Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli.

abstract：:The regulation of the photosynthetic genes by DNA supercoiling in Rhodobacter capsulatus has been studied by using gyrase inhibitors in vivo and by measurement of mRNA levels of more than a dozen genes. The results demonstrate that the levels of mRNA for light-harvesting (I, II) and reaction center (L, M, H) proteins,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu YS,Hearst JE

更新日期：1988-06-01 00:00:00

abstract：:Opportunistic infections are often polymicrobial. Two of the most important bacterial opportunistic pathogens of humans, Pseudomonas aeruginosa and Staphylococcus aureus, frequently are coisolated from infections of catheters, endotracheal tubes, skin, eyes, and the respiratory tract, including the airways of people w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman LR,Déziel E,D'Argenio DA,Lépine F,Emerson J,McNamara S,Gibson RL,Ramsey BW,Miller SI

更新日期：2006-12-26 00:00:00

abstract：:Three naturally occurring dermorphin-like peptides from the skin of the frog Phyllomedusa bicolor, the related carboxyl-terminal amides, and some substituted analogs were synthesized, their binding profiles to opioid receptors were determined, and their biological activities were studied in isolated organ preparations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Negri L,Erspamer GF,Severini C,Potenza RL,Melchiorri P,Erspamer V

更新日期：1992-08-01 00:00:00

abstract：:Historical collapse of ancient states or civilizations has raised new awareness about its possible relevance to current issues of sustainability, in the context of global change. This Special Feature examines 12 case studies of societies under stress, of which seven suffered severe transformation. Outcomes were comple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butzer KW,Endfield GH

更新日期：2012-03-06 00:00:00

abstract：:The hypothesis that perceived pain intensity can influence placebo analgesia was tested. One hundred and seven subjects rated their pain from from 0 to 10 on a visual analog scale after a standard wisdom tooth extraction. The expected course of such postoperative pain in the absence of therapy or placebo is a steady i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine JD,Gordon NC,Bornstein JC,Fields HL

更新日期：1979-07-01 00:00:00

abstract：:Factors that elevate amyloid-beta (Abeta) peptide levels are associated with an increased risk for Alzheimer's disease. Insulysin has been identified as one of several proteases potentially involved in Abeta degradation based on its hydrolysis of Abeta peptides in vitro. In this study, in vivo levels of brain Abeta40 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller BC,Eckman EA,Sambamurti K,Dobbs N,Chow KM,Eckman CB,Hersh LB,Thiele DL

更新日期：2003-05-13 00:00:00

abstract：:Monoclonal antibodies were used to isolate nonneutralizable antigenic variants in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. It had been found that a large percentage of virus retains infectivity despite binding neutralizing antibody. This high persistent fraction of nonneutralized virus ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iorio RM,Bratt MA

更新日期：1985-10-01 00:00:00

abstract：:Vasopressin's action in renal cells to regulate water transport depends on protein phosphorylation. Here we used mass spectrometry-based quantitative phosphoproteomics to identify signaling pathways involved in the short-term V2-receptor-mediated response in cultured collecting duct cells (mpkCCD) from mouse. Using St...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rinschen MM,Yu MJ,Wang G,Boja ES,Hoffert JD,Pisitkun T,Knepper MA

更新日期：2010-02-23 00:00:00

abstract：:Dietary restriction has been shown to have several health benefits including increased insulin sensitivity, stress resistance, reduced morbidity, and increased life span. The mechanism remains unknown, but the need for a long-term reduction in caloric intake to achieve these benefits has been assumed. We report that w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anson RM,Guo Z,de Cabo R,Iyun T,Rios M,Hagepanos A,Ingram DK,Lane MA,Mattson MP

更新日期：2003-05-13 00:00:00

abstract：:Bacteria use membrane-integral sensor histidine kinases (HK) to perceive stimuli and transduce signals from the environment to the cytosol. Information on how the signal is transmitted across the membrane by HKs is still scarce. Combining both liquid- and solid-state NMR, we demonstrate that structural rearrangements ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salvi M,Schomburg B,Giller K,Graf S,Unden G,Becker S,Lange A,Griesinger C

更新日期：2017-03-21 00:00:00

abstract：:For isolated rat hepatocytes, glucagon, 3':5'-cyclic AMP, 3':5'-cyclic GMP, and epinephrine stimulate the rate of gluconeogenesis from substrates not involving pathways of mitochondrial metabolism. From estimation of the rates of glucose formation, fructose 6-phosphate phosphorylation, and lactate and pyruvate formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kneer NM,Bosch AL,Clark MG,Lardy HA

更新日期：1974-11-01 00:00:00

abstract：:Population expansion in space, or range expansion, is widespread in nature and in clinical settings. Space competition among heterogeneous subpopulations during range expansion is essential to population ecology, and it may involve the interplay of multiple factors, primarily growth and motility of individuals. Struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuo W,Wu Y

更新日期：2020-03-03 00:00:00

abstract：:MicroRNAs (miRNAs) are small 19- to 24-nt noncoding RNAs that have the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In cancer, miRNAs may function as oncogenes or tumor suppressors. Here, we conducted global profiling for miRNAs in a cohort of stage 1 nonsmall ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng Y,Dai Y,Hitchcock C,Yang X,Kassis ES,Liu L,Luo Z,Sun HL,Cui R,Wei H,Kim T,Lee TJ,Jeon YJ,Nuovo GJ,Volinia S,He Q,Yu J,Nana-Sinkam P,Croce CM

更新日期：2013-09-10 00:00:00

abstract：:Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henning U,Rehn K,Hoehn B

更新日期：1973-07-01 00:00:00

abstract：:The Pleistocene global dispersal of modern humans required the transit of arid and semiarid regions where the distribution of potable water provided a primary constraint on dispersal pathways. Here, we provide a spatially explicit continental-scale assessment of the opportunities for Pleistocene human occupation of Au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bird MI,O'Grady D,Ulm S

更新日期：2016-10-11 00:00:00

abstract：:The hippocampus and cerebellum are critically involved in trace eyeblink classical conditioning (EBCC). The mechanisms underlying the hippocampal-cerebellar interaction during this task are not well-understood, although hippocampal theta (3-7 Hz) oscillations are known to reflect a favorable state for EBCC. Two groups...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffmann LC,Berry SD

更新日期：2009-12-15 00:00:00

abstract：:A sensitive, labor-saving, and easily automatable nonradioactive procedure named APEX-FCS (amplified probe extension detected by fluorescence correlation spectroscopy) has been established to detect specific in vitro amplification of pathogen genomic sequences. As an example, Mycobacterium tuberculosis genomic DNA was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter NG,Schwille P,Eigen M

更新日期：1996-11-12 00:00:00

abstract：:Antigen.antibody complexes (Ag.Ab) prepared from (125)I-labeled bovine serum albumin and guinea pig anti-albumin were incubated at 37 degrees C for 30 min with normal human serum diluted optimally for binding (1:16) and then with autologous erythrocytes (RBC). After washing, RBC-bearing antigen.antibody.complement com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medof ME,Prince GM,Mold C

更新日期：1982-08-01 00:00:00

abstract：:The protease encoded by the human immunodeficiency virus (HIV) processes the viral gag and gag-pol protein precursor by posttranslational cleavage. In this study we have demonstrated by site-specific mutagenesis (Asp----Thr) and by pepstatin A inhibition that the recombinant HIV protease is an aspartic-type protease. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seelmeier S,Schmidt H,Turk V,von der Helm K

更新日期：1988-09-01 00:00:00

abstract：:A full-length cDNA clone encoding a purified augmenter of liver regeneration (ALR) factor prepared from the cytosol of weanling rat livers was isolated. The 1.2-kb cDNA included a 299-bp 5' untranslated region, a 375-bp coding region, and a 550-bp 3' untranslated region. It encoded a protein consisting of 125 amino ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hagiya M,Francavilla A,Polimeno L,Ihara I,Sakai H,Seki T,Shimonishi M,Porter KA,Starzl TE

更新日期：1994-08-16 00:00:00

abstract：:Exposure of murine hepatoma (Hepa 1c1c7) cells to a variety of chemical agents known to protect animals against the neoplastic, mutagenic, and other toxic effects of chemical carcinogens results in dose- and time-dependent inductions of NAD(P)H:quinone reductase (EC 1.6.99.2). This enzyme protects against quinone toxi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Long MJ,Prochaska HJ,Talalay P

更新日期：1986-02-01 00:00:00

abstract：:Myosin V, a double-headed molecular motor, transports organelles within cells by walking processively along actin, a process that requires coordination between the heads. To understand the mechanism underlying this coordination, processive runs of single myosin V molecules were perturbed by varying nucleotide content....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baker JE,Krementsova EB,Kennedy GG,Armstrong A,Trybus KM,Warshaw DM

更新日期：2004-04-13 00:00:00

abstract：:A pluripotent cell line, C3H10T1/2, is induced to undergo adipogenesis by a mixture of factors that includes a glucocorticoid such as dexamethasone. We found that expression of myostatin (MSTN), a TGF-beta family member extensively studied in muscle, was induced by dexamethasone under those differentiation conditions....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman BJ,Streeper RS,Farese RV Jr,Yamamoto KR

更新日期：2006-10-17 00:00:00

abstract：:Recent analysis of complete eukaryotic genome sequences has revealed that gene duplication has been rampant. Moreover, next to a continuous mode of gene duplication, in many eukaryotic organisms the complete genome has been duplicated in their evolutionary past. Such large-scale gene duplication events have been assoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maere S,De Bodt S,Raes J,Casneuf T,Van Montagu M,Kuiper M,Van de Peer Y

更新日期：2005-04-12 00:00:00

abstract：:Human malignant pleural mesothelioma (MPM) is considered a rare tumor, but recent estimations indicate that one-quarter million people will die of this neoplasm in Europe in the next three decades. The mineral asbestos is considered the main causative agent of this neoplasm. MPM is largely unresponsive to conventional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazzoni E,Corallini A,Cristaudo A,Taronna A,Tassi G,Manfrini M,Comar M,Bovenzi M,Guaschino R,Vaniglia F,Magnani C,Casali F,Rezza G,Barbanti-Brodano G,Martini F,Tognon MG

更新日期：2012-10-30 00:00:00

abstract：:Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered energy homeostasis, we have generated mice lacking all POMC-derived peptides. Consistent with a previously reported mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challis BG,Coll AP,Yeo GS,Pinnock SB,Dickson SL,Thresher RR,Dixon J,Zahn D,Rochford JJ,White A,Oliver RL,Millington G,Aparicio SA,Colledge WH,Russ AP,Carlton MB,O'Rahilly S

更新日期：2004-03-30 00:00:00

abstract：:We investigate the roles of the pseudospin and the valley degeneracy in electron scattering at graphene edges. It is found that they are strongly correlated with charge density modulations of short-wavelength oscillations and slowly decaying beat patterns in the electronic density profile. Theoretical analyses using n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park C,Yang H,Mayne AJ,Dujardin G,Seo S,Kuk Y,Ihm J,Kim G

更新日期：2011-11-15 00:00:00

abstract：:In Arabidopsis thaliana, biosynthesis of the essential thiol antioxidant, glutathione (GSH), is plastid-regulated, but many GSH functions, including heavy metal detoxification and plant defense activation, depend on cytosolic GSH. This finding suggests that plastid and cytosol thiol pools are closely integrated and we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maughan SC,Pasternak M,Cairns N,Kiddle G,Brach T,Jarvis R,Haas F,Nieuwland J,Lim B,Müller C,Salcedo-Sora E,Kruse C,Orsel M,Hell R,Miller AJ,Bray P,Foyer CH,Murray JA,Meyer AJ,Cobbett CS

更新日期：2010-02-02 00:00:00

abstract：:Phagocytic cells are a critical line of defense against infection. The ability of a pathogen to survive and even replicate within phagocytic cells is a potent method of evading the defense mechanisms of the host. A number of pathogens survive within macrophages after phagocytosis and this contributes to their virulenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindgren SW,Stojiljkovic I,Heffron F

更新日期：1996-04-30 00:00:00

abstract：:Coordinated near-threshold depolarized states in cortical and striatal neurons may contribute to form functionally segregated channels of information processing. Recent anatomical studies have identified pathways that could support spiraling interactions across corticostriatal channels, but a functional outcome of suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kasanetz F,Riquelme LA,Della-Maggiore V,O'Donnell P,Murer MG

更新日期：2008-06-10 00:00:00