Abstract：
:Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven difficult. Here, we describe the use of laser capture microdissection (LM) for the isolation of spermatozoa from microscope slides containing sperms and vaginal cells. Such slides are frequently an important source of evidential material during the forensic investigation of rape and other sexual assaults. Low copy number (LCN) PCR was used to compare profiles of sperm DNA prepared using LM and preferential lysis. LM was found to outperform preferential lysis in 15 out of 16 samples. The application of LM to the processing of actual casework slides, and in particular the potential use of LM for the analysis of old cases, is discussed. Finally, 77 post-coital slides were processed in order to accurately assess the robustness of the technique. There was a significant association between the quality of the male profile recovered and time since intercourse that was independent of the number of sperms analysed, suggesting that the DNA was degraded even though the spermhead was intact.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Elliott K,Hill DS,Lambert C,Burroughes TR,Gill Pdoi
10.1016/s0379-0738(03)00267-6keywords:
subject
Has Abstractpub_date
2003-10-14 00:00:00pages
28-36issue
1eissn
0379-0738issn
1872-6283pii
S0379073803002676journal_volume
137pub_type
杂志文章abstract：:Neonatal hair is a clinically important toxicological matrix, as it allows determination of in utero drug exposure. This paper serves to review the physiological development of the hair follicle and hair production during fetal life. An understanding of the mechanisms and timing of hair development in the prenatal per...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.12.024
更新日期：2010-03-20 00:00:00
abstract：:In 2017, 47,600 overdose deaths were reported to be associated with the abuse of opioids, including prescription painkillers (e.g. oxycodone), opiates (e.g. heroin), or synthetic opioids (e.g. fentanyl) within the United States. The recent spike in the presence of synthetic opioids in lots of heroin distributed on the...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.04.019
更新日期：2019-07-01 00:00:00
abstract：:When tried in court, mothers accused of neonaticide may claim that the umbilical cord just broke during birth and the newborn child bled to death accordingly. To evaluate the possibility of a breakage of the umbilical cord is the goal of this work. Therefore 25 umbilical cords from neonates of both sexes born at term ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.12.002
更新日期：2014-03-01 00:00:00
abstract：:Fluvoxamine is a selective serotonin reuptake inhibitor that has been considered relatively safe in overdose. At therapeutic and supratherapeutic concentrations, fluvoxamine affects cardiac conduction, prolongs QTc interval, causes hypotension, obtundation, and can increase propensity for seizures. A man in his 60s wa...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.02.047
更新日期：2019-07-01 00:00:00
abstract：:Dental forensic identifications based on comparison of antemortem and postmortem radiographs provide effective and reliable evidence. There are no standardized procedures for assessing similarities between different types of dental radiographs (e.g. orthopantomograms, bitewings, and periapical radiographs), and the op...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.06.015
更新日期：2012-10-10 00:00:00
abstract：:Reported here is a very rare case in which the bruises were caused to the lower anterior region of the right thigh by the impact of the rim of a scooter's seat made up of a slightly flexible plastic sheet covered with artificial leather. The bruises depicted the pattern of the subjacent lower end of the femur and the ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.01.016
更新日期：2009-04-15 00:00:00
abstract：:An extensive literature review and new post-irradiation experimental results are presented of genotyping blood stains and hair, and physical examinations of latent fingerprints, hairs, and fibers. Results indicate that successful development of nuclear short tandem repeat (STR) and mitochondrial DNA sequence profiles ...
journal_title：Forensic science international
pub_type： 杂志文章,评审
doi：10.1016/j.forsciint.2018.01.012
更新日期：2018-03-01 00:00:00
abstract：:Gladiator remains from a recently unearthed cemetery in ancient Ephesus (Turkey) offer a unique opportunity for proving common theories involving the weaponry and techniques of gladiator fighting based on the evidence supplied by cranial bones. This mass grave is the first of its kind to undergo a thorough osteologica...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2005.10.010
更新日期：2006-07-13 00:00:00
abstract：:Numerous studies have mentioned to the high percentage of violent deaths in prison psychiatric hospitals, with suicide being the principal cause. The aim of this study was to analyze the circumstances related with the deaths recorded in a high security institution. Postmortem reports on all the deaths at the Alicante ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00441-2
更新日期：2001-07-15 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(83)90129-9
更新日期：1983-05-01 00:00:00
abstract：:During the past decade, several studies have been carried out using the calcaneus bone for sex estimation. This paper collected data using ten variables for metric characteristics of the calcaneus of 144 modern Cretans and examined their correlation with known sex. Secondly, the formulae developed by Peckmann et al. (...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.04.005
更新日期：2017-08-01 00:00:00
abstract：:Forensic investigators commonly interpret bone fracture patterns to estimate the force required to generate that trauma. Unfortunately, these estimates are limited to qualitative values such as "mild", "moderate" or "extreme" force. This work presents a new experimental forensic device developed to simulate blunt- and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.110123
更新日期：2020-02-01 00:00:00
abstract：:Several commercial PCR multiplex kits incorporate the amelogenin locus for the purpose of human gender identification. Consequently, erroneous results in the electropherogram profile of this locus can carry important forensic implications. In this study, dropout of the amelogenin Y allele was detected in 5 out of 77 p...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.05.002
更新日期：2007-03-02 00:00:00
abstract：:The effects of absorption time, titer of antiserum or lectin in the absorption stage, and elution temperature on relative antibody or lectin yield in elutes in the absorption-elution procedure were studied in bloodstains and ammoniacal extracts of bloodstains using conventional grouping reagents. In addition, monoclon...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(86)90182-9
更新日期：1986-07-14 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(89)90236-3
更新日期：1989-04-01 00:00:00
abstract：:Toluene is the most common volatile used for sniffing among adolescents. During 1983-1987, 114 drivers were arrested in Norway with blood toluene concentrations (BTCs) greater than 10 microM. Only four of these drivers were women. The age range was 15-34 years, and the mean age was 21. The mean BTC was 109 microM. The...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90170-4
更新日期：1990-01-01 00:00:00
abstract：:The Mombello Psychiatric Hospital in Limbiate, near Milan, replaced the old Senavra Hospital as the Psychiatric Hospital for the Province of Milan in the 19th century. During the early 20th century, bodies of several Mombello patients were dissected and preserved by Giuseppe Paravicini, an anatomist who operated withi...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2016.11.011
更新日期：2017-01-01 00:00:00
abstract：:Preliminary results concerning the use of liquid crystals (LC) in fingerprinting are given. These were based on thin layers of liquid crystals, changing colours (observed in linear polarized light) due to dissolved organic matter, transferred from fingers' surfaces. The possibility is shown of using LC to illustrate n...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(91)90137-8
更新日期：1991-07-01 00:00:00
abstract：:A comparison study of the American style ASP extendible police baton with the conventional UK police wooden truncheon was performed. It was found that the ASP baton generated approximately twice the contact pressure than the conventional wooden truncheon. A discussion on the medical/legal aspects of the results are gi...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)90411-1
更新日期：1994-06-28 00:00:00
abstract：:A sandwich ELISA for human prostate-specific antigen (PSA) is described. Optimal assay conditions, resulting in a sensitive assay with a low background, are presented. The method uses a hyperimmune antiserum produced in the New Zealand white rabbit, against human semen PSA. The IgG fraction of the antiserum was conjug...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(91)90141-5
更新日期：1991-07-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.12.007
更新日期：2012-06-10 00:00:00
abstract：:This study focused on the frequency of pathological acid gastroesophageal reflux (AGER) on 50 children considered to be at risk for sudden infant death syndrome: that is to say, 30 near misses and 20 subsequent siblings. Four parameters were studied in a 24-h pH metry: (1) The percentage of time spent at pH less than ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(88)90152-1
更新日期：1988-02-01 00:00:00
abstract：:The castor bean plant (Ricinus communis L.) or wonder tree is cultivated in many countries as an ornamental annual plant in gardens. The highest concentration of the lectin ricin is present in the seeds and pods. Ricin is considered as one of the most toxic natural poisons. Ricinine is a piperidine alkaloidal toxin pr...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.04.019
更新日期：2009-08-10 00:00:00
abstract：:We have performed a population genetic study on a population from South Saxony-Anhalt, Germany. The allele distributions of the systems DYS19, DYS385, DYS389I/II DYS390, DYS391, DYS392 and DYS393 were investigated in a sample of 234 unrelated males. PCR products were detected using capillary electrophoresis on the ABI...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.01.004
更新日期：2005-12-20 00:00:00
abstract：:Most traditional techniques to recover latent fingermarks from metallic surfaces do not consider the metal surface properties and instead focus on the fingermark chemistry. The scanning Kelvin probe (SKP) technique is a non-contact, non-destructive method, used under ambient conditions, which can be utilised to recove...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.07.025
更新日期：2018-10-01 00:00:00
abstract：:Venlafaxine is a phenethylamine antidepressant which inhibits both serotonin and norepinephrine reuptake and is structurally unrelated to the serotonin reuptake inhibitors (SSRIs). Its major metabolite, O-desmethylvenlafaxine (ODV), also inhibits serotonin reuptake. Although metabolized by the cytochrome P-450 (CYP) s...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(01)00455-8
更新日期：2001-09-15 00:00:00
abstract：:Successional patterns of insect fauna on pig carcasses were studied in southwest Virginia. The objective was to identify and qualitatively assess the major taxa of forensic importance in this region. Studies were conducted in spring and summer 2001 and 2002, and fall 2002. Over 50 taxa were collected and identified. P...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.06.041
更新日期：2005-05-28 00:00:00
abstract：:The case of a 13-month-old boy with a diagnosis of unclear unconsciousness is reported on. As the physical examination did not lead to any explanation of his condition, the administration of drugs in the context of Munchausen syndrome by proxy was suspected. Complex forensic-toxicological analyses using HPLC/UV, LC/MS...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.04.021
更新日期：2009-08-10 00:00:00
abstract：:As part of a long-term project on Northeastern Brazilians, population genetic data were obtained from 323 unrelated individuals from the state of Paraíba. The loci studied were CSF1P0, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D18S51, D21S11, D8S1179, F13A01, F13B and LPL. Their distributions are in Hardy-Weinberg eq...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2006.10.026
更新日期：2007-12-20 00:00:00
abstract：:Since gamma-hydroxybutyrate (GHB) is present in both blood and urine of the general population as an endogenous compound, toxicologists must be able to discriminate between these endogenous levels and a concentration resulting from exogenous exposure. The implementation of a cut-off concentration must be done cautious...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.02.036
更新日期：2004-07-16 00:00:00
