Abstract：
:Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR antibodies. The labeling pattern was abolished by preincubation of anti-CFTR antibodies with the immunogen or when non-immune IgG was used in place of anti-CFTR antibodies. These results support other studies that suggest that alterations in goblet cell function may be involved in the intestinal abnormalities associated with cystic fibrosis.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Hayden UL,Carey HVdoi
10.1007/s004410050531subject
Has Abstractpub_date
1996-02-01 00:00:00pages
209-13issue
2eissn
0302-766Xissn
1432-0878journal_volume
283pub_type
杂志文章abstract：:Exposure to maternal diabetes during fetal growth is a risk factor for the development of type II diabetes (T2D) in later life. Discovery of the mechanisms involved in this association should provide valuable background for therapeutic treatments. Early embryogenesis involves epigenetic changes including histone modif...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2583-2
更新日期：2017-06-01 00:00:00
abstract：:The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1889-6
更新日期：2014-09-01 00:00:00
abstract：:Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labele...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222243
更新日期：1983-01-01 00:00:00
abstract：:The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222230
更新日期：1983-01-01 00:00:00
abstract：:Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217166
更新日期：1985-01-01 00:00:00
abstract：:Ultrastructural aspects of hypertrophic chondrocytes in hamster and mouse epiphysial cartilage were examined in relation to their metabolic activities. With the hypertrophic change, cytoplasmic vacuolization proceeded leaving the partially intact endoplasmic reticulum (ER). In the hypertrophic cells, cytoplasmic hyalu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900082
更新日期：1999-11-01 00:00:00
abstract：:In previous studies the differentiation of the epithelium in the human hard palate has been described stereologically using parameters expressed per unit tissue volume. Since single epithelial cells represent the true biological units of this tissue, it became necessary to estimate the absolute size of such cells in o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216577
更新日期：1981-01-01 00:00:00
abstract：:In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238295
更新日期：1983-01-01 00:00:00
abstract：:By means of a preembedding immuno-electron-microscopic technique, a large number of nerve endings containing a substance related to human growth hormone-releasing factor (hGRF) have been demonstrated in the paraventricular nucleus of the guinea pig. They made synaptic contacts primarily with dendritic shafts: 80% of t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01239984
更新日期：1987-04-01 00:00:00
abstract：:Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209276
更新日期：1978-05-29 00:00:00
abstract：:A special cell type was identified in the mucosal epithelium of the large intestine of the tadpole of the bullfrog, Rana catesbeiana. It is a slender, columnar cell, with a dark, basally situated nucleus. By electron microscopy the cell displays prominent bundles of filaments emerging from each microvillus and extendi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213817
更新日期：1983-01-01 00:00:00
abstract：:The protein synthesis inhibitor cycloheximide (Chx) suppresses prolactin-induced beta-casein gene expression in the mammary epithelial cell line COMMA-D. As the mechanism underlying this effect is unclear, the effects of protein synthesis inhibitors on interactions of transcription factors with the beta-casein promote...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0672-2
更新日期：2003-02-01 00:00:00
abstract：:Homeobox genes encode transcription factors that regulate embryonic development programs including organogenesis, axis formation and limb development. Previously, we identified and cloned a mouse double homeobox gene, Duxbl, whose homeodomain exhibits the highest identity (67 %) to human DUX4, a candidate gene of faci...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1974-x
更新日期：2014-11-01 00:00:00
abstract：:Two different monoclonal antibodies raised against choline acetyltransferase were used, together with preembedding immunocytochemical techniques, to visualize the possible cholinergic innervation of the supraoptic and paraventricular nuclei of the rat hypothalamus. Light microscopy confirmed the presence of a group of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220024
更新日期：1988-10-01 00:00:00
abstract：:Connective tissue microfibrils were observed in tissues prepared with methods believed to minimize the loss of tissue components. The eyes of C57BL/6J mice were fixed with glutaraldehyde followed by either freeze substitution, or embedding in glycol methacrylate, a water-miscible embedding medium, after limited or no ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318486
更新日期：1995-02-01 00:00:00
abstract：:The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03197-7
更新日期：2020-11-01 00:00:00
abstract：:From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1339-2
更新日期：2012-03-01 00:00:00
abstract：:The fine structure, nature and fate of the components of the nucleolus were studied in young (steps 1, 2), intermediate (steps 3, 4, 5) and mature spermatids (steps 6, 7, 8) of man and monkey, by use of several cytochemical techniques (alcoholic PTA; sodium tungstate: EDTA; HAPTA; nuclease-gold complexes; NOR silver s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305735
更新日期：1991-04-01 00:00:00
abstract：:Neuropeptide F in invertebrates is a homolog of neuropeptide Y in mammals and it is a member of FMRFamide-related peptides. In arthropods, such as insects, there are two types of neuropeptide F comprising long neuropeptide F (NPF) and short neuropeptide F (sNPF). Both NPFs are known to play a crucial role in the regul...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-02999-8
更新日期：2019-06-01 00:00:00
abstract：:Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219019
更新日期：1986-01-01 00:00:00
abstract：:Previous studies have indicated the importance of basement membrane components both for cellular differentiation in general and for the barrier properties of cerebral microvascular endothelial cells in particular. Therefore, we have examined the expression of basement membrane proteins in primary capillary endothelial...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0604-1
更新日期：2002-10-01 00:00:00
abstract：:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214574
更新日期：1985-01-01 00:00:00
abstract：:Perinatal changes in the appearance of intramembranous particles (IMPs) of microvilli of enterocytes were analyzed quantitatively. In both the jejunum and the ileum, the IMP density on the P-face showed no significant changes from day 17 of gestation to day 5 of postnatal life. It increased between day 5 and day 12, r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212224
更新日期：1983-01-01 00:00:00
abstract：:The median eminence/pituitary stalk represents the final common pathway for fibers from neurons that project to the pituitary gland. We have used the lipophilic fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) to determine the location of neurons projecting to the median emin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00304513
更新日期：1995-04-01 00:00:00
abstract：:Electron-dense granules (EDGs) are singular structures found in the tissues of several vertebrate and invertebrate organisms. Two types of EDGs were observed in hepatopancreatic cells of the crab Ucides cordatus: (1) a non-mineralized EDG, found mainly inside vacuoles, which reacted positively to acid phosphatase and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0646-4
更新日期：2003-01-01 00:00:00
abstract：:Osteoarthritis (OA) is the most common joint disease with an unsatisfactory therapy outcome and characterized by the degradation of articular cartilage and synovial inflammation. Here, we isolated bone marrow mesenchymal stem cells (BMSCs) from rat's bone marrow and BMSC-derived exosome (BMSCs-Exo) from BMSCs successf...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03319-1
更新日期：2021-01-06 00:00:00
abstract：:The distribution of the molluscan cardioexcitatory tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2) in the brain of the cloudy dogfish, Scyliorhinus torazame, was examined by immunocytochemistry. FMRFamide-like immunoreactivity was demonstrated to occur extensively in various regions of the dogfish brain, except for the c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00398072
更新日期：1991-08-01 00:00:00
abstract：:Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051032
更新日期：1998-04-01 00:00:00
abstract：:As luminal fluid moves from the seminiferous tubule and enters the rete testis, its protein concentration declines from approximately 6 mg/ml to 1 mg/ml. It was therefore suggested that protein is either 1) utilized by the spermatozoa, 2) transported across the epithelium of the terminal segment of the seminiferous tu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213810
更新日期：1983-01-01 00:00:00
abstract：:Three types of sensilla occurring on the lips and on the antennae of Peripatopsis moseleyi have been investigated by scanning and transmission electron microscopy. On the lips sensory spines can be found which contain numerous cilia originating from bipolar receptor cells. They reach the tip of the spine where the cut...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220613
更新日期：1977-02-14 00:00:00
