Abstract:
:Highly purified and metabolically viable gamma-aminobutyric acid (GABA)ergic and cholinergic synaptosomes were prepared from mammalian brain using antisera raised against glutamate decarboxylase and choline acetyltransferase respectively. These antisera appear to recognise specific outwardly facing components of GABAergic and cholinergic synaptic plasma membranes, as well as the specific neurotransmitter-synthesizing enzymes contained within the particular nerve terminal. After cell surface labelling had occurred, synaptosomes were incubated with magnetic microspheres which had previously been coupled with protein A. Labelled synaptosomes were then retrievable in a magnetic field. This separation technique allowed mg quantities of purified-synaptosomes to be prepared which contained less than 2% non-specific contaminants.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Docherty M,Bradford HF,Wu JYdoi
10.1016/0304-3940(87)91004-4subject
Has Abstractpub_date
1987-10-16 00:00:00pages
232-8issue
1-2eissn
0304-3940issn
1872-7972pii
0304-3940(87)91004-4journal_volume
81pub_type
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