Abstract：
:Mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP3K family. Recently, we found that MEKK3 plays a critical role in interleukin-1 (IL-1) receptor and Toll-like receptor 4 signalling using established primary mouse embryonic fibroblast (MEF) cell lines. However, the function of MEKK3 in immune cells has not been studied because germ-line MEKK3 knockout mice are embryonically lethal between embryonic days 10 and 11. In this study, we used small interference RNA to the mouse Mekk3 gene to specifically knock down MEKK3 expression in the macrophage line Raw264.7. We found that the lipopolysaccharide-induced IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) production was dramatically decreased in MEKK3 knockdown cells whereas the tumour necrosis factor-alpha and IL-1beta production were not affected. We also observed that the ERK1/2, p38 and JNK MAPK induction in MEKK3 knockdown cells were moderately inhibited within the first 60 min of stimulation, while the ERK and p38 were more severely inhibited after 2-4 hr of stimulation. Degradation of IkappaBalpha was also partially blocked in MEKK3 knockdown cells. Notably, the impairment in IL-6 and GM-CSF production in the MEKK3 knockdown cells was restored by reintroducing a human Mekk3 cDNA that could not be targeted by mouse Mekk3-siRNAs. In conclusion, this study showed that MEKK3 is a crucial and specific regulator of the proinflammatory cytokines IL-6 and GM-CSF in macrophages and provided a novel method for investigating MEKK3 function in other immune cells.
journal_name
Immunologyjournal_title
Immunologyauthors
Kim K,Duramad O,Qin XF,Su Bdoi
10.1111/j.1365-2567.2006.02495.xsubject
Has Abstractpub_date
2007-02-01 00:00:00pages
242-50issue
2eissn
0019-2805issn
1365-2567pii
IMM2495journal_volume
120pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:To assess the role of CD5 and CD28 in the pathogenesis of the decreased cellular immune function in patients with rheumatoid arthritis (RA) we analysed the expression and function of these T-cell surface molecules. The expression of CD5 as well as of CD28 in synovial and peripheral blood T cells was similar to that of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Langerhans' cells (LC) cultured for 1-3 days lose their ability to process native protein antigens but acquire the ability to stimulate resting T cells as assessed in an allogeneic mixed lymphocyte response (MLR). Lymph-borne dendritic cells (L-DC) are physiologically involved in the transport of antigens to lymph nod...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:Lipoprotein was isolated from tumour cells by sonication and ultracentrifugal flotation on KBr gradients. It contained H-2 antigen detectable by antibody binding and induced a primary or secondary cell-mediated cytotoxic response in vitro which was H-2 specific. In a syngeneic model only a secondary cell-mediated resp...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:To evaluate the role of the high-affinity monocyte receptor for lipopolysaccharide (LPS), CD14, in the process of tolerance to LPS, the human monocytic cell line Mono-Mac-6 was cultured in the absence or presence of different amounts of LPS. The kinetics of CD14 modulation in these cells showed an initial 4-day period...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Jacalin, a lectin(s) extracted from the seeds of Artocarpus integrifolia (Jackfruit), was shown to induce the production of gamma interferon (IFN gamma) in human peripheral blood mononuclear cells (PBMC) and human T-lymphocyte cultures. The amount of IFN gamma produced was enhanced in the presence of mezerein, a phorb...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:The capacity of the immune system to respond efficiently to new antigens depends upon a continuous source of naive CD4+ T cells. Such cells exit from the thymus and join the recirculated T-cell pool. Factors present at the sites of naive CD4+ T-cell circulation must be responsible for their survival, since upon remova...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00906.x
更新日期：1999-11-01 00:00:00
abstract：:Using immunohistological techniques, class 1 products of the major histocompatibility complex (MHC) have been demonstrated on various forms of human extra-villous trophoblast in placental tissues taken from first, second and third trimester pregnancies. This contrasts with the absence of class 1 MHC antigens on all fo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Vaccination strategies that can block or limit heterosexual human immunodeficiency virus (HIV) transmissions to local and systemic tissues are the goal of much research effort. Herein, in a mouse model, we aimed to determine whether the enhancement of antibody responses through mucosal and systemic immunizations, prev...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02702.x
更新日期：2008-03-01 00:00:00
abstract：:An autoreactive T-cell clone, MTg9B3, specific for mouse thyroglobulin, has been characterized. This clone has properties consistent with it an being autoreactive T helper cell. It is I-A restricted, with a surface phenotype of Thy 1+, L3T4+, Ly2-, and produces a number of non-specific lymphokine activities following ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:Eight human IgA1 myeloma proteins were analysed by SDS-PAGE. These experiments showed that purified IgA1 proteins comprise both fully S-S bonded and partly S-S bonded molecules. Pepsin digestion of the IgA1 proteins yielded three four-chain and two two-chain fragments. The four-chain fragments are likely to be derived...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:CD4 T cells help immune responses, but knowledge of how memory CD4 T cells are regulated and how they regulate adaptive immune responses and induce immunopathology is limited. Using adoptive transfer of virus-specific CD4 T cells, we show that naive CD4 T cells undergo substantial expansion following infection, but ca...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12771
更新日期：2017-10-01 00:00:00
abstract：:It is believed that self-reactive forbidden T-cell clones are generated by 'failure' of the pathway of T-cell differentiation in the thymus, if it is disturbed. We examined how such forbidden clones are generated under immunosuppressive conditions. Mice were treated with an injection of deoxyspergualin, FK506, or cycl...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00222.x
更新日期：1997-05-01 00:00:00
abstract：:The immunomodulatory molecule cyclosporine was found to cause an early acceleration of the capping of mouse B-cell membrane immunoglobulin. This was most marked when the capping process was slightly retarded by keeping the cells at 32 degrees. This is a sign that the drug can cause B-cell membrane alterations. At lowe...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:The experiments described in this paper have examined the migration of three fluorochrome-labelled T-lymphocyte subsets (CD4+, CD8+ and gamma delta+T19+) on a single passage from blood to lymph, through prescapular lymph nodes. Lymphocytes obtained from prescapular efferent lymph were labelled in vitro with fluorochro...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:In order to explore the role of glutathione in cell-mediated cytotoxicity, we have examined the effect of the sulphydryl-reactive and glutathione-depleting agent 2-cyclohexene-1-one on antibody-dependent cellular cytotoxicity, spontaneous cell-mediated cytotoxicity, and cell-mediated lympholysis by human peripheral bl...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:The migration of leucocytes into tissues is a process mediated by leucocyte endothelial interactions, in which adhesion receptors play a crucial role. Recently, it was found that 80-90% of T cells in inflammatory skin diseases were reactive to the monoclonal antibody (mAb) HECA-452+ in contrast to inflamed non-cutaneo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Variation in the sequence of T-cell epitopes between dengue virus (DENV) serotypes is believed to alter memory T-cell responses during second heterologous infections. We identified a highly conserved, novel, HLA-B57-restricted epitope on the DENV NS1 protein. We predicted higher frequencies of B57-NS1(26-34) -specific...
journal_title：Immunology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1111/imm.12161
更新日期：2014-01-01 00:00:00
abstract：:In this study we followed the in vivo fate and distribution of hapten-coupled sheep red blood cells (TNP-SRBC) coated with 125I-labelled anti-hapten antibody in mice. The majority of these complexes was rapidly taken up by the liver, and by macrophages in the marginal zone of the spleen. Within a few hours, however, m...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:Bursal and splenic lymphocytes in developing chick embryos were examined for the number of antigen binding cells (ABC) which could form rosettes with red blood cells from sheep, guinea-pig, rabbit or dinitrophenylated sheep red blood cells (DNP-SRBC). The kinetics of the development of bursal ABC to rabbit red blood c...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Highly enriched bovine polymorphonuclear neutrophils (PMN) were able to destroy herpesvirus-infected cells in the presence of complement. Our results suggest that some viral antigens are capable of activating the alternate complement pathway directly which results in cytotoxicity however, the addition of PMN plus comp...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:The fluorescence-activated cell sorter and a rosette-depletion technique were used to separate rat spleen lymphocytes and BCG-activated lymphocytes into subpopulations with and without the antigens defined by W3/13, W3/23 and OX8 monoclonal antibodies. The resultant populations were then tested for natural killer (NK)...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:In order to examine the mechanism of specific immunity in the lung, the distribution of Ia antigens and T lymphocyte populations was determined using immunoperoxidase-staining of cryostat sections of lungs from specific pathogen-free rats. BALT was found to be divided into three regions of lymphoid tissue. The central...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Chloroquine treatment of antigen-presenting cells (APC) was explored as a tool to investigate the processing pathway for major histocompatibility complex (MHC) class II-restricted presentation of the endogenous secreted hen egg lysozyme (HEL) and transmembrane measles virus haemagglutinin (HA). A 72-hr pretreatment of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:This study has examined the stimuli required for secretion of regulated upon activation, normal T-cell expressed, presumed secreted (RANTES) from T lymphocytes and found that stimuli such as phorbol 12-myristate 13-acetate (PMA), which are unable to support T-cell proliferation and interleukin-2 (IL-2) production, are...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00072.x
更新日期：2000-09-01 00:00:00
abstract：:Paratyphoid vaccine injected between 4 days and 3 hours before injection of labelled immune complexes (125-I-labelled BGG-anti-BGG), inhibits follicular trapping of these complexes in the mouse spleen. Inhibition is maximal when paratyphoid vaccine is given 1 day before, almost no label being found in the spleen folli...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:This study investigates the effect of feeding fermented milks with Lactobacillus casei, Lactobacillus acidophilus and a mixture of both micro-organisms on the specific and non-specific host defence mechanisms in Swiss mice. Animals fed with fermented milk for 8 days (100 micrograms/day) showed an increase in both phag...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Circulating CD4+ CD25+ regulatory T cells (Tregs) have been demonstrated to maintain immunotolerance and suppress the antigen-specific or antigen-non-specific T-cell responses, but their role in chronic hepatitis B (CHB) infection in humans has not been well characterized. In this study, we analysed the frequency and ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02691.x
更新日期：2008-01-01 00:00:00
abstract：:Activation of self-reactive T cells in healthy adults is prevented by the presence of autoantigen-specific CD4+CD25+ regulatory T cells (CD25+ Treg). To explore the functional development of autoantigen-reactive CD25+ Treg in humans we investigated if thymic CD25+ Treg from children aged 2 months to 11 years and cord ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02186.x
更新日期：2005-08-01 00:00:00
abstract：:An adoptive transfer assay system was used to investigate the ability of serum from tolerant, liver-grafted rats to inactivate normal thoracic duct lymphocytes. PVG rats were rendered tolerant of DA alloantigens by liver grafting. Serum taken from such rats was used to treat normal PVG thoracic duct lymphocytes (TDL) ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:The effects of anti-Ia antibodies and cyclosporin A (CsA) on phenotypic differentiation of murine thymocytes were assessed in organ-cultured fetal thymuses. Both agents specifically abrogated the generation of CD4+8- thymocytes. Immunohistochemical studies revealed that Ia antigen on the organ-cultured thymic epitheli...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
