Cellular localization of cystic fibrosis transmembrane regulator protein in piglet and mouse intestine.


:Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR antibodies. The labeling pattern was abolished by preincubation of anti-CFTR antibodies with the immunogen or when non-immune IgG was used in place of anti-CFTR antibodies. These results support other studies that suggest that alterations in goblet cell function may be involved in the intestinal abnormalities associated with cystic fibrosis.


Cell Tissue Res


Cell and tissue research


Hayden UL,Carey HV




Has Abstract


1996-02-01 00:00:00












  • GDNF synthesis, signaling, and retrograde transport in motor neurons.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. Since its discover...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Cintrón-Colón AF,Almeida-Alves G,Boynton AM,Spitsbergen JM

    更新日期:2020-10-01 00:00:00

  • Somatotrophs and lactotrophs in the anterior pituitary of fetal and neonatal rats. Electron-microscopic immunocytochemical identification.

    abstract::The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particul...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hemming FJ,Dubois MP,Dubois PM

    更新日期:1986-01-01 00:00:00

  • The adenohypophysis of the flounder, Pleuronectes flesus, and the minnow, Phoxinus phoxinus.

    abstract::The pituitary gland of the flounder, Pleuronectes flesus, showed several unusual cytological features. Between the RPD and the PPD was a zone of cells that stained purple with Alcian blue--PAS--orange G. Many of these cells were apparently degenerating. In the PPD the strands and coils of presumptive STH cells showed ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Benjamin M

    更新日期:1975-01-01 00:00:00

  • Isotocinergic neurons in the goldfish hypothalamus: physiological and morphological studies on chemically identified cells.

    abstract::Isotocinergic (IT) neurons show physiological and morphological characteristcs that are similar to those of the other preoptic neuroendocrine cells in the goldfish. Preoptic IT cells show resting membrane potentials of 20-55 mV, action potentials of up to 100 mV, and physiological evidence of axonal branching. Dye-mar...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Reaves TA Jr,Hayward JN

    更新日期:1979-10-02 00:00:00

  • The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions.

    abstract::N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pollerberg GE,Burridge K,Krebs KE,Goodman SR,Schachner M

    更新日期:1987-10-01 00:00:00

  • Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine.

    abstract::There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fakhry J,Wang J,Martins P,Fothergill LJ,Hunne B,Prieur P,Shulkes A,Rehfeld JF,Callaghan B,Furness JB

    更新日期:2017-08-01 00:00:00

  • Ecdysteroid binding sites localized by autoradiography in the central nervous system of precommitment fifth-stadium Manduca sexta larvae.

    abstract::Brains and subesophageal ganglia from day 3.5 fifth stadium larvae of Manduca sexta were incubated in vitro with 4 nM tritiated ponasterone A, a 20-hydroxyecdysone analog, to determine whether uptake and specific binding of ecdysteroids occur at a cellular level. These tissues, which were taken just prior to the commi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bidmon HJ,Stumpf WE,Granger NA

    更新日期:1991-01-01 00:00:00

  • Rate of protein synthesis in rat salivary gland cells after pilocarpine or feeding. II. Protein synthesis in vivo in cells of the submandibular and parotid gland after secretion.

    abstract::The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kuijper-Lenstra AH,Kramer MF,van Venrooij WJ

    更新日期:1975-12-18 00:00:00

  • P2X(2) purine receptor immunoreactivity of intraganglionic laminar endings in the mouse gastrointestinal tract.

    abstract::The distribution of P2X(2) purine receptor subunit immunoreactivity has been investigated in the mouse gastrointestinal tract. Immunoreactivity occurred in intraganglionic laminar endings (IGLEs) associated with myenteric ganglia throughout the gastrointestinal tract. In the esophagus, IGLEs supplied every myenteric g...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Castelucci P,Robbins HL,Furness JB

    更新日期:2003-05-01 00:00:00

  • Efferent neurons of the lateral-line system and the VIII cranial nerve in the brainstem of anurans. A comparative study using retrograde tracer methods.

    abstract::The octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. This system is organized similarly in all larval anurans and in all adult aglossids. All have two groups of efferent neurons in the nucleus reticularis medialis between the VIIIth and th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Will U

    更新日期:1982-01-01 00:00:00

  • The pigmentary system of planaria. II. Physiology and functional morphology.

    abstract::The pigmentary system of the planaria, Dugesia gonocephala s.l. (Platyhelminthes, Turbellaria, Tricladida), consists of granules contained in chromatophore cells distributed in the parenchyma tissue. The administration of MSH release-inhibiting Factor (M.I.F.) leads to an easily observable general decolouration of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Palladini G,Medolago-Albani L,Margotta V,Conforti A,Carolei A

    更新日期:1979-06-27 00:00:00

  • Immunohistochemical study on the development of CRF-containing neurons in the hypothalamus of the rat.

    abstract::Appearance of immunoreactive corticotropin-releasing factor (CRF)-containing neurons was studied in developing hypothalamus of the rat by use of antisera against rat- and ovine CRF. These neurons were first recognized in the lateral and paraventricular nuclei on days 15.5 and 16.5 of gestation, respectively, when anti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Daikoku S,Okamura Y,Kawano H,Tsuruo Y,Maegawa M,Shibasaki T

    更新日期:1984-01-01 00:00:00

  • The metathoracic wing-hinge chordotonal organ of an atympanate moth, Actias luna (Lepidoptera, Saturniidae): a light- and electron-microscopic study.

    abstract::The structure of a simple chordotonal organ, the presumed homologue of the noctuoid moth tympanal organ, is described in the atympanate moth, Actias luna. The organ consists of a proximal scolopidial region and a distal strand, which attaches peripherally to the membraneous cuticle ventral to the hindwing alula. The s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yack JE,Roots BI

    更新日期:1992-03-01 00:00:00

  • Colocalization of NADPH-diaphorase activity and certain neuropeptides in the esophagus of opossum (Didelphis virginiana).

    abstract::Nitric oxide and various neuropeptides in the myenteric plexus regulate esophageal motility. We sought colocalization of nitric oxide synthase and neuropeptides in frozen sections of mid-portion of smooth-muscled opossum esophagus using NADPH-diaphorase activity to mark the synthase and immunoreactivity to detect pept...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Christensen J,Fang S

    更新日期:1994-12-01 00:00:00

  • Effects of colchicine on the ultrastructure of mouse taste buds.

    abstract::Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Takeda M,Suzuki Y,Shishido Y

    更新日期:1985-01-01 00:00:00

  • Components and connections of the circadian timing system in mammals.

    abstract::The circadian timing system is a neural network consisting of the hypothalamic suprachiasmatic nucleus, aided by the retina, other hypothalamic nuclei, autonomic regions of the spinal cord, sympathetic ganglia and the pineal gland. Extensive studies conducted over the last two decades have unravelled the principal ite...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Reuss S

    更新日期:1996-09-01 00:00:00

  • Immunohistochemical localization of urotensin I and other neuropeptides in the caudal neurosecretory system of three species of teleosts and two species of elasmobranchs.

    abstract::In three species of teleosts - carp Cyprinus carpio; grass carp Ctenopharyngodon idella; and crucian carp Carassius auratus - the caudal neurosecretory system displays small, medium-sized and large neurons. Urotensin I (UI)-immunoreactive and UI-nonreactive neurons were found in all three groups; in general, the numbe...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yamada C,Yamada S,Ichikawa T,Kobayashi H

    更新日期:1986-01-01 00:00:00

  • Scanning electron-microscope observations of the perikaryal projections of rabbit spinal ganglion neurons after enzymatic removal of connective tissue and satellite cells.

    abstract::The true surface of rabbit spinal ganglion neurons has been made directly accessible to scanning electron-microscope observation after removal of both the connective tissue and satellite cells that normally cover it. The neuronal surface is characterized by a profusion of slender projections whose shapes have been det...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pannese E,Ledda M,Conte V,Procacci P,Matsuda S

    更新日期:1990-04-01 00:00:00

  • Specialized contacts between sarcolemma and sarcoplasmic reticulum at the ends of muscle fibers in the diaphragm of the rat.

    abstract::An electron-microscopic study of the myotendinous portion of the diaphragm in the Wistar rat has shown that at the ends of muscle fibers, longitudinally oriented invaginations and peripheral furrows of the sarcolemma establish specialized contacts with individual sacs of the sarcoplasmic reticulum. The construction of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Andreev DP,Wassilev WA

    更新日期:1986-01-01 00:00:00

  • The Ca(2+)-binding protein calretinin is selectively enriched in a subpopulation of the epithelial rests of Malassez.

    abstract::During tooth development, the inner and outer enamel epithelia fuse by mitotic activity to produce a bilayered epithelial sheath termed Hertwig's epithelial root sheath (HERS). The epithelial rests of Malassez (ERM) are the developmental residues of HERS and remain in the adult periodontal ligament (PDL). Although the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Korkmaz Y,Klinz FJ,Beikler T,Blauhut T,Schneider K,Addicks K,Bloch W,Raab WH

    更新日期:2010-12-01 00:00:00

  • Analysis of the expression of p53 during the morphogenesis of the gastroesophageal mucosa of Gallus gallus domesticus (Linnaeus, 1758).

    abstract::Ontogenesis comprises a series of events including cell proliferation and apoptosis and resulting in the normal development of the embryo. Protein p53 has been described as being involved in the development of several animal species. The aim of this study was to analyze the expression of protein p53 during the morphog...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ventura A,do Nascimento AA,dos Santos MA,Vieira-Lopes DA,Sales A,Pinheiro NL

    更新日期:2014-01-01 00:00:00

  • Electron-microscopic studies on the presence of gap junctions between corneal fibroblasts in rabbits.

    abstract::Corneal fibroblasts, major cellular components of the corneal stroma, are loosely arrayed between collagen lamellae. They play an important role in the metabolic and physiological homeostasis mechanisms by which the cornea is kept transparent. This paper deals with the demonstration of the gap junctions between the co...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ueda A,Nishida T,Otori T,Fujita H

    更新日期:1987-08-01 00:00:00

  • Coding of pheromones by vomeronasal receptors.

    abstract::Communication between individuals is critical for species survival, reproduction, and expansion. Most terrestrial species, with the exception of humans who predominantly use vision and phonation to create their social network, rely on the detection and decoding of olfactory signals, which are widely known as pheromone...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Tirindelli R

    更新日期:2021-01-12 00:00:00

  • Embryonic chicken gizzard: smooth muscle and non-muscle myosin isoforms.

    abstract::Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independen...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Paul ER,Christian AL,Franke R,Gröschel-Stewart U

    更新日期:1994-05-01 00:00:00

  • Ultrastructural changes in the spiral limbus associated with carboplatin-induced ablation of inner hair cells.

    abstract::Four months after the selective ablation of inner hair cells by carboplatin, the interdental cell epithelium exhibited dilated intercellular spaces and cytosolic vacuoles not seen in controls. In addition, the wide, often electron-lucent phalanges observed in the interdental cells of the normal chinchilla collapsed in...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Spicer SS,Salvi RJ,Schulte BA

    更新日期:2000-10-01 00:00:00

  • Ultrastructural evidence of gonadotrophin release from castration cells following injection of LHRH in the rat.

    abstract::To examine the morphological evidence of the nature of hormone release from castration cells the author gonadectomized neonatal male and female rats. Twenty-eight days after the operation the animals were pretreated with estrogen, progesterone, or estrogen and progesterone in combination, every other day for one week,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Shiino M

    更新日期:1982-01-01 00:00:00

  • Developmental patterns of proteoglycan-containing extracellular matrix in perineuronal nets and neuropil of the postnatal rat brain.

    abstract::The extracellular matrix is involved in various morphogenetic processes which are accompanied by changes in its physicochemical properties and spatial organization. In the adult brain it contributes to cellular communication and the regulation of neuronal activity. The present study deals with the postnatal appearance...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Köppe G,Brückner G,Brauer K,Härtig W,Bigl V

    更新日期:1997-04-01 00:00:00

  • Quantitative analysis of denatured collagen by collagenase digestion and subsequent MALDI-TOF mass spectrometry.

    abstract::Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Nimptsch A,Schibur S,Ihling C,Sinz A,Riemer T,Huster D,Schiller J

    更新日期:2011-03-01 00:00:00

  • Plasticity and regulatory mechanisms of Hox gene expression in mouse neural crest cells.

    abstract::In amniotes, the developmental potentials of neural crest cells differ between the cranium and the trunk. These differences may be attributable to the different expression patterns of Hox genes between cranial and trunk neural crest cells. However, little is known about the factors that control Hox genes expression in...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ishikawa S,Ito K

    更新日期:2009-09-01 00:00:00

  • Localization of juxtacrine factor ephrin-B2 in pituitary stem/progenitor cell niches throughout life.

    abstract::We have recently reported that Sox2-expressing pituitary stem/progenitor cells contact each other via a tight-junction protein CAR to form stem/progenitor cell niches in the marginal cell layer facing the lumen and in the clusters scattered in the parenchyma of the anterior lobe. However, the microenvironment of the n...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yoshida S,Kato T,Higuchi M,Chen M,Ueharu H,Nishimura N,Kato Y

    更新日期:2015-03-01 00:00:00