude mitochondrial or plasma membranes prepared from different rat brain regions during postnatal development. In the cerebral cortex and brainstem, insulin binding decreased 60-70% between birth and the adult period. In the cerebellum, insulin binding doubled in the first 10 postnatal...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111863
更新日期:1990-01-01 00:00:00
abstract::Ethanol inhibition of L1-mediated cell adhesion may contribute to the spectrum of neurological, behavioral and morphological abnormalities associated with prenatal ethanol exposure. We showed previously that the neuroprotective peptides NAPVSIPQ (NAP) and SALLRSIPA (SAL) antagonize ethanol inhibition of L1 adhesion an...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000084528
更新日期:2005-01-01 00:00:00
abstract::The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accord...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111612
更新日期:1987-01-01 00:00:00
abstract::The norepinephrine content of hypothalamus in vehicle-treated control rats gradually increases between days 16 and 45 of life. Norepinephrine contents of the hypothalamus and mesencephalon of the thyroxine-treated (10 microgram/g b.w., s.c., days 8-10) animals also increase between days 16 and 21. Mesencephalic and hy...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112377
更新日期:1980-01-01 00:00:00
abstract::A leading hypothesis of the cause of neuronal death in Huntington's disease (HD) is excitotoxicity, in which subpopulations of striatal neurons are hypersensitive to glutamate release due to changes in postsynaptic N-methyl-D-aspartate receptors (NMDARs). In the present study we used RT-PCR methods on single cells and...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000091921
更新日期:2006-01-01 00:00:00
abstract::Neurotransmitters play important roles in neurogenesis; in particular, acetylcholine and serotonin may regulate neurite elongation. Acetylcholine may also activate transcription factors such as early growth response protein 1 (EGR-1), which plays a role in neurite extension. N18TG2 neuroblastoma cells (which do not pr...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000354423
更新日期:2013-01-01 00:00:00
abstract::Chick embryo retina neurons in culture extend neurites and form synapses. Seven different culture substrates were compared to determine which culture surface would produce the best neuronal survival and neurite extension. The order of survival of retina neurons during 7 days of culture is salt-precipitated collagen gr...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112705
更新日期:1982-01-01 00:00:00
abstract::Myelinogenesis in different brain parts of trout was studied comparatively by means of histochemistry and biochemistry. A more detailed approach, including ultrastructural analysis, to characterize this important differentiation process, particularly in the optic tectum, revealed a good correlation between the structu...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112777
更新日期:1981-01-01 00:00:00
abstract::Early developmental processes, such as metabolic programming, can provide cues to an organism, which allow it to make modifications that are predicted to be beneficial for survival. Similarly, social play has a multifaceted role in promoting survival and fitness of animals. Play is a complex behavior that is greatly i...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000444015
更新日期:2016-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is predominantly a clinical problem of young persons, resulting in chronic cognitive and behavioral deficits. Specifically, the physiological response to a diffuse biomechanical injury in a maturing brain can clearly alter normal neuroplasticity. To properly evaluate and investigate develo...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000094163
更新日期:2006-01-01 00:00:00
abstract::To define the effects of neurotrophins on oligodendrocytes, we monitored NGF, BDNF and NT-3 actions on basal forebrain (BF) and cortical populations. NGF, BDNF and NT-3 applied to BF oligodendrocytes elicited increases in expression of myelin basic protein (MBP) and enhanced the numbers of MBP+ cells, without affectin...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000072261
更新日期:2003-03-01 00:00:00
abstract::A glia-derived neurite-promoting factor has been purified from medium conditioned by C6 rat glioma cells. It induces neurite outgrowth in cultured mouse neuroblastoma cells and inhibits granule cell migration in explants of mouse cerebellum. This factor is a potent serine protease inhibitor which has recently been sho...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111951
更新日期:1988-01-01 00:00:00
abstract::The incidence of stroke in children is 2.4 per 100,000 person-years and results in long-term motor and cognitive disability. In ischemic stroke, white matter (WM) is frequently injured, but is relatively understudied compared to grey matter injury. Previous research suggests that the cellular response to WM ischemic i...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000496200
更新日期:2019-03-12 00:00:00
abstract::Previous studies have demonstrated that exposure to ethanol during development delays the rate at which axons in certain central nervous system tracts acquire myelin. This delay appears to be related to an alteration in oligodendrocyte function and not to an aberrancy in axon size or number. The present study was desi...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112154
更新日期:1991-01-01 00:00:00
abstract::There is an important unmet need to further improve the outcome of neonatal encephalopathy in term infants. Meta-analyses of large controlled trials now suggest that maternal magnesium sulfate (MgSO4) therapy is associated with a reduced risk of cerebral palsy and gross motor dysfunction after premature birth, but tha...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000362206
更新日期:2014-01-01 00:00:00
abstract::Transient early cerebral laminar organization resulting from normal developmental events has been revealed in human beings through histology and imaging studies. DTI studies have postulated that the fractional anisotropy (FA)-based differentiation of different laminar structures reflects both differing cellular densit...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000229500
更新日期:2009-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is the leading cause of death and disability in children. Evidence-based guidelines for the management of this population are available; however, the data highlight significant deficiencies with few treatment standards or guidelines. Considering the limited availability of resources, it is...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000094153
更新日期:2006-01-01 00:00:00
abstract::In adult rats, kainic acid-induced status epilepticus markedly reduces GluR2 (the alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid, AMPA subunit that limits Ca2+ permeability), receptor mRNA in the vulnerable CA3 and may contribute to delayed neurodegeneration. In rat pups resistant to kainate seizure-induced...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111257
更新日期:1997-01-01 00:00:00
abstract::Quaking is a neurological mutation leading to pleiotropic phenotypic expression, the most prominent being disturbed myelin formation in the central nervous system (CNS) with minor abnormalities in the peripheral nervous system. Previous immunochemical measurements of glial fibrillary acidic protein (GFAP) revealed a m...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111682
更新日期:1992-01-01 00:00:00
abstract::Cortical dysplasia (CD) is now recognized as one of the major causes of pediatric focal neocortical epilepsy, and surgical procedures have been considered early in life. However, the mechanisms involved in seizure generation and intractability in these patients are still unknown. We analyzed with immunocytochemistry f...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017406
更新日期:1999-11-01 00:00:00
abstract::Adult GM2 gangliosidosis is a rare autosomal recessive disease with widely varying neurological and psychiatric manifestations. It is caused by marked deficiency, but not total absence, of beta-hexosaminidase (Hex) A, due to a single base change in the alpha-subunit gene of Hex, resulting in a substitution of Ser for ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112200
更新日期:1991-01-01 00:00:00
abstract::The kinetics and sodium-dependence of gamma-aminobutyric acid (GABA), hypotaurine and taurine uptake were analyzed in cerebral slices prepared from mice of different postnatal ages. The maximal velocities of transport (V) and the Km constants for the high-affinity uptake all decreased with age, whereas the development...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112354
更新日期:1983-01-01 00:00:00
abstract::Choline acetyltransferase (ChAT), the biosynthetic enzyme for acetylcholine metabolism, is expressed in a human cholinergic neuroblastoma cell line; MC-IXC. We demonstrate that ChAT activity is regulated in this cell line by cell density. It is believed that the mechanism of stimulation of enzyme activity involves cel...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111975
更新日期:1988-01-01 00:00:00
abstract::Early research in the Vannucci laboratory prior to 1981 focused largely on brain energy metabolism in the developing rat. At that time, there was no experimental model to study the effects of perinatal hypoxia-ischemia in the rodent, despite the tremendous need to investigate the pathophysiology of perinatal asphyxial...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000085978
更新日期:2005-03-01 00:00:00
abstract::When cells from the cerebellum of late-gestation rats were grown at low density (50-100 cells/mm2) in a three-dimensional culture system, they rapidly expressed morphological and electrophysiological properties of neurons. Growth and differentiation of the population of solitary neurons as a whole was statistically as...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111645
更新日期:1992-01-01 00:00:00
abstract::The spontaneously immortalized S16 Schwann cell line expresses higher levels of myelin-associated glycoprotein (MAG) and PO glycoprotein and their messenger RNAs when grown at high density than at low density [Sasagasako et al: J Neurochem 1996;66:1432-1439]. This up-regulation of myelin protein expression at high den...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017409
更新日期:1999-01-01 00:00:00
abstract::Systemic creatine (Cr) supplementation increases brain phosphocreatine (PCr) and prevents hypoxic seizures in 15-day-old rabbits. Between 5 and 30 days of age during normal development, rabbit gray matter mitochondrial creatine kinase (Mi-CK) increases 400% while cytosolic CK (BB-CK) increases 60%. In white matter, bo...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017473
更新日期:2000-09-01 00:00:00
abstract::We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111893
更新日期:1989-01-01 00:00:00