Abstract:
:A sandwich ELISA for human prostate-specific antigen (PSA) is described. Optimal assay conditions, resulting in a sensitive assay with a low background, are presented. The method uses a hyperimmune antiserum produced in the New Zealand white rabbit, against human semen PSA. The IgG fraction of the antiserum was conjugated with horseradish peroxidase and used in the sandwich ELISA method. The anti-PSA IgG showed no cross reactions with saliva, normal blood, female urine, vaginal fluid, or menstrual blood. On occasions, a blood sample showed a non-specific cross-reaction, which was detected by non-immune rabbit IgG. This reaction could be caused by rheumatoid factors, as indicated by experiments with a series of known IgG and IgM rheumatoid antibodies, although other heterophilic antibodies could not be eliminated. The recovery of PSA added to blood plasma, saliva and vaginal fluid was affected by three factors; (a) protein concentration (dilution) of body fluid; (b) nature of the protein; and (c) amount of PSA added.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Stowell LI,Sharman LE,Hamel Kdoi
10.1016/0379-0738(91)90141-5subject
Has Abstractpub_date
1991-07-01 00:00:00pages
125-38issue
1eissn
0379-0738issn
1872-6283pii
0379-0738(91)90141-5journal_volume
50pub_type
杂志文章abstract::Whole exome sequencing (WES) and bioinformatics analysis were used to investigate potential disease-causing gene mutations in a sudden unexplained death syndrome (SUDS) case after autopsy and pathology tests failed to suggest an obvious disease mechanism. Following whole exome sequencing, a 3-step bioinformatics filte...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.08.022
更新日期:2015-12-01 00:00:00
abstract::This paper presents a novel digital watermarking technique using face and demographic text data as multiple watermarks for verifying the chain of custody and protecting the integrity of a fingerprint image. The watermarks are embedded in selected texture regions of a fingerprint image using discrete wavelet transform....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.08.019
更新日期:2007-07-04 00:00:00
abstract::This article studies teenage homicide victims in Metro-Dade County, Miami, FL, U.S.A., between the years 1973 through 1982. A total of 263 cases were selected. Cases were then analyzed with respect to age, race, sex, cause of death, scene circumstances, alcohol and drugs detected at autopsy. A discussion follows that ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(84)90190-7
更新日期:1984-07-01 00:00:00
abstract::Physical fit examinations have long played a critical role in forensic science, particularly in the trace evidence, toolmark, and questioned documents disciplines. Specifically, in trace evidence, physical fits arise in various instances such as separated pieces of duct tape, torn textile fragments, and fractured poly...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110349
更新日期:2020-08-01 00:00:00
abstract::Extrahepatic biliary atresia (EHBA) is a rare disease characterized by progressive and obliterative cholangiopathy in infants and is one of the major causes of secondary vitamin K deficiency bleeding (VKDB) due to cholestasis-induced fat malabsorption. Breast feeding increases the tendency of bleeding in EHBA patients...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.04.018
更新日期:2012-09-10 00:00:00
abstract::In this report we describe a first suicide case in Serbia related to ingestion of Taxus baccata leaves. A 30-year old woman was found dead, and the green plant material in a plastic bag was found near her bed. Autopsy revealed dark green needle-like leaves in the stomach, similar to that contained in the plastic bag, ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.07.017
更新日期:2018-09-01 00:00:00
abstract::Suicide has been reported as the second or third most common cause of death in children and adolescents worldwide. In this study, cases of under the age of 19 years submitted to the Institute of Forensic Medicine, First Specialization Board between 1996 and 2000 as suicides by the Board were evaluated retrospectively....
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2003.11.024
更新日期:2004-05-28 00:00:00
abstract::Laboratory investigations were conducted to evaluate the effect of ultraviolet radiation components and solar radiation exposure as a function of time on the degradation of whole human blood DNA from the standpoint of forensic analysis. Ten μL of whole human male blood samples were exposed to UV-A, UV-B, UV-C, and sol...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110674
更新日期:2021-02-01 00:00:00
abstract::A decline in 3,4-methylenedioxy-N-methylamphetamine (MDMA) use in Adelaide, Australia from 2009 to 2010 was confirmed by us previously. Reports suggested that the shortage in MDMA supply was associated with an increased prevalence of other synthetic stimulants, but quantitative measurements were unavailable. To obtain...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.06.007
更新日期:2013-09-10 00:00:00
abstract::In prosecution, Belgian judiciary currently uses outdated yield figures (28.1g per plant, sold at € 3/g at grower level) for fining illicit indoor cannabis plantations. Using state-of-the-art cultivation techniques, our growth experiments showed that yield is better expressed in g/m(2) cultivated surface area rather t...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.03.013
更新日期:2012-07-10 00:00:00
abstract::In a study of the incidence of suicide in jails and prisons under increasing conditions of deprivation in Italy from 2004 to 2008, imprisonment involving greater levels of isolation was found to be associated with a higher rate of suicide. Greater care should be taken to ensure that prisoners in isolation do not commi...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.08.016
更新日期:2013-12-10 00:00:00
abstract::Due to inherent differences between terrestrial and aquatic systems, methods for estimating the postmortem interval (PMI) are not directly applicable to remains recovered from water. Recent studies have explored the use of microbial succession for estimating the postmortem submersion interval (PMSI); however, a non-di...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110480
更新日期:2021-01-01 00:00:00
abstract::In this paper we propose an innovative methodology for automated profiling of illicit tablets by their surface granularity; a feature previously unexamined for this purpose. We make use of the tiny inconsistencies at the tablet surface, referred to as speckles, to generate a quantitative granularity profile of tablets...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.03.008
更新日期:2011-07-15 00:00:00
abstract::Traces of the improvised explosive urea nitrate can be characterized by a sensitive colorimetric reaction with p-dimethylaminocinnamalaldehyde (p-DMAC, UN-1 reagent). As recently shown, the dark red product has a structure of a protonated Schiff base. The unprotonated free base, previously postulated in the literature...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.10.017
更新日期:2011-05-20 00:00:00
abstract::This paper describes a repeatability test, comparing conventional X-ray diffraction (XRD) analyses with the technique of quantitative X-ray diffraction (QXRD) as a determinant of mineral abundance. The conjunctive use of both methods allows specific mineral abundances to be matched between scene of crime (SOC) and sus...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.03.017
更新日期:2004-10-04 00:00:00
abstract:BACKGROUND:Studies testing the Willems method of age estimation with gender-specific scores on different population groups have reported consistently more accurate results over other methods. Not enough is known about the validity of Willems non-gender specific scores. AIM:The present study aimed to compare the accura...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.05.012
更新日期:2016-09-01 00:00:00
abstract::In this work, a case of deliberate self-poisoning is presented. A 14-year-old girl suddenly died during one of the several hospitalizations. Abdominal computer tomography showed a large number of metallic particles in the large intestine. Analysis of blood and internal organs for mercury and other toxic metals carried...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.02.015
更新日期:2014-04-01 00:00:00
abstract::The forensic anthropologist is responsible for contributing to the identification of an unknown by constructing a biological profile from their skeletal remains. Towards achieving this goal, anthropologists can apply population and temporally specific standards with known error margins to morphometric data collected f...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.08.019
更新日期:2014-11-01 00:00:00
abstract::Organic impurities of methamphetamine may show different patterns by synthesis. In the present study, we tried to find the impurities reflecting the conditions of synthesis by comparing impurity patterns of the methamphetamine samples synthesized by different methods. Sixteen methamphetamine samples were synthesized f...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.054
更新日期:2006-09-12 00:00:00
abstract::In cases of disputed parentage the biostatistical information is contained in the frequencies X and Y (as defined by Essen-M oller ); X denotes the hypothesis "paternity", Y the hypothesis "non-paternity". Essen-M oller proposed a probability of paternity which includes both values: W = X/(X + Y) (where X + Y becomes ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(84)90010-0
更新日期:1984-05-01 00:00:00
abstract::This study investigates the enhancement of footwear impressions prepared with soils from different locations on a variety of fabric surfaces with different morphology. Preliminary experiments using seventeen techniques were carried out and the best responding reagents were evaluated further. Results indicated that the...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.11.011
更新日期:2012-06-10 00:00:00
abstract::Though the child abuse syndrome is usually considered to have been first described in the middle of the present century, references to child abuse occur throughout history and in the 19th century, detailed descriptions were published in the French medical literature. ...
journal_title:Forensic science international
pub_type: 历史文章,杂志文章
doi:10.1016/0379-0738(86)90008-3
更新日期:1986-02-01 00:00:00
abstract::Despite the fact that the differences in epidermal ridge density between men and women have been accepted for some time, they have only been thoroughly demonstrated in a small number of populations. The aim of this study is to determine whether such differences exist in a sample of the Spanish population by counting e...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2008.06.014
更新日期:2008-08-25 00:00:00
abstract::The aim of the study was to improve the objectivity of X-ray image comparison for the identification of unknown dead individuals. CT-data were collected for 30 macerated skulls. An already presented computer program which uses CT data to establish virtual X-ray images was used to obtain X-rays with different beam angu...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00452-7
更新日期:2001-03-01 00:00:00
abstract::Many document examiners recognize the importance of an individuals 'state of mind' and its effect on handwriting but historically have steered clear of working closely with psychiatrists, linguists, or psychologists in understanding the interaction of nervous impulses and motor responses on the final handwritten produ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(82)90150-5
更新日期:1982-01-01 00:00:00
abstract::In this article, the authors present a case of intrauterine foetal death (IUFD). The post-mortem histologic examination revealed placental mesenchymal dysplasia (PMD), a rare human placental disorder. Moreover, cases of PMD are often misdiagnosed as partial mole. The mother was a 26-year-old Italian, whose pregnancy, ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.04.002
更新日期:2013-06-10 00:00:00
abstract:OBJECTIVE:To evaluate the benefits of using serial measurements of plasma cholinesterase (butyrylcholinesterase, BuChE) activity in the management of cholinesterase inhibitor insecticidal poisoning. METHOD:After establishing and validating BuChE activity test, and making it available for clinical service in the toxico...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.06.074
更新日期:2006-10-16 00:00:00
abstract::In forensics and archaeology, it is important to distinguish human from animal remains and to identify animal species from fragmentary bones and bloodstains. We report blind tests in which a protein radioimmunoassay (pRIA) was used to identify the species of six bone fragments lacking morphological specificity and 43 ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.08.007
更新日期:2006-06-02 00:00:00
abstract::A postmortem root band (PMRB) is a distinct microscopic feature that is postulated to occur in hair remaining in the follicle during the postmortem interval [1] (Petraco et al., 1998). The scientific validity of this premise has been highlighted in two recent high-profile criminal cases involving PMRBs [2,3] (State of...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.07.009
更新日期:2016-10-01 00:00:00
abstract::Analysis of cannabis has gained new importance worldwide, mainly for quality control within the legalized recreational and medical cannabis industry, but also for forensic differentiation between drug-type cannabis and legal products such as fiber hemp and CBD-rich/THC-poor cannabis. We herein present an HPLC-DAD meth...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.03.046
更新日期:2019-06-01 00:00:00