Abstract:
:A new method was developed for the simultaneous detection and quantitation of 6-acetyl-morphine (MAM), amphetamine, benzoylecgonine (BZE), cocaine, codeine, dihydrocodeine, EDDP (methadone metabolite), methadone and morphine in hair. The hair samples were washed, cut into 2-cm segments, pulverized, incubated with phosphate buffer and beta-glucuronidase/aryl-sulfatase. After solid phase extraction and derivatization with pentafluoropropionic anhydride/pentafluoropropanol, the drugs were identified and measured by gas chromatography/mass spectrometry using their deuterated analogues as internal standards. The method is reproducible with detection limits under 0.1 ng/mg hair for almost all substances tested. Fifteen hair samples from five subjects of a methadone treatment program were collected in a 6-month period. The hair samples were segmented and examined for methadone, its main metabolite EDDP, and drugs of abuse. Of the 96 segments analysed, 95% were positive for methadone (mean value, 10.9 ng/mg), 76% for the metabolite EDDP (mean value, 1.2 ng/mg), 69% for opiates (mean values, MAM, 7.3 ng/mg; morphine, 2.9 ng/mg; codeine, 1.0 ng/mg) and 43% for cocaine (mean values, cocaine, 2.6 ng/mg; BZE, 1.1 ng/mg). A correlation of 0.63 was found between administered methadone dosages and concentrations measured by hair analysis. Further investigation is needed to clarify interindividual differences.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Moeller MR,Fey P,Wennig Rdoi
10.1016/0379-0738(93)90273-dsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
185-206issue
1-3eissn
0379-0738issn
1872-6283pii
0379-0738(93)90273-Djournal_volume
63pub_type
杂志文章abstract::A new method of identifying human skin from a tissue fragment by detection of squamous cell carcinoma-related (SCC) antigen, using an enzyme immunoassay, was developed. When an extract was prepared from 0.1 g human skin homogenized with 1 ml of phosphate buffered saline, this method was able to detect SCC antigen in e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(96)02022-1
更新日期:1996-12-02 00:00:00
abstract::Throughout the world, young children are worryingly found to be involved in both unintentional and intentional gun violence, rendering the forensic investigation of gun handling by children a highly important matter. The effectiveness of the PDT reaction for mapping iron traces on hands of children has been tested and...
journal_title:Forensic science international
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journal_title:Forensic science international
pub_type: 杂志文章
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journal_title:Forensic science international
pub_type: 杂志文章
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更新日期:2013-07-10 00:00:00
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pub_type: 历史文章,杂志文章,评审
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abstract::Successional patterns of insect fauna on pig carcasses were studied in southwest Virginia. The objective was to identify and qualitatively assess the major taxa of forensic importance in this region. Studies were conducted in spring and summer 2001 and 2002, and fall 2002. Over 50 taxa were collected and identified. P...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.06.041
更新日期:2005-05-28 00:00:00
abstract::Although most cases involving entomological evidence occur in urban environments and under indoor conditions, there is a lack of studies determining the insect fauna of forensic importance in those environments. In the current paper we provide the first data on the composition of the forensically important insect spec...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.12.012
更新日期:2015-03-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110673
更新日期:2021-02-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(99)00124-3
更新日期:1999-11-08 00:00:00
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journal_title:Forensic science international
pub_type: 历史文章,杂志文章
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00384-4
更新日期:2001-06-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.11.010
更新日期:2013-01-10 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.01.019
更新日期:2013-03-10 00:00:00
abstract::One hundred and ten pairs of blood and semen samples and their stains were studied to type glyoxalase 1 (GLO 1) isoenzymes using agarose-starch medium. A good agreement was observed between the phenotypes expressed in blood and semen samples of the same donor. No GLO 1 activity however could be demonstrated in the vag...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90185-5
更新日期:1988-05-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109916
更新日期:2019-09-01 00:00:00
abstract::Forensic pathologists are familiar with alcohol abusers, who are found dead and in whom the cause of death cannot be ascertained. In order to examine the possible role of ketoacidosis for the cause of death in this group of alcohol abusers, the concentrations of ketone bodies (acetone, acetoacetate, D-beta-hydroxybuty...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(95)01783-6
更新日期:1995-10-30 00:00:00
abstract::This work examines the synthesis of 3,4-methylenedioxy-N-methylamphetamine (MDMA) from common starting materials that may be utilised by clandestine laboratory operators. Piperonal was prepared from two common starting materials, piperine (from pepper) and vanillin (a common flavouring). Piperine was converted to pipe...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.10.006
更新日期:2012-11-30 00:00:00
abstract::Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven d...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(03)00267-6
更新日期:2003-10-14 00:00:00
abstract::A simple and rapid method for analysis of free and conjugated cresols in biological fluids was developed. Prior to and following freeing of the conjugated cresols by acid hydrolysis in a sealed ampoule, free cresols were extracted by Extrelut column extraction, determined by gas chromatography, and confirmed by gas ch...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(90)90282-4
更新日期:1990-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.06.017
更新日期:2011-03-20 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.10.019
更新日期:2011-05-20 00:00:00
abstract::Allele- and genotype frequencies of the two short tandem repeat (STR) systems VWA and TH01 were determined in an Austrian population sample by polymerase chain reaction (PCR) analysis. A total of 9 alleles for VWA and 6 alleles for TH01 could be observed in a population of 278 (VWA) and 276 (TH01) individuals. Both sy...
journal_title:Forensic science international
pub_type: 杂志文章
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更新日期:1997-06-23 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.11.005
更新日期:2015-01-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.08.014
更新日期:2016-10-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(90)90179-3
更新日期:1990-04-01 00:00:00
abstract::The aim of this work was to study the efficiency of automatic fibre searching with the Maxcan fibre finder (Cox Analytical Systems, Sweden) in comparison to manual searching. The influence of some parameters (color, thickness, background noise) on the results of a fibre search was considered. Eighteen experimental tap...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.08.015
更新日期:2006-07-13 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.04.079
更新日期:2005-02-10 00:00:00
abstract::A challenging step in medical, veterinary and forensic entomology casework is the rapid and accurate identification of insects to estimate the period of insect activity (PIA), which usually approximates the post-mortem interval (PMI). The morphological identification of insect evidence is hampered by species similarit...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.07.018
更新日期:2014-09-01 00:00:00