Abstract：
:Autoradiograms of mouse pituitaries were prepared 30 min after injection of 3H-dexamethasone (3HDM), 3H-corticosterone (3HB) and 3H-cortexolone (3HS) either alone or in the presence of competing unlabelled steroids. 3H-dexamethasone accumulated in cell nuclei of both the pars distalis and the pars nervosa but not in those of the pars intermedia. This preferential accumulation (nuclear/cytoplasmic grain density, 4 : 1) was abolished by the concurrent administration of excess dexamethasone. 3H-corticosterone, to a much less marked extent than 3H-dexamethasone, accumulated in cell nuclei of the pars distalis but not in those of the pars intermedia and the pars nervosa. Excess unlabelled corticosterone diminished nuclear grain density in the pars distalis. After 3-h-cortexolone injection, preferential nuclear uptake was not observed. In a second series of experiments, excess dexamethasone (10 x, 100 x), corticosterone (100 x, 300 x) and cortexolone (100 x, 300 x) administered with 3H-dexamethasone were without effect on cytoplasmic grain density but totally abolished preferential nuclear accumulation. Parallel biochemical studies on kidney cytoplasmic preparations from the same animals showed no differences in total cytoplasmic radioactivity between treatments but marked differences in cytoplasmic bound 3H-dexamethasone. The results demonstrate: i) that dexamethasone binds specifically to cell nuclei of the pars distalis and the pars nervosa and that this nuclear concentration is abolished by competing corticosterone and cortexolone as well as dexamethasone; ii) that corticosterone localizes in cell nuclei of the pars distalis but much less markedly than dexamethasone; iii) that cortexolone fullfils the criteria of a glucocorticoid antagonist at the pituitary cell level.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Coutard M,Osborne-Pellegrin MJ,Funder Jdoi
10.1007/BF00236423subject
Has Abstractpub_date
1979-08-01 00:00:00pages
311-22issue
2eissn
0302-766Xissn
1432-0878journal_volume
200pub_type
杂志文章abstract：:To gain a better understanding of the neuroplasticity of sympathetic neurons during postnatal ontogenesis, the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity was studied in sympathetic preganglionic neurons (SPN) in the spinal cord (Th2 segment) of female Wistar rats at different ages (newborn,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2925-8
更新日期：2019-02-01 00:00:00
abstract：:A striking feature of vestibular hair cells is the polarized arrangement of their stereocilia as the basis for their directional sensitivity. In mammals, each of the vestibular end organs is characterized by a distinct distribution of these polarized cells. We utilized the technique of post-fixation transganglionic ne...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0944-1
更新日期：2010-05-01 00:00:00
abstract：:Identified dorsal unpaired median (DUM) neurones of the locust Locusta migratoria were stained intracellularly with large amounts of cobalt to reveal their extensive peripheral branching patterns. Two neurones of the suboesophageal ganglion were studied as well as several neurones of thoracic ganglia. The peripheral b...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050970
更新日期：1997-12-01 00:00:00
abstract：:Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318364
更新日期：1995-06-01 00:00:00
abstract：:The pinealocytes of the pig contain conspicuous dense bodies, the nature and role of which are not yet fully elucidated. The aim of this study was to demonstrate whether or not these structures are involved in the secretion process. The tannic acid-Ringer incubation (TARI)-method, which allows a clear-cut ultrastructu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313977
更新日期：1991-05-01 00:00:00
abstract：:In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0543-y
更新日期：2008-03-01 00:00:00
abstract：:Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051072
更新日期：1998-06-01 00:00:00
abstract：:N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214676
更新日期：1987-10-01 00:00:00
abstract：:The molecular cloning of a cDNA fragment synthesised from rat duodenal mRNA coding for cholecalcin (calbindin), a 9000 Mr vitamin D-induced calcium-binding protein (CaBP), has been previously described. DNA/RNA hybridisation assays have been used to examine CaBP mRNA production in the uterine horns and duodena of preg...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216546
更新日期：1987-01-01 00:00:00
abstract：:Placing the clawed toad Xenopus laevis on a black background stimulates the melanotrope cells in the pars intermedia of the pituitary gland to release proopiomelanocortin (POMC)-derived peptides, including alpha-MSH and N-acetyl-beta-endorphin. In this study three types of secretory granules, electron-dense (approxima...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00314557
更新日期：1993-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225762
更新日期：1975-07-23 00:00:00
abstract：:Osteoarthritis (OA) is the most common joint disease with an unsatisfactory therapy outcome and characterized by the degradation of articular cartilage and synovial inflammation. Here, we isolated bone marrow mesenchymal stem cells (BMSCs) from rat's bone marrow and BMSC-derived exosome (BMSCs-Exo) from BMSCs successf...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03319-1
更新日期：2021-01-06 00:00:00
abstract：:Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222069
更新日期：1975-01-01 00:00:00
abstract：:Intercellular bridges (IBs) connecting the cytoplasms of a defined type of defective germ cell division ("arrested mitoses") in male and female gonads of the immature golden hamster were studied by electron microscopy. In both sexes, such cells appear at the time when germ cells switch from mitotic proliferation to th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0523-1
更新日期：2002-04-01 00:00:00
abstract：:The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319612
更新日期：1992-08-01 00:00:00
abstract：:The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1889-6
更新日期：2014-09-01 00:00:00
abstract：:The lobula descending neuron (LDN) of dipterous insects is a unique nerve cell (one on each side of the brain) that projects directly from the lobula complex of the optic lobes to neuropil in thoracic ganglia. In the supraoesophageal ganglia the LDN has two prominent groups of branches of which at least one is dendrit...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218365
更新日期：1982-01-01 00:00:00
abstract：:Ultrastructural post-embedding immuno-gold techniques were applied to the supraoptic nucleus and the neurohypophysis of mice and rats. The primary antibodies were three different monoclonal antineurophysins, used in protein A-gold and immunoglobulin-gold procedures. Conventional plastic embedding as well as hydrophili...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221868
更新日期：1986-01-01 00:00:00
abstract：:Stem cells are an attractive cellular source for regenerative medicine and tissue engineering applications due to their multipotency. Although the elasticity of the extracellular matrix (ECM) has been shown to have crucial impacts in directing stem cell differentiation, it is not the only contributing factor. Many res...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-015-2190-z
更新日期：2015-09-01 00:00:00
abstract：:The pattern of innervation on individual iliotibialis muscle fibres from axolotl (Ambystoma mexicanum) has been investigated histologically and electrophysiologically. These polyneural fibres were found to be innervated on average at five end plate sites. The sites were distributed irregularly along each fibre. Averag...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219663
更新日期：1978-01-09 00:00:00
abstract：:To investigate the control of the timing in the epithelio-mesenchymal transformation of the neural crest into a migrating population, neural anlagen (neural tube plus crest) were isolated from 2-day quail embryos by proteases in the presence of CA++ and explanted onto substrates favourable for neural crest cell migrat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218011
更新日期：1985-01-01 00:00:00
abstract：:To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233727
更新日期：1980-01-01 00:00:00
abstract：:Spermatozoa from the cauda epididymidis of gossypol-treated rats exhibit distinctive departures from the morphology of spermatozoa from control rats: wrinkled and disorganized cell membrane in the head and tail regions, cell membrane missing from segments of the tail midpiece and principal piece regions, malformed hea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210003
更新日期：1981-01-01 00:00:00
abstract：:Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050531
更新日期：1996-02-01 00:00:00
abstract：:Colitis is associated with alterations in electrolyte and water transport. These changes give rise to some of the symptoms experienced by patients with colitis. Alterations in fluid flux may also contribute to increased susceptibility to mucosal injury. Recently, endogenous water channel proteins (aquaporins; AQPs), h...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0932-4
更新日期：2004-11-01 00:00:00
abstract：:Chronic tinnitus (ringing of the ears) is a medically untreatable condition that reduces quality of life for millions of individuals worldwide. Most cases are associated with hearing loss that may be detected by the audiogram or by more sensitive measures. Converging evidence from animal models and studies of human ti...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1992-8
更新日期：2015-07-01 00:00:00
abstract：:An investigation of the stereospecificity of beta-adrenergic insulin release, its relation to alpha-adrenergic blockade and the adrenergic innervation of the pancreatic islets was performed in the mouse. It was observed that in vivo beta-adrenergic stimulation of insulin release by isopropylnoradrenaline was stereospe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221602
更新日期：1978-10-06 00:00:00
abstract：:Sacciform cells containing an acidophilic, proteinaceous secretion, were identified in the epidermis of the brown trout and Arctic char. This cell type increased in number during the chronic stages of infestation by the ectoparasitic flagellate, Ichthyobodo sp., in immature brown trout, and decreased during sexual mat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218307
更新日期：1987-02-01 00:00:00
abstract：:Tenidap is a structurally novel antirheumatic agent with anti-inflammatory and analgesic properties. Previous studies have shown that tenidap is able to inhibit the production and action of cytokines such as interleukin-1, interleukin-6 (IL-6) and tumour necrosis factor alpha. However, the mechanisms by which tenidap ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050810
更新日期：1997-05-01 00:00:00
abstract：:The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218204
更新日期：1987-05-01 00:00:00
