Abstract:
:Formalin-fixed and paraffin-embedded tissue (FF-PET) is an invaluable resource for retrospective molecular genetic studies, but the extraction of high-quality genomic DNA from FF-PET is still a problematic issue. Despite the range of DNA extraction methods currently in use, the association of phenol-chloroform extraction and silica-based purification protocols, reported in ancient DNA studies on archaeological bones, has, to our knowledge, not been used for DNA extraction from FF-PET yet. The present study compared the efficiency of three DNA extraction and purification protocols from two different FF-PET substrates, heart and liver, by using quantitative PCR and multiplex amplification. We showed that the method, using phenol-chloroform and the QIAamp DNA mini Kit (Qiagen), was the most effective DNA extraction and purification method and that the DNA quantity extracted from liver is statistically more important than that extracted from heart. Autosomal STR typing by multiplex amplifications gave partial allelic profiles with only small size products (less than 300 bases) amplified, suggesting that DNA extracted from FF-PET was degraded. In conclusion, the protocol presented here, previously described in studies on ancient bones, should find application in different molecular studies involving FF-PET material.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Farrugia A,Keyser C,Ludes Bdoi
10.1016/j.forsciint.2009.09.004subject
Has Abstractpub_date
2010-01-30 00:00:00pages
e25-8issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(09)00366-1journal_volume
194pub_type
杂志文章abstract::Postmortem biochemical indices may provide a useful adjunct to morphological studies in the identification of antemortem brain insult. We studied 34 routine medico-legal cases categorising them into one of four diagnostic groups. There were 11 cases of head trauma, 7 of 'hypoxia' (3 hangings and 4 carbon monoxide or d...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90107-8
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pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.05.002
更新日期:2018-07-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.02.016
更新日期:2005-07-16 00:00:00
abstract:INTRODUCTION:Age assessment based on dental development is often requested in order to assess whether an individual is older or younger than 18 years of age. There are several statistical approaches to estimate age based upon third molar development. The aim of this study was to apply the principles of transition analy...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110145
更新日期:2020-03-01 00:00:00
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journal_title:Forensic science international
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doi:10.1016/0379-0738(78)90025-7
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(92)90197-5
更新日期:1992-03-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00183-4
更新日期:2002-08-28 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(98)00132-7
更新日期:1998-10-12 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.03.048
更新日期:2019-07-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.05.036
更新日期:2019-08-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.08.044
更新日期:2012-11-30 00:00:00
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journal_title:Forensic science international
pub_type: 历史文章,杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.10.013
更新日期:2013-07-10 00:00:00
abstract::An accurate and rapid screening test for nine quaternary ammonium compounds (suxamethonium chloride, pancuronium bromide, ambenonium chloride, benzethonium chloride, distigmine bromide, methylbenactyzium bromide, neostigmine bromide, propantheline bromide and pyridostigmine bromide) by direct inlet electron impact ion...
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pub_type: 杂志文章
doi:10.1016/0379-0738(91)90213-3
更新日期:1991-10-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.07.004
更新日期:2007-05-24 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00110-5
更新日期:2003-06-24 00:00:00
abstract::A fraction of SIDS cases have death delayed by successful CPR, yet they have not been compared to SIDS cases which were found dead or not successfully resuscitated. Our aims were to: (1) determine the percent of SIDS cases in the San Diego SIDS Research Project database for whom death was delayed by CPR and subsequent...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.09.009
更新日期:2008-04-07 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2009.04.017
更新日期:2009-08-10 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110139
更新日期:2020-02-01 00:00:00
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更新日期:1993-12-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90215-0
更新日期:1988-01-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.09.023
更新日期:2014-12-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2003.12.008
更新日期:2004-04-20 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00203-7
更新日期:2002-09-10 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00383-2
更新日期:2001-06-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109910
更新日期:2019-09-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)02110-5
更新日期:1997-04-18 00:00:00
abstract::A large-scale study of facial soft tissue depths of Caucasian adults was conducted. Over a 2-years period, 967 Caucasian subjects of both sexes, varying age and varying body mass index (BMI) were studied. A user-friendly and mobile ultrasound-based system was used to measure, in about 20min per subject, the soft tissu...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.034
更新日期:2006-05-15 00:00:00
abstract::Nineteen Y-chromosomal short tandem repeats (STRs), DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS388, DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, DYS460, DYS461 and DYS462 were typed in Inuit (n=70) and Danish (n=62) population samples. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00036-7
更新日期:2003-04-08 00:00:00