Abstract：
:Due to the different types and quality of forensic evidence materials, their DNA content can vary substantially, and particularly low quantities can impact the results in an identification analysis. In this study, the quantity of mitochondrial and nuclear DNA was determined in a variety of materials using a previously described real-time PCR method. DNA quantification in the roots and distal sections of plucked and shed head hairs revealed large variations in DNA content particularly between the root and the shaft of plucked hairs. Also large intra- and inter-individual variations were found among hairs. In addition, DNA content was estimated in samples collected from fingerprints and accessories. The quantification of DNA on various items also displayed large variations, with some materials containing large amounts of nuclear DNA while no detectable nuclear DNA and only limited amounts of mitochondrial DNA were seen in others. Using this sensitive real-time PCR quantification assay, a better understanding was obtained regarding DNA content and variation in commonly analysed forensic evidence materials and this may guide the forensic scientist as to the best molecular biology approach for analysing various forensic evidence materials.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Andréasson H,Nilsson M,Budowle B,Lundberg H,Allen Mdoi
10.1016/j.forsciint.2005.11.024keywords:
subject
Has Abstractpub_date
2006-12-01 00:00:00pages
56-64issue
1eissn
0379-0738issn
1872-6283pii
S0379-0738(05)00625-0journal_volume
164pub_type
杂志文章abstract：:This is a preliminary report on significant alterations in the DNA profile caused by toxic substances which potentially has profound implications for the use of DNA techniques in identification. Acute DNA changes in the globus pallidus of the brain in man caused by carbon monoxide poisoning were detected by DNA profil...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(95)01879-4
更新日期：1996-04-23 00:00:00
abstract：:Little is currently known about the importance of clotting during the drying of blood pools. While this is of little moment for droplets where drying occurs faster than contact-phase-induced clotting, clotting may significantly influence blood pools drying as it transform a liquid into a gel. To investigate this influ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.110008
更新日期：2019-12-01 00:00:00
abstract：:A headspace solid-phase microextraction procedure (HS-SPME) was developed for the profiling of traces present in 3,4-methylenedioxymethylampethamine (MDMA). Traces were first extracted using HS-SPME and then analyzed by gas chromatography-mass spectroscopy (GC-MS). The HS-SPME conditions were optimized using varying c...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.03.004
更新日期：2009-05-30 00:00:00
abstract：:During a 3-year period (August 1st, 1985 to July 31st, 1988) a systematic investigation of medico-legal autopsy cases with regard to the presence of antibodies for HIV-virus was carried out at the Department of Forensic Medicine in Stockholm, Sweden. Prior to autopsy, blood samples were taken from femoral or subclavia...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(89)90220-x
更新日期：1989-06-01 00:00:00
abstract：:The location of a gunshot entrance wound as well as the wound path trajectory are the main findings that help to determine the position from which a weapon was fired and thus distinguish firearm suicides from homicides and accidents. We present a case of a 28-year old man, who was found dead in his car. Because of an ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.03.015
更新日期：2017-06-01 00:00:00
abstract：:Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00267-6
更新日期：2003-10-14 00:00:00
abstract：:Medicolegal malpractice assessments have been an important part of the work of forensic pathologists. Not only botchers are concerned but also physicians of all clinical subjects, especially malpractice charges claiming a malpractice leading to death. Single long articles and book chapters have been published about ma...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2004.04.063
更新日期：2004-09-10 00:00:00
abstract：:We examined histologically the meninges adherent to traumatic lesions of a patient with brain death sustained for 101 days and observed both early and late reactions of wound healing at the same site of the dura mater. Some parts of the dura mater were thick and histological examinations revealed formation of new vess...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(92)90178-y
更新日期：1992-10-01 00:00:00
abstract：:Urine morphine levels after the consumption of poppy seeds were measured in two separate trials. Maximum levels of approximately 18 micrograms/ml were found using RIA, EMIT-ST and GC methodologies. Positive immunoassay results were seen up to 60 h post-ingestion. Several different lots of seeds from various sources we...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(85)90173-2
更新日期：1985-02-01 00:00:00
abstract：:The chromium-doped zinc gallogermanate, Zn3Ga2Ge2O10:0.5%Cr3+, was prepared and modified for use as a near-infrared (NIR) luminescent fingerprint dusting powder. The optical properties of this material are illustrated and have been shown to offer versatility and promise in terms of overcoming troublesome backgrounds d...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.04.012
更新日期：2017-07-01 00:00:00
abstract：:Dental forensic identifications based on comparison of antemortem and postmortem radiographs provide effective and reliable evidence. There are no standardized procedures for assessing similarities between different types of dental radiographs (e.g. orthopantomograms, bitewings, and periapical radiographs), and the op...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.06.015
更新日期：2012-10-10 00:00:00
abstract：:An accurate and precise estimate of stature can be very useful in the analysis of human remains in forensic cases. A problem with many stature estimation methods is that an unknown individual must first be assigned to a specific group before a method can be applied. Group membership has been defined by sex, age, year ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.12.011
更新日期：2016-02-01 00:00:00
abstract：:The authors describe a technique for the gross examination of postmortem eyes of children who are suspected to have been the victims of deliberate trauma. By removing the anterior segment (cornea, iris, lens and pars plicata of the ciliary body) en bloc by a coronal incision through the pars plana just anterior to the...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(92)90149-q
更新日期：1992-09-01 00:00:00
abstract：:A total of 48 methamphetamine hydrochloride samples from eight seizures were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography with a flame ionization detector (GC-FID). Major impurities detected include 1,2-dimethyl-3-phenylaziridine, ephedrine/pseudoephedrine, 1,3-dimethyl-2-phenylna...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.09.012
更新日期：2008-11-20 00:00:00
abstract：INTRODUCTION:The intoxicated person may cause harm to others, often requiring expert evaluation for the determination of guilt. The primary aim of this study was to determine the mechanisms of mistakes that led 17 doctors accused of working under the influence of alcohol to face malpractice. We also wanted to clarify w...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.01.019
更新日期：2018-04-01 00:00:00
abstract：:In Dunedin (South Island, New Zealand), a sexual assault is reported to police approximately once every two to three weeks, with some reports fictitious. Identifying a fictitious claim is difficult, and damage to apparel, especially knickers, may be the only form of evidence. In this paper, the tear behaviour of three...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.10.029
更新日期：2012-04-10 00:00:00
abstract：:A rare case of death of a young man due to airway obstruction in the course of angioedema (Quincke's edema). Type I hereditary angioedema due to C1 esterase inhibitor deficiency had been diagnosed in the man while he was alive. The information concerning the man's health state was given in the Public Prosecutor's deci...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.07.025
更新日期：2015-09-01 00:00:00
abstract：:Buprenorphine, commonly known by the trademark Temgesic, is one of the most popular drugs of abuse among the opioid-addicted young individuals in Iran. Temgesic, Bungesic, etc. are the most popular and important illicit opioid drugs in Tehran's illicit drugs black market, and are now among the most widely abused by op...
journal_title：Forensic science international
pub_type： 信件
doi：10.1016/j.forsciint.2007.06.016
更新日期：2007-10-25 00:00:00
abstract：:Existing works on pellet dispersion ballistics are confined to some data-based models derived from statistical analysis of observed patterns on targets but the underlying process causing the dispersion lacks due attention. The present article delves into the relatively unexplored areas of dispersion phenomena, and att...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/0379-0738(92)90118-g
更新日期：1992-08-01 00:00:00
abstract：:One case of the erroneous administration of deslanoside and high level of drug in antemortem plasma and postmortem specimens has been reported owing to the unusual surrounding circumstances. Deslanoside in antemortem plasma was determined by FPIA and the analysis was done by HPLC in the postmortem tissue samples. The ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90179-3
更新日期：1990-04-01 00:00:00
abstract：:An interrelation between consumption of illegal drugs and committing an indictable offence has been repeatedly discussed in literature. In a retrospective study serum concentrations of illegal and legal drugs as well as data originating from police reports and examinations by physicians taking blood from individuals b...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.01.031
更新日期：2013-03-10 00:00:00
abstract：:The objective of this study was to evaluate the applicability of human identification parameters, established by Tatlisumak et al. (2007), by means of cone-beam computed tomography and extraoral radiographs of the frontal sinus region. From a total of 58 dry skulls, 26 were selected. Posteroanterior, profile cephalome...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.03.030
更新日期：2016-05-01 00:00:00
abstract：:Formalin-fixed and paraffin-embedded tissue (FF-PET) is an invaluable resource for retrospective molecular genetic studies, but the extraction of high-quality genomic DNA from FF-PET is still a problematic issue. Despite the range of DNA extraction methods currently in use, the association of phenol-chloroform extract...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.09.004
更新日期：2010-01-30 00:00:00
abstract：:The AmpFlSTR Identifiler kit has recently been accepted for use in DNA databasing of forensic samples in the FBI's National DNA Index System. In the present study, we used this kit to analyze the allele distribution of 15 short tandem repeat markers (STR) in individuals living in Caracas city, Venezuela. The allele fr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00259-7
更新日期：2003-09-09 00:00:00
abstract：OBJECTIVES:To describe the frequency of genital and anal injury and associated demographic and assault characteristics in women alleging sexual assault. DESIGN:Cross-sectional study. SETTING:Sexual Assault Resource Centre (SARC), Western Australia. PARTICIPANTS:Total of 1266 women attending SARC from Jan-2009 to Mar...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.03.013
更新日期：2017-06-01 00:00:00
abstract：:Bloodstain pattern analysts are sometimes called upon to determine the point of origin of a pattern of bloodstains. A derivation of expressions for the uncertainties deltaX and deltaY in the coordinates of the point of origin P(X, Y) of two bloodstains on a surface has recently been published. These uncertainties were...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.10.023
更新日期：2006-08-10 00:00:00
abstract：:Reddish discoloration of exposed skin areas, called frost erythema, is an important criterion for the diagnosis of fatal hypothermia. In the present study, we used immunohistochemistry in a prospective trial to show that on the molecular level, the correlate of frost erythema is hemoglobin without hemorrhage. Furtherm...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.05.029
更新日期：2006-05-10 00:00:00
abstract：:Suicide has been reported as the second or third most common cause of death in children and adolescents worldwide. In this study, cases of under the age of 19 years submitted to the Institute of Forensic Medicine, First Specialization Board between 1996 and 2000 as suicides by the Board were evaluated retrospectively....
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2003.11.024
更新日期：2004-05-28 00:00:00
abstract：:The exposition to heavy metal-rich airborne due to fire practicing has forced to the development of heavy metal-free environmental ammunition primers all over the world. Here we characterize the GSR elements present in the Brazilian lead-free ammunition produced by Companhia Brasileira de Cartuchos (CBC) and commercia...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.07.005
更新日期：2008-05-02 00:00:00
abstract：:Population genetic data of the short tandem repeat system FGA were determined by PCR analysis in two Austrian population samples, one population north of the Alps and one population south of the Alps. A total of 15 different alleles could be observed in 500 unrelated individuals. No significant differences were found ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(97)00171-0
更新日期：1998-01-09 00:00:00
