Analysis of lysergic acid diethylamide: comparison of capillary electrophoresis with laser-induced fluorescence (CE-LIF) with conventional techniques.

Abstract:

:The increasing use of the potent hallucinogenic drug D-lysergic acid diethylamide (LSD) makes the application of highly sensitive analytical techniques necessary. For an effective analysis of biological specimens a limit of detection in the sub-ng/ml range is required. Using capillary electrophoresis (CE), with laser-induced fluorescence detection it was possible to determine 0.1-0.2 ng LSD/ml blood. The conventionally used immunoassays and gas and high-performance liquid chromatographic methods have cut-off values and limit of detection, respectively, in the same range. Only tandem mass spectrometry techniques can be more sensitive. Therefore, CE can be considered a good complementary method to conventional immunological and chromatographic techniques for the forensic analysis of biofluids.

journal_name

Forensic Sci Int

authors

Frost M,Köhler H

doi

10.1016/s0379-0738(98)00017-6

subject

Has Abstract

pub_date

1998-04-05 00:00:00

pages

213-8

issue

2-3

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(98)00017-6

journal_volume

92

pub_type

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