Studies on ABH antigen grouping of ammoniacal extracts of bloodstains by absorption-elution.

Abstract:

:The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclonal anti-A and anti-B and affinity-purified Ulex europaeus agglutinin I (UEA I) reagents were employed for comparison in these processes. Ammoniacal extracts of bloodstains and dried bloodstains on cotton substrata behaved comparably with respect to the parameters studied. The monoclonal anti-A and anti-B and UEA I reagents studied yielded satisfactory results, comparable in some cases to conventional reagents, with respect to the parameters studied.

journal_name

Forensic Sci Int

authors

Gaensslen RE,Lee HC,DeGraw SS,Carroll-Reho J

doi

10.1016/0379-0738(86)90182-9

subject

Has Abstract

pub_date

1986-07-14 00:00:00

pages

145-57

issue

3

eissn

0379-0738

issn

1872-6283

pii

0379-0738(86)90182-9

journal_volume

31

pub_type

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