Abstract:
:When cells from the cerebellum of late-gestation rats were grown at low density (50-100 cells/mm2) in a three-dimensional culture system, they rapidly expressed morphological and electrophysiological properties of neurons. Growth and differentiation of the population of solitary neurons as a whole was statistically assessed at 6, 12, 18, 24, 48 and 72 h using data obtained from image analysis. Mean length of axons and dendrites increased 17- and 220-fold, respectively, from 6 to 72 h. Average number of branch points rose 35-fold. Other indices of complexity increased 2- to 3-fold. Whole-cell voltage clamp revealed that as early as 24-30 h the cells displayed a tetrodotoxin-sensitive Na+ current (INa), a nifedipine-sensitive high-threshold Ca2+ current, a Ni(2+)-sensitive low-threshold Ca2+ current, and two voltage-dependent K+ outward currents consisting of a 4-aminopyridine-sensitive fast transient outward current and a CsCl-sensitive slow delayed component. These observations correlate closely with voltage-activated currents previously recorded in neonatal or young rat cerebellum, and demonstrate that the culture model is useful for analyzing the early rapid growth, differentiation and intrinsic ionic currents of these neurons as single cells.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Coates PW,Fermini B,Strahlendorf JC,Strahlendorf HKdoi
10.1159/000111645keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
35-43issue
1eissn
0378-5866issn
1421-9859journal_volume
14pub_type
杂志文章abstract::Maternal infection during pregnancy increases the risk of neurobehavioral problems in offspring. Evidence from rodent models indicates that the maternal immune response to infection can alter fetal brain development, particularly in the hippocampus. However, information on the effects of maternal viral infection on fe...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000486850
更新日期:2018-01-01 00:00:00
abstract::Developmental expression of neutral monoglycosylceramides (MGCs) has been examined in rat brain from embryonic day 15 (E15) to postnatal day 30 (P30) and adulthood. To this point, glucosylceramide (GlcCer) is the only MGC that has been characterized in embryonic brain. Galactosylceramide (GalCer) appears at P1, increa...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111201
更新日期:1997-01-01 00:00:00
abstract::The myelin-associated glycoprotein (MAG) in primary cultures of oligodendrocytes is subject to phosphorylation in the absence of neurons. The positive response of this phosphorylation to vanadate suggests that one of the modified sites in MAG is phosphotyrosine. We found that phosphorylation was enhanced by brief trea...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112192
更新日期:1991-01-01 00:00:00
abstract::The gene responsible for the audiogenic seizure (AGS) phenotype in Frings mice, which was identified and originally designated Mass1, is now referred to as Mgr1. Although the function of the gene product is not known, the expression pattern suggests a role in the developing CNS. Hearing impairment is often observed in...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000086712
更新日期:2005-01-01 00:00:00
abstract::Neurite outgrowth is dependent on the presence in neurites of assembled microtubules which consist of polymerized tubulin to which microtubule-associated proteins (MAPs) are bound. This study adds to previous evidence of the similarity between the high molecular weight MAPs, MAP1X, MAP1B and MAP5, and explains the dif...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111318
更新日期:1993-01-01 00:00:00
abstract::We investigated the effects of perinatal hypoxia-ischemia (HI) on brain injury and neurological functional outcome at postnatal day (P)30 through P90. HI was induced by exposing P9 mice to 8% O(2) for 55 min using the Vannucci HI model. Following HI, mice were treated with either vehicle control or Na(+)/H(+) exchange...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000328430
更新日期:2011-01-01 00:00:00
abstract::Rabbit central (CNS) and peripheral nervous system (PNS) myelin, as well as nonmyelinating pig oligodendrocytes in culture, were extracted at 0-4 degrees C with the nonionic detergent Triton X-114. The solubilized proteins were partitioned into the detergent-rich and detergent-depleted (aqueous) phases that form upon ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111935
更新日期:1989-01-01 00:00:00
abstract::Using Western blot and immunocytochemistry to detect protein, and in situ hybridization and Northern blot to detect RNA, we studied the expression of Na,K-ATPase subunit isoforms in cultured astrocytes and oligodendrocytes in vitro, and in glial cells in the brain. We found that astrocytes in culture express alpha 1 a...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111422
更新日期:1996-01-01 00:00:00
abstract::The spontaneously immortalized S16 Schwann cell line expresses higher levels of myelin-associated glycoprotein (MAG) and PO glycoprotein and their messenger RNAs when grown at high density than at low density [Sasagasako et al: J Neurochem 1996;66:1432-1439]. This up-regulation of myelin protein expression at high den...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017409
更新日期:1999-01-01 00:00:00
abstract::The postnatal developmental expression and the distribution of the glutamate transporters (GLAST, GLT-1 and EAAC1) were analyzed in rat vestibular nuclei (VN), at birth and during the following 4 weeks. Analyses were performed using reverse transcriptase-polymerase chain reaction and immunoblotting of GLAST, GLT-1 and...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000073510
更新日期:2003-09-01 00:00:00
abstract::Preadolescent animals display protracted hormonal stress responses mediated by the hypothalamic-pituitary-adrenal (HPA) axis compared to adults. Though the mechanisms that underlie this shift in stress reactivity are unknown, reduced glucocorticoid-dependent negative feedback on the HPA axis has been posited to contri...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000362873
更新日期:2014-01-01 00:00:00
abstract::The effect of undernutrition on the activity of glutamate decarboxylase (GAD) and choline acetyltransferase (ChAc) (markers for the GABA-ergic and the cholinergic transmitter system, respectively) was studied in various parts of the rat brain at the age of 10, 15 and 21 days, and at day 54 following 33 days of rehabil...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112551
更新日期:1978-01-01 00:00:00
abstract::Three experimental approaches were used to examine the maturation of N-methyl-D-aspartate (NMDA) receptors in the neostriatum and compare their developmental profile to that of the non-NMDA receptors [alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate (KA)]. The first, and least conventional ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017310
更新日期:1998-01-01 00:00:00
abstract::Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellum extracts analyzed by two-dimensional gels, using antipeptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence o...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111452
更新日期:1996-01-01 00:00:00
abstract::Birth asphyxia can result in sensory impairment, learning and memory deficits without gross brain injury and severe motor deficits. We developed a model of birth asphyxia resulting in mild neurological injury and cognitive impairment using a long-gestation species with precocial fetal development. Spiny mice (Acomys c...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000251907
更新日期:2009-01-01 00:00:00
abstract::Plexin is a type I membrane protein which was identified in Xenopus nervous system by hybridoma technique. Molecular cloning studies demonstrated that the extracellular segment of the plexin protein possesses three internal repeats of cysteine cluster which are homologous to the cysteine-rich domain of the c-met proto...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111192
更新日期:1997-01-01 00:00:00
abstract::In the development of an integrated approach to study metabolic compartmentation and regulation in brain, we have emphasized the importance, versatility, and need to exploit the recent methodological advances in (1) NMR spectroscopy, (2) primary cultures of neurons and glial cells, and (3) subcellular fractionation (e...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111334
更新日期:1993-01-01 00:00:00
abstract::In this study, the proliferative activity of cultured chick neuroblasts exposed to high or low concentrations of fetal calf serum (FCS) was investigated. Different experimental evidences showed that the first 2-3 days of culture correspond to a period of neuronal proliferation in our culture conditions, whereas contam...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112344
更新日期:1983-01-01 00:00:00
abstract::Autism spectrum disorder (ASD) comprises a group of neurodevelopmental disorders characterized by social deficits and stereotyped behaviors. While several theories have emerged, the pathogenesis of ASD remains unknown. Although studies report dopamine signaling abnormalities in autistic patients, a coherent dopamine h...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000478725
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Neonatal hypoxic-ischemic (HI) encephalopathy occurs in 1-4 per 1,000 live term births and can cause devastating neurodevelopmental disabilities. Currently, therapeutic hypothermia (TH) is the only treatment with proven efficacy. Since TH is associated with decreased cerebral metabolism and cerebral blood fl...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000381704
更新日期:2015-01-01 00:00:00
abstract::The myelin-associated glycoprotein (MAG) is a 100 K-dalton, integral membrane glycoprotein containing 30% carbohydrate that is in central and peripheral myelin sheaths. Recent research on its function during normal myelinogenesis and possible roles in the pathology of demyelinating diseases is reviewed. Emphasis is pl...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000112356
更新日期:1983-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is the leading cause of death and disability in children. Evidence-based guidelines for the management of this population are available; however, the data highlight significant deficiencies with few treatment standards or guidelines. Considering the limited availability of resources, it is...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000094153
更新日期:2006-01-01 00:00:00
abstract::We have previously shown that a single intracranial injection of apotransferrin (aTf) in neonatal rats produces an accelerated mylinogenesis and increases the expression of certain myelin proteins such as myelin basic protein (MBP). In the present work, we studied the effects of aTf upon oligodendrocyte progenitor cel...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000064945
更新日期:2002-01-01 00:00:00
abstract::Neuroblastoma is a childhood cancer caused by the transformation of sympathoadrenal progenitors. By following the formation of tumors in homozygous TH-MYCN mice, an established mouse model of neuroblastoma, we were able to capture transformed cells prior to the formation of large, vascularized tumors in order to deter...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000365281
更新日期:2014-01-01 00:00:00
abstract::Uncertainty reigns over whether or not glutamate uptake in astrocytes leads to strong stimulation of glucose utilization, measured as accumulation of radioactive deoxyglucose-6-phosphate. This is an important issue, not only because glutamate is the major excitatory transmitter, but also because it has been postulated...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017329
更新日期:1998-01-01 00:00:00
abstract::While extracellular signals play a major role in brain neurogenesis, little is known about the cell cycle machinery underlying mitogen stimulation of precursor proliferation. Current models suggest that the D cyclins function as primary sensors of extracellular mitogens. Here we define the mechanisms by which basic fi...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000082137
更新日期:2004-03-01 00:00:00
abstract::Developing neurons synthesize substantial quantities of membrane phospholipids in producing new synapses. We investigated the effects of maternal uridine (as uridine-5'-monophosphate) and docosahexaenoic acid supplementation on pups' brain phospholipids, synaptic proteins and dendritic spine densities. Dams consumed n...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000193394
更新日期:2009-01-01 00:00:00
abstract::Insulin binding was measured on crude mitochondrial or plasma membranes prepared from different rat brain regions during postnatal development. In the cerebral cortex and brainstem, insulin binding decreased 60-70% between birth and the adult period. In the cerebellum, insulin binding doubled in the first 10 postnatal...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111863
更新日期:1990-01-01 00:00:00
abstract::Systemic creatine (Cr) supplementation increases brain phosphocreatine (PCr) and prevents hypoxic seizures in 15-day-old rabbits. Between 5 and 30 days of age during normal development, rabbit gray matter mitochondrial creatine kinase (Mi-CK) increases 400% while cytosolic CK (BB-CK) increases 60%. In white matter, bo...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017473
更新日期:2000-09-01 00:00:00
abstract::Cerebral metabolism is known to be highly dependent upon glucose utilisation. In the neonate, severe hypoglycaemia is now recognized to be associated with subsequent adverse neurodevelopment and in extremis glial and neuronal cell death. However, the level at which hypoglycaemia becomes clinically important is not wel...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000112124
更新日期:1994-01-01 00:00:00