Abstract：
:Human blood monocytes were activated by bacterial lipopolysaccharide endotoxin (LPS) (10 ng/ml) for cytotoxicity of WEHI-164 mouse fibrosarcoma cells, determined by release of 51Cr from WEHI-164 tumour cells incubated with monocyte supernatants. The chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (FMLP) augmented LPS-induced cytotoxicity but had no effect alone. FMLP but not LPS stimulated phospholipase C (PLC), determined by the release of [3H]inositol phosphates. Addition of tumour promoter and protein kinase C stimulant, phorbol-12-myristate-13-acetate (PMA) at concentrations of 3 x 10(-10) M to 3 x 10(-9) M, resulted in an augmentation of 30-200% in LPS-evoked cytotoxicity. The effects of FMLP and PMA, like the effect of LPS alone, were completely blocked by antibody to recombinant human tumour necrosis factor-alpha (TNF-alpha), indicating that cytotoxicity induced by LPS, FMLP, and PMA were due solely to TNF release. Concentrations of PMA greater than 3 x 10(-9) M caused inhibition of TNF release. Okadaic acid (20 ng/ml), an inhibitor of phosphatases I and IIa, augmented the effects of LPS and the stimulatory effects of low levels of PMA, suggesting that phosphorylation was important in the actions of both LPS and PMA. The effects of LPS and of low levels of PMA were augmented by the protein kinase C (PKC) inhibitors H-7 (10-30 microM), staurosporine (2-10 nM) and calphostin C (0.1 microM). Higher concentrations of the inhibitors prevented LPS-evoked TNF release and its augmentation by low levels of PMA. However, they did not prevent the inhibition by high levels of PMA. One possible explanation for the results is that different isozymes of PKC may mediate the stimulatory as compared to the inhibitory effects of PKC on TNF production.
journal_name
Immunologyjournal_title
Immunologyauthors
Coffey RG,Weakland LL,Alberts VAkeywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
48-54issue
1eissn
0019-2805issn
1365-2567journal_volume
76pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:The influence of the thymus and the possible requirement for surface immunoglobulin expression for the development of K and NK activity were assessed in rats. This species was chosen in preference to mice as they show good levels of K-cell activity. Studies were carried out in athymic (rnu/rnu) rats some of which were...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Interactions between heparin and protamine in normal human serum previously were found to activate the primary complement pathway and deplete the early-acting complement components. In the present investigation, the role of heparin-protamine complexes, immunoglobulins, C1q and C1-INH in this interaction was studied. H...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-02-01 00:00:00
abstract：:The intestinal lamina propria (LP) contains antigen-presenting cells with features of dendritic cells and macrophages, collectively referred to as mononuclear phagocytes (MNPs). Association of MNPs with the epithelium is thought to play an important role in multiple facets of intestinal immunity including imprinting M...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12801
更新日期：2017-12-01 00:00:00
abstract：:A positive association has been observed in Igh allotype "congenic' mice between skin graft rejection and a block to effective T--B co-operation in the development of both IgM and IgG responses to sheep erythrocytes. Mismatch of Igh alone is insufficient for such a block to become apparent since pairs of Igh-allotype ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:The members of the tumour necrosis factor (TNF) superfamily of cytokines play important roles in the regulation of various immune-cell functions. Likewise, induction of cell death by apoptosis is indispensable for the normal functioning of the immune system. There are two major pathways of apoptosis induction. The int...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2009.03058.x
更新日期：2009-06-01 00:00:00
abstract：:Fifteen sera of patients with visceral leishmaniasis were investigated for the occurrence of autoantibodies. They were found in high incidence and titre, and with specificity to the intermediate filament (INFIL) proteins vimentin (12 out of 15 with a titre higher than 1:10) and keratin (9 out of 15 with a titre higher...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:The class distribution of 4193 monoclonal gammopathies (MG), detected over approximately 10 years in western France (WF), was compared to that established by a compilation of twenty-seven series from the literature. Several points emerged from this study: the incidence of the light chain MG (4.8%) was slightly lower t...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:In the study described here, the effect of human tears and purified lactoferrin was investigated on the deposition of complement components on solid-phase bound immune complexes. After incubating immune complexes with fresh normal human serum, the deposition of complement components (C3, C4 and C5) was measured with a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Cancer immune surveillance is considered to be an important host protection process to inhibit carcinogenesis and to maintain cellular homeostasis. In the interaction of host and tumour cells, three essential phases have been proposed: elimination, equilibrium and escape, which are designated the 'three E's'. Several ...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2007.02587.x
更新日期：2007-05-01 00:00:00
abstract：:Neutrophil migration inhibition factor from T lymphocytes (NIF-T) is produced in response to mitogens as the result of cellular interactions between T and B lymphocytes. The requirement for B-cell alloantigen in this interaction process was indicated by (1) collaboration between peripheral blood T cells with various B...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Natural cell-mediated cytotoxicity is mediated by a family of effector cells that express cytolytic activities distinct from those generally attributed to B cells, T cells and macrophages; it includes both natural killer (NK) and natural cytotoxic (NC) activities. There is now convincing evidence to show that NC activ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:This study demonstrated that the greater infectivity of stationary-phase promastigotes of Leishmania donovani is related to increased complement fixation on the parasite surface, resulting in increased binding to host mononuclear phagocytes (MPs) via complement type 3 receptors (CR3). The in vivo infectivity of log- a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Supernatants of IL-2-activated mononuclear cells (MNC) that displayed an optimal lymphokine-activated killer (LAK) cell activity at 48-72 hr in culture were found to contain increased levels of tumour necrosis factor alpha (TNF alpha), interleukin-1 alpha (IL-1 alpha) and interferon-gamma (IFN-gamma) when compared wit...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:NKG2D is one of the best characterized activating receptors and is expressed on natural killer cells and on various T-cell subsets. This receptor recognizes several different ligands that are induced by cellular stresses. In this review, we described the mechanisms controlling the expression of NKG2D ligands, with the...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2009.03147.x
更新日期：2009-09-01 00:00:00
abstract：:Corneal allografts contain few or no Ia+ Langerhans' cells and consistently fail to elicit allospecific delayed-type hypersensitivity (DTH). The present study examined the hypothesis that the inability of corneal allografts to induce allospecific DTH was the result of active suppression. However, the results indicated...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Factors that influence the generation and maintenance of memory CD8+ T cells are not fully understood. The homeostasis of memory T cells is highly dynamic and tightly regulated by various stimuli, including cytokines and antigen-major histocompatibility complex ligands. We characterized the hepatitis C virus (HCV)-spe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2003.01769.x
更新日期：2003-12-01 00:00:00
abstract：:Mice were first primed with a type A or a type B influenza virus and then challenged intranasally at least 1 month later with another type A virus. Potent cytotoxic T cell populations were found in lung, and effector T cell function was also demonstrated in blood, spleen and mediastinal lymph nodes. Lymphocytes isolat...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-09-01 00:00:00
abstract：:Although lymphocyte traffic through the brain is normally low, this can increase dramatically in response to infection or an autoimmune reaction. We have studied the adhesion of lymphocytes to cerebral endothelium in vitro in an attempt to model the first step of the infiltration process--that is, the initial interact...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:A non-specific suppressor factor, owl monkey suppressor factor, OMSF, from leukaemic owl monkey peripheral T lymphocytes has been isolated and characterized. OMSF is a glycoprotein of a molecular weight of 66,000 which can be produced from purified owl monkey enhancing factor, OMEF, by both Tris buffer dialysis and by...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:The CD4-specific monoclonal antibody (mAb) CC26, when tested on a total of 143 cattle, failed to react with T cells from 16% of animals and gave reduced intensity staining in a further 35% of animals. The results of family studies with groups of half- and full-siblings indicated that CC26 recognizes an allele of CD4 w...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:Formation of osteoclasts and consequent joint destruction are hallmarks of rheumatoid arthritis (RA). Here we show that LIGHT, a member of the tumour necrosis factor (TNF) superfamily, induced the differentiation into tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs) of CD14(+) monocytes ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2008.02965.x
更新日期：2009-09-01 00:00:00
abstract：:The Ig subunit structure of murine B lymphocytes was studied by employing different radiolabelling techniques in combination with chemical cross-linking. The main membrane structure of IgM was a half molecule that was disulphide-linked to proteins with MW 30,000, 45,000 and 55,000, respectively. Small amounts of mu 2L...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：:Mouse models of multiple sclerosis (MS) have shown the importance of interleukin-10 (IL-10) -producing regulatory B (Breg) cells in dampening disease activity and inhibiting disease initiation and progression. In MS and other autoimmune diseases decreased frequency and functionality of Breg cells correlate with diseas...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.13024
更新日期：2019-03-01 00:00:00
abstract：:Collectins are a group of soluble proteins, each of which has collagenous and non-collagenous globular domains, and may be complement associated and/or have lectin activity. The complement protein C1q is structurally and functionally related to the collectins. Members of the collectin family are mannan-binding protein...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:B-lymphocyte maturation is considered to be independent of the thymus. However, there is circumstantial evidence suggesting that it may be impaired in nude animals that lack the thymus. Our study shows that the proportion of immature B-lymphocyte subsets (CD90(high) IgM(high) and CD90(high) IgM(low)) was significantly...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02226.x
更新日期：2005-11-01 00:00:00
abstract：:In the rat strain combination of DA into PVG, an orthotopic liver graft has the ability to abrogate an existing state of sensitization against donor (DA) antigens. Fifty-four percent of PVG rats sensitized against DA by skin grafting accepted a DA liver graft permanently, and about half of these became systemically to...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:This paper describes attempts to isolate and characterize glycosaminoglycan (GAG)-binding molecules on the surface of lymphocytes and lymphoma cell lines and relate their expression to splenic and lymph node homing capacity. Initial binding studies with radiolabelled GAG and rosetting studies with GAG-coupled erythroc...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:To evaluate the role of the high-affinity monocyte receptor for lipopolysaccharide (LPS), CD14, in the process of tolerance to LPS, the human monocytic cell line Mono-Mac-6 was cultured in the absence or presence of different amounts of LPS. The kinetics of CD14 modulation in these cells showed an initial 4-day period...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:In order to study the precise localization pattern of anti-TNP antibody-forming cells (AFCs) during the early primary immune response against TNP conjugated TD (thymus-dependent) and TI-2 (thymus-independent type-2) antigens, rats received an intravenous injection with either TNP-keyhole limpet haemocyanin (KLH) or wi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Glucosamine (GlcN) has been reported to possess several biomedical properties, and currently a great deal of attention has been focused on improving the functional properties of GlcN for different applications. Therefore, this study was conducted to introduce a carboxybutyryl functional group to GlcN and to find out t...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02683.x
更新日期：2008-03-01 00:00:00
