Abstract：
:The protein synthesis inhibitor cycloheximide (Chx) suppresses prolactin-induced beta-casein gene expression in the mammary epithelial cell line COMMA-D. As the mechanism underlying this effect is unclear, the effects of protein synthesis inhibitors on interactions of transcription factors with the beta-casein promoter were examined. Suppression of prolactin-induced beta-casein gene expression occurred in both COMMA-D cells and primary mammary cell cultures with as little as 2 h protein synthesis inhibition. This was associated with changes in transcription factors interacting at a response element in the proximal region of the rat beta-casein promoter. Inhibition of protein synthesis was associated with NF-kappaB binding at a site immediately 3' to the Stat5-binding site at position 97-89 of the beta-casein promoter, suppression of Stat5 DNA-binding activity, and inhibition of Stat5 tyrosine phosphorylation. Treatment with the NF-kappaB inhibitor parthenolide failed to restore prolactin responsiveness. These results show that protein synthesis inhibition is associated with both blockage of prolactin-Stat5 signaling and NF-kappaB binding to the beta-casein promoter, but that the latter is not necessary for the suppression of beta-casein expression.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Beaton A,Broadhurst MK,Wilkins RJ,Wheeler TTdoi
10.1007/s00441-002-0672-2keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
207-15issue
2eissn
0302-766Xissn
1432-0878journal_volume
311pub_type
杂志文章abstract：:The distribution of neuropeptide Y (NPY)-like immunoreactivity in the brain of the bichir, Polypterus senegalus, was examined immunohistochemically. NPY-like immunoreactivity was distributed widely in the brain, with the highest density in the diencephalon. NPY-positive perikarya were found in various areas, including...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050874
更新日期：1997-08-01 00:00:00
abstract：:The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various ma...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0666-9
更新日期：2008-10-01 00:00:00
abstract：:Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050641
更新日期：1996-08-01 00:00:00
abstract：:The ontogeny of protein gene product 9.5 (PGP 9.5), serotonin (5-HT), calcitonin gene-related peptide (CGRP), and calcitonin (CT) immunoreactivity was evaluated in small-granule endocrine cells of hamster laryngotracheal epithelium from fetal day 11 to adulthood. Two centrifugal (proximal-to-distal) patterns of differ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305382
更新日期：1994-01-01 00:00:00
abstract：:The ultrastructure and distribution of adherens junctions in the intact adult lens of human, chicken, dove, rat, and rainbow trout were studied with thin-section electron microscopy, using an improved fixation containing a mixture of glutaraldehyde, lysine, and tannic acid. The nature of adherens junctions in the fibe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220014
更新日期：1988-10-01 00:00:00
abstract：:A body of evidence points to the existence of stem cell stores in adult tissues, in addition to the well-known hematopoietic stem cells from bone marrow. Many reports describe the ability of these multipotent cells (developmentally non-compromised with their organs of origin) to give rise to many different cell types ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-006-0172-x
更新日期：2006-09-01 00:00:00
abstract：:Stem cells are an attractive cellular source for regenerative medicine and tissue engineering applications due to their multipotency. Although the elasticity of the extracellular matrix (ECM) has been shown to have crucial impacts in directing stem cell differentiation, it is not the only contributing factor. Many res...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-015-2190-z
更新日期：2015-09-01 00:00:00
abstract：:NdWFamide is an Aplysia cardioexcitatory tri-peptide containing D-tryptophan. To investigate the roles of this peptide, we examined the immunohistochemical distribution of NdWFamide-positive neurons in Aplysia tissues. All the ganglia of the central nervous system (CNS) contained NdWFamide-positive neurons. In particu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0707-3
更新日期：2003-04-01 00:00:00
abstract：:To investigate the role of RhoA on the intracellular membrane dynamics of lysosomes in rat hepatoma cells (MM1), we analyzed the localization of lysosomal aspartic proteinase cathepsin D by confocal immunofluorescence microscopy in the dominant active RhoA-transfected cells. Here we show that the transfection of the d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:Hepatic peroxisomes in human embryos with a menstrual age of 6 and 7 weeks have been examined via catalase cytochemistry. In the younger sample, the organelles show no catalase activity, their matrix being pale and coarsely reticular. In the 7-week specimen, the peroxisome population consists of catalase-positive and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00323574
更新日期：1993-04-01 00:00:00
abstract：:In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of the major Cx32 protein is downregulated during liver regeneration and cholestasis. Here we have analyzed the acute-phase response (after experimental inflammation) an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000177
更新日期：2000-04-01 00:00:00
abstract：:The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218017
更新日期：1985-01-01 00:00:00
abstract：:Fibroblast growth factors (Fgfs) are proteins with diverse functions in development, repair, and metabolism. The human Fgf gene family with 22 members can be classified into three groups, canonical, intracellular, and hormone-like Fgf genes. In contrast to canonical and intracellular Fgfs identified in invertebrates a...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1024-2
更新日期：2010-10-01 00:00:00
abstract：:Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318364
更新日期：1995-06-01 00:00:00
abstract：:Considerable evidence indicates that the state of ocular connective tissues and their response in glaucomatous disease affect the degree of glaucoma damage. Both experimental and clinical data suggest that improved diagnostic and prognostic information can be derived from the assessment of the mechanical responsivenes...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1603-0
更新日期：2013-08-01 00:00:00
abstract：:Chorionic binucleate cells (BNC) occur in several ruminants including cow, deer, goat and sheep. They migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. We have compared the BNC of near-term, resin-embedded, ovine and bovine ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331380
更新日期：1994-12-01 00:00:00
abstract：:Rat thymic phagocytic cells were characterized in vitro using various light- and electron-microscopical techniques. Thymic cell suspensions were mechanically prepared and enriched for non-lymphoid cells, which were predominantly phagocytic and of three types. Type I showed acid phosphatase (APh) activity in small gran...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222183
更新日期：1983-01-01 00:00:00
abstract：:Bullous pemphigoid antigen-1 (BPA1) and alpha(6)beta(4)-integrin colocalize at the hemidesmosomes in basal-layer keratinocytes of normal squamous epithelia. The expression of these genes was analyzed during the process of tumor cell invasion and metastasis on frozen sections of head and neck biopsies, and the structur...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100462
更新日期：2001-12-01 00:00:00
abstract：:The development of the heterophil granulocyte in the bone marrow of the rat is described, and an electron-microscopical analysis of the changes in the cytoplasm as well as in the granule population in several stages of maturation is reported. Three types of granule originate in consecutive stages of heterophil maturat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00211466
更新日期：1983-01-01 00:00:00
abstract：:Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2597-9
更新日期：2017-08-01 00:00:00
abstract：:Peeling skin syndrome is a heterogeneous group of rare disorders. Peeling skin, leukonychia, acral punctate keratoses, cheilitis and knuckle pads (PLACK syndrome, OMIM616295) is a newly described form of PSS with an autosomal recessive mode of inheritance. We report a 5.5-year-old boy with features of PLACK syndrome. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03077-9
更新日期：2019-11-01 00:00:00
abstract：:The general ultrastructure of the electrocyte, the basic unit of the electric organs of Electrophorus electricus, is analyzed. Presented herein are detailed observations of the syncytial surface, its fibrillar coat, invaginations of the plasma membrane and synaptic terminals. Using Thiéry's method glycogen granules we...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220681
更新日期：1976-11-10 00:00:00
abstract：:Using techniques for enhanced microtubular preservation (albumin pretreatment of Gray) occipital and pyriform cortices of rats were studied by electron microscopy at various stages of development. A close structural relationship was seen between microtubules (mts), focal membrane densities, synaptic vesicles, and pres...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215777
更新日期：1983-01-01 00:00:00
abstract：:In vitro incubation of autonomic nerves with vinblastine sulphate (1 times 10(-4) M) caused a disappearance of microtubules within 15 min; during the following 15 min paracrystalline arrays appeared within the axons. An increase in the abundance of microfilaments was also observed, but these did not appear to arise fr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220136
更新日期：1976-02-25 00:00:00
abstract：:The mole has a single pair of accessory sex organs with features of both the prostate and the seminal vesicle, for which the term prostate gland is not appropriate. Seasonal changes occuring in this gland were related to four periods: a) the quiescence period, b) the maturation period, c) the active period and d) the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218718
更新日期：1976-12-10 00:00:00
abstract：:In the rostral hypothalamus of the domestic fowl, the magnocellular neurosecretory nuclei show a peculiar differentiation. Golgi studies of the supraoptic and paraventricular nuclei of the fowl reveal at least two major cell types: 1) large multipolar neurons, and 2) small interneurons. Golgi impregnations provide a d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236253
更新日期：1981-01-01 00:00:00
abstract：:The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223268
更新日期：1975-09-16 00:00:00
abstract：:Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1839-3
更新日期：2014-07-01 00:00:00
abstract：:The distribution of the molluscan cardioexcitatory tetrapeptide FMRFamide (Phe-Met-Arg-Phe-NH2) in the brain of the cloudy dogfish, Scyliorhinus torazame, was examined by immunocytochemistry. FMRFamide-like immunoreactivity was demonstrated to occur extensively in various regions of the dogfish brain, except for the c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00398072
更新日期：1991-08-01 00:00:00
abstract：:An electron microscopical investigation of the pineal gland of the adult noctule bat revealed the presence of some peculiar ciliary derivatives, similar to the club-shaped outer segment of rudimentary photoreceptor cells in the pineal organ of nonmammalian vertebrates. The pinealocytes of population I can be classifie...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222058
更新日期：1977-07-26 00:00:00
