Abstract：
:The OKT3 antibody activates locomotion of human blood T lymphocytes as measured by polarization assays and invasion of collagen gels. The proportion of motile cells increases during a period of 24-48 hr of culture, even following only a brief initial contact with OKT3. The motile cells are the growing population. Locomotion activation is cell-density dependent. Studies with surface-bound mitogens, namely substratum-bound OKT3 and Con A-Sepharose, showed that only lymphocytes in direct contact with the mitogen acquired locomotor capacity. Those separated from it by a cell-impermeable filter were not activated. The response to OKT3 requires the whole antibody molecule. F(ab')2 fragments were inactive. Intact normal human IgG, but not its F(ab')2 fragments, blocked the response. Removal of RFc gamma + cells from the population by rosetting with IgG-Ab-coated sheep red cells prevented the response. These findings suggest that an RFc gamma + population has to be present for T cells to become activated to locomotion by OKT3, and that the OKT3 antibody links the T cell to an FcR+ cell, cell-to-cell contact being essential for activating the locomotor response.
journal_name
Immunologyjournal_title
Immunologyauthors
Wilkinson PC,Higgins Asubject
Has Abstractpub_date
1987-03-01 00:00:00pages
445-51issue
3eissn
0019-2805issn
1365-2567journal_volume
60pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Male lymphocytic choriomeningitis (LCM) virus-carrier mice were mated to uninfected females and their offsprings tested for (i) LCMV virus and (ii) their potential to generate LCM virus-specific cytotoxic T cells. Among about 500 offspring tested no example of antigen-independent inheritance of tolerance to LCM virus ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Different cell types, including dendritic cells, macrophages and Ia+ B cells, have been described to present soluble antigen (Ag) to T-cell hybridomas. However, it is still not clear whether these different cell types can act as antigen-presenting cells (APC) for complex and insoluble Ag such as viral particles. Using...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:We previously identified a cis-acting element within the 3' untranslated region of CD40 ligand messenger RNA (mRNA) that is composed of three complex binding sites and acts to increase mRNA stability in both in vitro and in vivo systems. We now demonstrate the functional consequences of the three binding sites with re...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02783.x
更新日期：2008-07-01 00:00:00
abstract：:Haptoglobin (Hp), a major acute-phase plasma protein, has been found in arthritic synovial fluid (SF). However, the function and structural modifications of Hp in arthritic SF are unknown. To investigate in vivo generation of modified Hp associated with inflammatory disease, we examined a new Hp isoform in SF from pat...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12125
更新日期：2013-09-01 00:00:00
abstract：:Human autologous-rosette-forming T cells (Tar cells) have many of the characteristics of post-thymic precursor cells. Thus, they bind to sheep erythrocytes but have neither receptors for the Fc portion of IgG nor for that of IgM. They include a subpopulation that binds peanut agglutinin which suggests that they are im...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:A panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. A striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the CD45...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Major histocompatibility complex (MHC) alleles acting as immune response genes are coexpressed in heterozygous individuals and therefore control of immune responses is usually codominant. As an exception to this rule, however, several examples of recessive immune responses have been ascribed to regulatory, e.g. suppre...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00956.x
更新日期：2000-02-01 00:00:00
abstract：:Rabbit IgG and its Fab, Fc and pFc' fragments, prepared by papain or peptic digestion, were assayed for binding to homologous peritoneal macrophages. The binding affinity of IgG for the peritoneal macrophages (Ka = 5.9 +/- 1.6 x 10(5) L/M) was comparable to that recorded with alveolar macrophages (7.6 +/- 1.8 x 10(5) ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:A soluble form of Fc epsilon RII/CD23 is spontaneously released from most lymphoblastoid cell lines established by the Epstein-Barr virus (EBV). Such a product was purified on an IgE-Sepharose column and its pyrogenic effect was investigated in rabbits. This preparation induced a monophasic fever in rabbits, with a pe...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Phagocytes such as dendritic cells (DC) and macrophages employ phagocytosis to take up pathogenic bacteria into phagosomes, digest the bacteria and present the bacteria-derived peptide antigens to the adaptive immunity. Hence, efficient antigen presentation depends greatly on a well-regulated phagocytosis process. Lip...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12051
更新日期：2013-05-01 00:00:00
abstract：:The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:Docosahexaenoic (22:6 n-3) and eicosapentaenoic acid (20:5 n-3) stimulated the oxygen-dependent respiratory burst in intact neutrophils in a dose-dependent manner as measured by either superoxide dismutase (SOD)-inhibitable cytochrome c reduction and lucigenin-dependent chemiluminescence. A number of longer chain hexa...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Colorectal cancer is considered a non-immunogenic malignany. One strategy to augment the immunogenicity of such tumours is represented by the expression of costimulatory molecules by gene transfer. Using transfected variants of the human colorectal cancer cell line SW480 we tested various costimulatory molecules (CD80...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1998.00450.x
更新日期：1998-03-01 00:00:00
abstract：:The immunosuppressive effect of cyclosporin A (CS-A) was investigated in RIC-Sprague-Dawley rats. In vivo, CS-A totally abolished the formation of antibodies to the hapten dinitrophenyl (DNP) in rats immunized with DNP-keyhole limpet haemocyanin. In vitro, the effect of CS-A was investigated in spleen cell cultures st...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:The requirement for activity of the enzyme ADP-ribosyl transferase (ADPRT) and changes in single-strand DNA breaks were assessed during the initial stimulation of quiescent murine splenic lymphocytes with mitogen alone, the stimulation of activated blasts with IL-2-containing medium and, for comparison, the serum stim...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Microglia cells, the resident innate immune cells in the brain, are highly active, extending and retracting highly motile processes through which they continuously survey their microenvironment for 'danger signals' and interact dynamically with surrounding cells. Upon sensing changes in their central nervous system mi...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12177
更新日期：2014-03-01 00:00:00
abstract：:In many species, the rejection of liver allografts is milder than that of other organs. This is especially so in the rat where, without immunosuppressive treatment, liver grafts between certain strain combinations are accepted permanently, whereas skin, heart and renal allografts undergo acute rejection. Reliable surg...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：
更新日期：1985-07-01 00:00:00
abstract：:The effect of invasive bacteria on the release of proinflammatory mediators (oxygen radicals, leukotriene release) from human polymorphonuclear neutrophils was studied. Bacterial stimuli were used including genetically cloned invasive Yersinia enterocolitica strains 108-P (bearing the phagocytosis-resistance plasmid) ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:Bacillus Calmette-Guérin (BCG) inoculation has been shown to inhibit certain immune functions. To determine whether this inhibition adversely affects host defences against infection, the effect of BCG on Listeria infection in mice was investigated. Mice were injected intravenously (i.v.) with Listeria monocytogenes an...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:The effects of anti-Ia antibodies and cyclosporin A (CsA) on phenotypic differentiation of murine thymocytes were assessed in organ-cultured fetal thymuses. Both agents specifically abrogated the generation of CD4+8- thymocytes. Immunohistochemical studies revealed that Ia antigen on the organ-cultured thymic epitheli...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Studies in long-term non-progressors (LTNP) have suggested that the quality of the CD8(+) response may involve protective human leucocyte antigen (HLA) class I alleles. However, studies examining the expansion ability of different functional CD8(+) T cells and their association with HLA class I alleles are lacking. LT...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03490.x
更新日期：2011-11-01 00:00:00
abstract：:An autoreactive T-cell clone, MTg9B3, specific for mouse thyroglobulin, has been characterized. This clone has properties consistent with it an being autoreactive T helper cell. It is I-A restricted, with a surface phenotype of Thy 1+, L3T4+, Ly2-, and produces a number of non-specific lymphokine activities following ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:We have previously shown that a subset of human osteoblast-like bone surface cells (HBC) derived from explants of trabecular bone constitutively express class II major histocompatibility complex (MHC) determinants in long-term culture. We now examine the capacity of HBC to stimulate allogeneic and autologous periphera...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:Human blood monocytes were activated by bacterial lipopolysaccharide endotoxin (LPS) (10 ng/ml) for cytotoxicity of WEHI-164 mouse fibrosarcoma cells, determined by release of 51Cr from WEHI-164 tumour cells incubated with monocyte supernatants. The chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (FMLP) aug...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Using KOLT-2 monoclonal antibody (mAb) recognizing a 44,000 molecular weight (MW) T-cell activation antigen (CD28), we observed the co-expression of KOLT-2 (CD28) antigen and Tac (CD25) antigen, associated with a 55,000 MW chain of the interleukin-2 receptor (IL-2R) complex. IL-2R (p55), on several T-cell lines transf...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:To study the influence of macrophages on the migration and distribution of lymphocytes in the spleen, macrophages were eliminated from the spleen of mice by injection of liposomes in which DMDP was encapsulated. This leads to an elimination of macrophages in both the red pulp and marginal zone of the spleen within 1-2...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP3K family. Recently, we found that MEKK3 plays a critical role in interleukin-1 (IL-1) receptor and Toll-like receptor 4 signalling using established primary mouse embryonic fibroblast (MEF)...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2006.02495.x
更新日期：2007-02-01 00:00:00
abstract：:As the burden of infectious diseases becomes reduced in many countries, a remarkable increase in the incidence of allergies has occurred. The basis for the rise in atopic disorders as a correlate of the decline in infectious diseases has not been defined. In the present study, we tested experimentally whether prior sy...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00856.x
更新日期：1999-11-01 00:00:00
abstract：:MCA44 is a monoclonal antibody (mAb) to guinea-pig decay-accelerating factor (DAF) and, using this mAb, tissue distribution of guinea-pig DAF was studied by immunofluorescence. Guinea-pig DAF was found to be expressed not only on the vascular endothelium but also on different types of cells, such as the tubular epithe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1998.00592.x
更新日期：1998-10-01 00:00:00
abstract：:We have confirmed previous observations that intestinal anaphylaxis induced in rats previously sensitized to ovalbumin (OVA) is associated with an increased uptake of an unrelated 'bystander' protein, bovine serum albumin (BSA) fed 1 hr previously. In this study, this enhanced protein uptake was associated with an inc...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
